LOCUS       sequence-49763-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6874 pHAGE-N-V5-MAML1-ADDAA
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(281..305)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(321..362)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(461..485)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    495..513
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    516..534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(536..741)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(565..585)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcgtccaggc gcggggccgg ggcggcgggc gccggggccg tggcggcggg cggctgcctg
       61 tgcttcccgg cgcgcttggc cttggcctgg atgcagcgct ggtgcagggc gaaggtgtgt
      121 tggcgctcca gctccaggcg ctcgggcgac acggcctcgt agcgggcctc gcaggtgctg
      181 tggtggcgcc ggcacagctc gatgcgccgg cgaaggcgct ccatgaccgc gctgtgccgc
      241 ggcagcgcga actccgccat ggggcaggtg ggcagcacca tgccaacttt tttgtacaaa
      301 cttgtttgat aacctcgagc cgtagaatcg agaccgagga gagggttagg gataggctta
      361 cccgccatgg tggctagcgt ttaaacgcta gagtccggag gctggatcgg tcccggtgtc
      421 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
      481 gagctcgtcg acgatctcta tcactgatag ggagatctct atcactgata gggagagctc
      541 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      601 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      661 caatgggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      721 tactgccaaa aaccgcatca ccatggtaat agcgatgact aa
//
