LOCUS       sequence-497575-        3003 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45195
FEATURES             Location/Qualifiers
     source          1..3003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     357..374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1244..1262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1330..1434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1656..2447
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2618..3003,1..203)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
       61 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      421 ggccgattca ttaatcactc tgtggtctca ggaggcttaa gtgggccgta agtgcaattg
      481 cgttgtgctc caaaaggaac ggtgccgtgg gttcctcttg ttcatcagaa atatattaat
      541 tagccgtaaa acctgaaaat tcacaagcat ttggattgtt ttctaattta atatccatta
      601 tgtgactaaa agttctagtg atcgtacata ctacatagaa aataataaca caaaatacta
      661 gtttacattt cccaattaaa aaccattttg aatgaactct gtctgattta attatacttt
      721 taaaatgtgg gatgaattca aagattatac ttatattctt attatttaag attatcaagt
      781 ggaaaaataa aaatatgaat gtgttaatat aaggtaatag aaatttaatc atttttttaa
      841 tctatatgta aaaagtattt aaccgatatc tacaatttga cgcctcccaa ttgaaaggag
      901 ccaaaagcaa ccgatcaagt gcagaccagt agccatacac attcactcct acccttacat
      961 gagaaagata agattatgga gttttctgcc acgtgatctt atcctagtgg tccaaatcga
     1021 taagggtgtc aacacctttc cttaatcctg tggcaattaa cgacgttatc atgaattatg
     1081 gcccctttga tcattagggc tagttgcctc tagcggttcc cactatataa agatgacaaa
     1141 accaacagac aaacaagtaa gtaagagaaa aaccaaaaga agaagagaaa caacaagaag
     1201 aagtaatgtg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
     1261 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1321 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1381 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc
     1441 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1501 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc
     1561 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1621 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     1681 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1741 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg
     1801 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1861 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     1921 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1981 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2041 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2101 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2161 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2221 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2281 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2341 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2401 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag
     2461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2761 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2881 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3001 agc
//
