LOCUS       sequence-49759-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6873 pHAGE-N-V5-MAML1 (1-990)
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(285..309)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(325..366)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(465..489)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    499..517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    520..538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(540..744)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(569..589)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agcggcgtcc aggcgcgggg ccggggcggc gggcgccggg gccgtggcgg cgggcggctg
       61 cctgtgcttc ccggcgcgct tggccttggc ctggatgcag cgctggtgca gggcgaaggt
      121 gtgttggcgc tccagctcca ggcgctcggg cgacacggcc tcgtagcggg cctcgcaggt
      181 gctgtggtgg cgccggcaca gctcgatgcg ccggcgaagg cgctccatga ccgcgctgtg
      241 ccgcggcagc gcgaactccg ccatggggca ggtgggcagc accatgccaa cttttttgta
      301 caaacttgtt tgataacctc gagccgtaga atcgagaccg aggagagggt tagggatagg
      361 cttacccgcc atggtggcta gcgtttaaac gctagagtcc ggaggctgga tcggtcccgg
      421 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
      481 aaacgagctc gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga
      541 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      601 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      661 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      721 atgtactgcc aaaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
      781 ccaagtagaa
//
