LOCUS       sequence-497573-        3052 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45173
FEATURES             Location/Qualifiers
     source          1..3052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(455..1246)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1468..1572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1640..1658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2577..2594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2748..3052,1..284))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2828..2847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
       61 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      421 tctaaagtat atatgagtaa acttggtctg acagttattt gccgactacc ttggtgatct
      481 cgccttttac gtaatggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
      541 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
      601 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
      661 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
      721 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
      781 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
      841 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
      901 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
      961 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     1021 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1081 cggtcaccgt aaccagcaaa tcaatatcgc tgtgtggctt caggccgcca tccactgcgg
     1141 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     1201 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg
     1261 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1321 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaaa
     1381 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1441 ggttctggac cagttgcgtg agcgcatact cttccttttt caatattatt gaagcattta
     1501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     1621 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtccac tgaagagcca
     1681 cttcgtggtc tcaagtataa tgatcaaagg gtccgggatt catgataact cgttcactac
     1741 cacatgatta aggaaaggtg ttgacaccct tatcttatct ttcttttgtg aggaaatgag
     1801 tgattgtgta tggtttacta gtctccactt gatcggttgc tttggctcct tccttttgga
     1861 agaaaagaaa tcagatgcat gaccgtaaat ataatgtttt ggacctttta tttatcttgt
     1921 atgtattttt aaaatttggt ttacattact tcggttaata aatactataa caggaatatc
     1981 tataaacaga gtcatgactc ataatgcatt tcacaatgtc aaagctgaac gttgagataa
     2041 atttatcaat agcactaaat attaatccgc ggtataccgc gggtctatat tttcattaaa
     2101 taaaaaataa ttgaatattt cttaggatga cttaataaat ttctgctatt gtttgattta
     2161 aatgtataat tcaccgcgag acaaaatttt ataactcaaa ttttaaagtt ttaagttgta
     2221 tttgtttatt ttgttaaatg tttaatattg tataattgta ttttgattgt tgtttctcgg
     2281 atttcacccg tagtacacca tcccatatta atatcgaatc aaacccgtca attctaggat
     2341 ttcacctgtg gtagtattta attgtataat aataacttga ttgtcattct aggatttcac
     2401 tcctagttct atcgcaaatt attatcaact caaaccagtc aattctaaaa tttcacccgt
     2461 tgtacaccat cccatattaa tatcgaatca agcccgctcc tgagaccaca gagtgattaa
     2521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tc
//
