LOCUS       sequence-497567-        3105 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71311
FEATURES             Location/Qualifiers
     source          1..3105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(311..1102)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1324..1428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1496..1514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2486..2503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2657..3105,1..140))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2737..2756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
       61 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttatttgccg actaccttgg tgatctcgcc ttttacgtaa tggacaaatt
      361 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
      421 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
      481 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
      541 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
      601 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
      661 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
      721 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
      781 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
      841 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
      901 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
      961 tatcgctgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     1021 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     1081 cggcgatcac cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg
     1141 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     1201 tggatgcccg aggcatagac tgtaccccaa aaaaaaacag tcataacaag ccatgaaaac
     1261 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     1321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1441 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1501 gcgtatcacg aggccctttc gtccactgaa gagccacttc gtggtctcac attgctgaaa
     1561 accaagtgct tatcttgaac tcaagagggt tattgctttc tagtctctcc ctctatatat
     1621 aatgataagg tttcacttaa ctttcccatc taacggacac aaattaacca acccatttgt
     1681 aaccattgga tcatgccctt gttcacattt caaaagtaga attttatcga atctattaag
     1741 tagaattata actccatctt aatgacatta atttgggcgt ggagggtggg tgagctataa
     1801 taataattag atatattaat taagccacaa gaaagggtgc cacgtgtcag aatgagaata
     1861 gtcttatctc atcctaaaag gggtaaggaa tgagtgaaaa tggttggtta atttcatttt
     1921 gggtcagtgt tttatttgtt ttgttgtcat tctgaattta tattggctgc tactttgtag
     1981 ccgttttgct tgcatttgga gccattcctt tgggcagaca cattcagatt ttcttttgtt
     2041 tccatgcaaa tcattttatt ttttttgtaa tttcaagttc aaaatcaaat attgttcctt
     2101 ggttgcaact tggaaatgtt cttatccttt gtatgtgtgg aacataacat ctcctagttt
     2161 ctatatatat gaaaaggttt tcttttattt ccaataaaaa acaattaaaa aatcaggtag
     2221 tttattattt cttatatttt agaccaaata aattagaaca agtaattttc cgtagtttcc
     2281 tgtataatgg attttttttc tcgaattttt ctcttagcga ccccaagaag caagattgat
     2341 gttgtctaat tagatcacga attacaaatg tacgaaaaag ttttaccgct atgtcacgta
     2401 gcaatctcct gagaccacag agtgattaat gaatcggcca acgcgcgggg agaggcggtt
     2461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2521 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2581 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2641 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2701 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2761 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2821 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2881 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2941 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3001 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3061 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtat
//
