LOCUS       sequence-497715-       10621 bp    DNA     circular SYN 16-JAN-2026
DEFINITION  For all-in-one Tet-Off inducible expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10621)
  TITLE     Prohibitin inhibits DELE1-mediated integrated stress response by 
            blocking Oma1 activation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-TetOne-Off-PHB2-mStayGold-blast
FEATURES             Location/Qualifiers
     source          1..10621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    169..303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        351..455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(456..524,525..1316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(674..693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1487..2075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1976..1995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2229..2246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2363..2384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2399..2404,2405..2422,2423..2429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2437..2453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2442..2464
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2461..2477
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2461..2477
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2605..3238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3445..4033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3551..3562
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3960..3980
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4047..4445)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4454..4783)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4468..4603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5559..6069)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5622..5640)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        6086..6453
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6094..6112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6130..6148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6166..6184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6202..6220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6238..6256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6274..6292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6310..6328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      6460..6469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7441..8118
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTL
                     QYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHL"
     polyA_signal    8302..8436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8480..8595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8858..8902)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9087..9320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9817..9942
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(9989..10621,1)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aactactaag tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc
       61 agaggaatca caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta
      121 tggggtggga agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg
      181 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      241 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      301 gttgatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      361 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      421 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      481 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      541 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      601 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      661 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      721 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      781 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      841 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      901 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      961 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1621 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2161 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2221 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2281 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2341 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2401 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2461 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtaaacgct
     2521 agcaatgtca aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     2581 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     2641 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     2701 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     2761 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     2821 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     2881 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     2941 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     3001 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     3061 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     3121 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     3181 tggtagatcc acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt
     3241 cccctctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     3301 aaaggtacct gaggtgtgac tggaaaaccc acctcctcct cctcttgtgc ttctagccag
     3361 gcacaatcag cattggtagc tgctgtattg ctacttgtga ttgctccatg tttttctagg
     3421 tctcgactgc agaattaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct
     3481 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
     3541 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     3601 tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat
     3661 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     3721 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     3781 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     3841 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     3901 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     3961 atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat
     4021 ccagaggttg attgttccag acgcgtttag ccctcccaca cataaccaga gggcagcaat
     4081 tcacgaatcc caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca
     4141 ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc
     4201 tcatttccga tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc
     4261 agcaccacga gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag
     4321 atgcggccgt cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg
     4381 ctgttgattg tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg
     4441 gccatggtcg cgttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     4501 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     4561 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     4621 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     4681 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     4741 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattcgcg cgtttacccg
     4801 gggagcatat caaggtccag atcgtccagt gcatcggcgg ggagcatgtc caggtcaaaa
     4861 tcatccaggg cgtcagcggg cagcatatcc agatcgaagt cgtccagagc gtcggcaggt
     4921 ccgccagatt cgcacttcag ctgcttctcg aggccgcaga tgatcagttc caggccaaac
     4981 aggaaggcag gttcggcgcc ttggtgatcg aacagctcga tggcctgtct cagcagtgga
     5041 ggcatagaat cggtggtagg tgtctctctt tcctcttttg ctacttgatg ctcctgatcc
     5101 tccaatacgc agcccagtgt aaagtggccc acggcggaca gagcgtacag tgcgttctcc
     5161 agggagaagc cttgctgaca caggaacgcg agctgatttt ccagggtttc gtactgtttc
     5221 tctgttgggc gggtgccgag atgcacttta gccccgtcgc gatgtgagag gagagcacag
     5281 cggaatgact tggcgttgtt ccgcagaaag tcttgccatg actcgccttc cagggggcag
     5341 aagtgggtat gatgcctgtc cagcatctcg attgccaggg catcgagcag ggcccgcttg
     5401 ttcttcacgt gccagtacag ggtaggctgc tcaactccca gcttttgagc gagtttcctt
     5461 gtcgtcaggc cttcgatacc gacttcattg agtaattcca gagcagagtt tatgactttg
     5521 ctcttgtcca gtctagacat ggtaattcga tgatccccct ggggagagag gtcggtgatt
     5581 cggtcaacga gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac
     5641 cgcgcgggca ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct
     5701 tcccggccgc tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc
     5761 ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt
     5821 gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg
     5881 ggcggagcag gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg
     5941 ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa
     6001 ccacgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac
     6061 cccaaccccg tggaattatc acctcgagtt tactccctat cagtgataga gaacgtatga
     6121 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     6181 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     6241 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     6301 ccagtttact ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg
     6361 cctataaaag agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata
     6421 ccaactttcc gtaccacttc ctaccctcgt aaagaattcg ccaccatggc tcagaatctg
     6481 aaagacctgg ccggcagact gcctgccgga cctagaggaa tgggaacagc cctgaaactg
     6541 ctgcttggag ctggcgctgt ggcttatggc gtcagagaaa gcgtgttcac cgtggaaggc
     6601 ggtcatagag ccatcttttt taatcgtatt ggtggcgtgc agcaggacac gatcctggcc
     6661 gaaggccttc acttcaggat cccctggttc cagtacccca tcatctatga cattcgggcc
     6721 agacctcgaa aaatctcctc ccccacaggc tccaaagacc tgcagatggt gaacatctcc
     6781 ctgcgtgtgc tgtcccgacc caatgcccag gagctcccca gcatgtacca gcgtctaggg
     6841 ctggactatg aggagcgagt gctgccgtcc attgttaatg aggtgctcaa gagtgtggtg
     6901 gccaagttca atgcctcgca gctgatcacc cagcgggctc aggtgtccct gttgatccga
     6961 agagagctga cagagcgcgc caaggacttc agcctcatcc tggatgatgt agctatcaca
     7021 gagctgagct tcagccgaga gtacacagct gctgtagaag ccaagcaagt ggcccagcag
     7081 gaagcccagc gggcccagtt tttggtggag aaagcgaagc aggaacagcg acagaagatt
     7141 gtgcaggctg agggggaggc ggaggctgcc aagatgcttg gagaagcact gagcaagaat
     7201 cctggctata tcaagctccg aaagatccgg gccgcccaga acatctctaa aacgatcgcc
     7261 acatcacaga accgaatcta tctcacagct gacaaccttg tgctgaatct acaggatgaa
     7321 agttttactc ggggaagtga cagcctcatt aagggtaaga agctcgaggg tggaggcggt
     7381 tcaggcggag gtggctctgg cggtggcgga tcgatcgaat tcctgcagcc cgggggatcc
     7441 atggtgtcta caggcgagga gctgtttacc ggcgtggtgc ccttcaagtt ccagctgaag
     7501 ggcaccatca acggcaagag cttcaccgtg gaaggcgagg gcgagggcaa tagccacgag
     7561 ggcagccaca aaggcaagta cgtgtgcacc agcggcaaac tgccaatgtc ttgggccgcc
     7621 ctgggaacta gcttcggcta tggcatgaag tactacacca agtaccccag cggcctgaag
     7681 aactggttcc acgaggtgat gcccgagggc ttcacctacg acagacacat ccagtacaag
     7741 ggcgacggca gcatccacgc caagcaccag cacttcatga agaacggcac ctaccacaac
     7801 atcgtggagt tcaccggcca ggacttcaag gagaacagcc ccgtgctgac cggcgacatg
     7861 gacgtgagcc tgcccaacga ggtgcagcac atccccatag atgacggcgt ggagtgcaca
     7921 gtgaccctgc agtaccctct gctgagcgac gagagcaagt gcgtggaggc ctaccagaac
     7981 accatcatca agcccctgca caatcagcca gcccccgatg tgccattcca ctggatcaga
     8041 aagcagtaca cccagagcaa ggacgacacc gaggagagag accacatcat ccagagcgag
     8101 accctggagg cccacctgta aggatccaga ccacctcccc tgcgagctaa gctggacagc
     8161 caatgacggg taagagagtg acatttttca ctaacctaag acaggagggc cgtcagagct
     8221 actgcctaat ccaaagacgg gtaaaagtga taaaaatgta tcactccaac ctaagacagg
     8281 cgcagcttcc gagggatttg agatccagac atgataagat acattgatga gtttggacaa
     8341 accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc
     8401 ttatttgtaa ccattataag ctgcaataaa caagttaagt cgataaactg gatctctgct
     8461 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     8521 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     8581 cccttttctt ttaaaggcga taccgtcgag atccgttcac taatcgaatg gatctgtctc
     8641 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
     8701 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     8761 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     8821 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     8881 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     8941 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     9001 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     9061 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     9121 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     9181 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     9241 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     9301 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     9361 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     9421 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccggccgc
     9481 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     9541 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     9601 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     9661 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     9721 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     9781 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     9841 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     9901 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     9961 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
    10021 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
    10081 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
    10141 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
    10201 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg gaaagtcccc
    10261 agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact ccggatgcag
    10321 ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta acacttctct
    10381 ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt tctctccttt
    10441 attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc atccaaaggt
    10501 cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga agtagccttg
    10561 tgtgtggtag atccacagat caaggatatc ttgtcttctt tgggagtgaa ttagcccttc
    10621 c
//
