LOCUS       sequence-497576-        2367 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM1479
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(164..752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(244..263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(923..1714)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1936..2040)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2108..2126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(2171..2188)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(join(2360..2367,1..10))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
       61 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
      901 atgagtaaac ttggtctgac agttatttgc cgactacctt ggtgatctcg ccttttacgt
      961 aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     1021 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     1081 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     1141 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     1201 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     1261 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     1321 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     1381 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     1441 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     1501 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     1561 ccagcaaatc aatatcgctg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     1621 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     1681 gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt ttagggcgac
     1741 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     1801 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaaaac agtcataaca
     1861 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     1921 gttgcgtgag cgcatactct tcctttttca atattattga agcatttatc agggttattg
     1981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2041 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2101 ctataaaaat aggcgtatca cgaggccctt tcgtccactg aagagccact tcgtggtctc
     2161 acattccgct gtggtggtgg tggtggtggc tgcccatgta tcgataattg taaatgtaat
     2221 tgtaatgttg tttgttgttt gttgttgttg gtaattgttg taaaaatgag ctcttataca
     2281 gtatgagacc acagagtgat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2341 ttgggcgctc ttccgcttcc tcgctca
//
