LOCUS       sequence-497569-        2388 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM1467
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(123..914)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1136..1240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1308..1326
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(1365..1379)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1389..1406)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1581..1598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1752..2340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1832..1851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
       61 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      121 agttatttgc cgactacctt ggtgatctcg ccttttacgt aatggacaaa ttcttccaac
      181 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
      241 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
      301 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
      361 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
      421 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
      481 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
      541 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
      601 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
      661 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
      721 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg
      781 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
      841 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
      901 accgcttccc tcatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
      961 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1021 cgaggcatag actgtacccc aaaaaaaaac agtcataaca agccatgaaa accgccactg
     1081 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatactct
     1141 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1201 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1261 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1321 cgaggccctt tcgtccactg aagagccact tcgtggtctc acattttgtc gtcgtcgtcg
     1381 ccgctgctgt ggtggtggtg gtggtggctg ctgcccatgt atcgataatt gtaaatgtaa
     1441 ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatga gctcttatac
     1501 agtatgagac cacagagtga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1561 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1621 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1681 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1741 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1801 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1861 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1921 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1981 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2041 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2101 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2161 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2221 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2281 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2341 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaa
//
