LOCUS       sequence-49757-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6872 pHAGE-N-V5-MAML1-FL
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(284..308)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(324..365)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(464..488)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    498..516
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    519..537
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(539..742)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(568..588)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcggcgtcca ggcgcggggc cggggcggcg ggcgccgggg ccgtggcggc gggcggctgc
       61 ctgtgcttcc cggcgcgctt ggccttggcc tggatgcagc gctggtgcag ggcgaaggtg
      121 tgttggcgct ccagctccag gcgctcgggc gacacggcct cgtagcgggc ctcgcaggtg
      181 ctgtggtggc gccggcacag ctcgatgcgc cggcgaaggc gctccatgac cgcgctgtgc
      241 cgcggcagcg cgaactccgc catggggcag gtgggcagca ccatgccaac ttttttgtac
      301 aaacttgttt gataacctcg agccgtagaa tcgagaccga ggagagggtt agggataggc
      361 ttacccgcca tggtggctag cgtttaaacg ctagagtccg gaggctggat cggtcccggt
      421 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
      481 aacgagctcg tcgacgatct ctatcactga tagggagatc tctatcactg atagggagag
      541 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      601 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      661 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      721 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      781 aag
//
