LOCUS       sequence-497566-        3111 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH87644
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             422..1213
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1384..1972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1873..1892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2126..2143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3026..3081
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      241 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
      301 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
      361 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
      421 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
      481 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
      541 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
      601 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
      661 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
      721 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
      781 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
      841 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
      901 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
      961 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     1021 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     1081 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     1141 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     1201 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1501 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1981 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2041 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2101 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2161 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag
     2221 gagtcgacaa aatttagaac gaacttaatt atgatctcaa atacattgat acatatctca
     2281 tctagatcta ggttatcatt atgtaagaaa gttttgacga atatggcacg acaaaatggc
     2341 tagactcgat gtaattggta tctcaactca acattatact tataccaaac attagttaga
     2401 caaaatttaa acaactattt tttatgtatg caagagtcag catatgtata attgattcag
     2461 aatcgttttg acgagttcgg atgtagtagt agccattatt taatgtacat actaatcgtg
     2521 aatagtgaat atgatgaaac attgtatctt attgtataaa tatccataaa cacatcatga
     2581 aagacacttt ctttcacggt ctgaattaat tatgatacaa ttctaataga aaacgaatta
     2641 aattacgttg aattgtatga aatctaattg aacaagccaa ccacgacgac gactaacgtt
     2701 gcctggattg actcggttta agttaaccac taaaaaaacg gagctgtcat gtaacacgcg
     2761 gatcgagcag gtcacagtca tgaagccatc aaagcaaaag aactaatcca agggctgaga
     2821 tgattaatta gtttaaaaat tagttaacac gagggaaaag gctgtctgac agccaggtca
     2881 cgttatcttt acctgtggtc gaaatgattc gtgtctgtcg attttaatta tttttttgaa
     2941 aggccgaaaa taaagttgta agagataaac ccgcctatat aaattcatat attttcctct
     3001 ccgctttgaa tactgtattt ttacaacaat taccaacaac aacaaacaac aaacaacatt
     3061 acaattacta tttacaatta caatgtgaga ccacgaagtg gctcttcagt g
//
