LOCUS       sequence-497572-        2469 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH72400
FEATURES             Location/Qualifiers
     source          1..2469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(124..228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     296..314
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(354..554)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     primer_bind     complement(650..667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(821..1409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(901..920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1580..2371)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggatgcccg aggcatagac tgtaccccaa aaaaaaacag tcataacaag ccatgaaaac
       61 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
      121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      241 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      301 gcgtatcacg aggccctttc gtccactgaa gagccacttc gtggtctcaa gcgatcttga
      361 aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat agtccaagca
      421 aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac tgattatatc
      481 agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatgtgtaat acataaattg
      541 atgatatagc tagctgtcga cgggggatca agctgagacc acagagtgat taatgaatcg
      601 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      661 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      721 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1261 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1501 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1561 agtaaacttg gtctgacagt tatttgccga ctaccttggt gatctcgcct tttacgtaat
     1621 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     1681 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     1741 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     1801 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1861 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1921 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1981 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     2041 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     2101 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     2161 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     2221 gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     2281 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     2341 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc
     2401 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     2461 cttgctgct
//
