LOCUS       sequence-497574-        3677 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH51266
FEATURES             Location/Qualifiers
     source          1..3677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..26
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1096..1441
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1384..1404
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1570..1588)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1656..1760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1982..2773
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2944..3532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
       61 tccccgcgcg ttggccgatt cattaatcac tctgtggtct caggaggaat tccaatccca
      121 caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac
      181 cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg
      241 ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact
      301 attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg
      361 aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca
      421 agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat
      481 ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt
      541 tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat
      601 gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
      661 tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac
      721 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca
      781 gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      841 cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa
      901 tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa
      961 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     1021 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     1081 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     1141 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     1201 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
     1261 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
     1321 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     1381 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     1441 acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     1501 cattacaatt acatttacaa ttatcgatac aatgtgagac cacgaagtgg ctcttcagtg
     1561 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1621 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1741 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1801 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
     1861 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1921 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     1981 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2041 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2101 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2161 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2221 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2281 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2341 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2401 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2461 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2521 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2581 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2641 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2701 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     2761 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacga
//
