LOCUS       sequence-497725-       13342 bp    DNA     circular SYN 16-JAN-2026
DEFINITION  Constitutively expresses myc tagged membrane bound IL15, miRFP720 
            and a blasticidin resistance gene in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Deol S, Donahue PS, Mitrut RE, Hammitt-Kess IJ, Ahn J, Zhang B, 
            Leonard JN
  TITLE     Comparative Evaluation of Synthetic Cytokines for Enhancing 
            Production and Performance of NK92 Cell-Based Therapies.
  JOURNAL   GEN Biotechnol. 2023 Jun 1;2(3):228-246. doi: 
            10.1089/genbio.2023.0024. Epub 2023 Jun 19.
  PUBMED    37363412
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2023.0024"; journalName: "GEN Biotechnol"; date: 
            "2023-06-1- 1"; volume: "2"; issue: "3"; pages: "228-246"
COMMENT     Sequence Label: pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A...
FEATURES             Location/Qualifiers
     source          1..13342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2865..4047
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3096..4038
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3995..4015
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     4049..4073
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4148..4157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5348..5377
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(5396..5970,5971..5973,5974..5982)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5563..5580)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5790..5809
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6952..7008
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7021..7419
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7437..8025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7490..7510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7908..7919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8112..8345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8446..8463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8452..8676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8722..9150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8809..8828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9019..9040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9159..9179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9164..9493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9344..9479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9406..9425
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9542..10567
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVHADSGNRRPSTRPRAK
                     E"
     polyA_signal    10697..10818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10867..10883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10867..10883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10880..10902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10891..10907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10915..10921,10922..10939,10940..10945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10960..10981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11098..11115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11269..11857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11349..11368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12028..12819,12820..12888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12651..12670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12889..12993)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13041..13175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg
     2761 accgtcgtta aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac
     2821 ccgcccgctc tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc
     2881 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2941 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     3001 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     3061 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     3121 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg
     3181 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     3241 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg
     3301 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3361 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3421 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3481 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3541 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3601 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3661 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     3721 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     3781 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     3841 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3901 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3961 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     4021 ttttttcttc catttcaggt gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga
     4081 gacgccatcc acgctgtttt gacctccata gaagagaacg ggaccgatcc aaattctcga
     4141 ggctagcgcc accatggact ggacctggat tctgtttctg gtggccgctg ccacaagagt
     4201 gcatagcaac tgggtgaacg tgatcagcga cctgaagaag atcgaggacc tgatccagtc
     4261 tatgcacatc gacgccacac tgtataccga gtccgatgtt cacccctcct gcaaagtgac
     4321 cgccatgaag tgctttctgc tggaactgca agtgatcagc ctggaaagcg gcgacgccag
     4381 catccacgac accgtggaaa acctgatcat cctggccaac aacagcctga gcagcaacgg
     4441 caatgtgacc gagagcggct gcaaagagtg cgaggaactg gaagagaaga atatcaaaga
     4501 gttcctccag agcttcgtcc acatcgtgca gatgttcatt aacaccagca gcggcggagg
     4561 atctggcgga ggtggaagcg gaggcggagg aagcggtggc ggaggtagtg gcggaggatc
     4621 acttcagatc acctgtcctc cacctatgag cgtggaacac gccgacatct gggtcaagag
     4681 ctacagcctg tactccagag agcggtacat ctgcaacagc ggcttcaaga gaaaggccgg
     4741 aaccagcagc ctgaccgagt gcgtgctgaa caaggctacc aatgtggccc actggaccac
     4801 acctagcctg aagtgcatca gagatcccgc tctggtgcat cagcgtcctg ctccacctag
     4861 cacagtgaca acagcaggcg tgacacccca gcctgagtct ctgagccctt ctggaaaaga
     4921 gcctgccgcc agctctccca gctctaacaa tactgccgcc accacagccg ccatcgtgcc
     4981 tggatctcaa ctgatgccta gcaagagccc tagcaccggc accacagaga tcagctctca
     5041 cgagtctagc cacggcaccc ctagccagac cacagccaag aattgggagc tgacagccag
     5101 cgcctctcat caaccacctg gcgtgtaccc acagggccac agcgatacaa cagtggccat
     5161 cagcacaagc accgtgctgc tgtgtggact gtctgccgtt tctctgctgg cctgctacct
     5221 gaagtccaga cagacacctc cactggccag cgtggaaatg gaagctatgg aagccctgcc
     5281 tgtgacctgg ggcacctcta gtagagatga ggacctcgaa aactgctccc accacctggg
     5341 ctcaggcgaa cagaagctca tcagcgaaga ggacctctga gcggccgcgg atccgcccct
     5401 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5461 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5521 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5581 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5641 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5701 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5761 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5821 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     5881 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5941 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgggaac tagtatggcc
     6001 gagggctctg ttgctagaca acccgatctg ctgacctgcg acgacgagcc cattcatatc
     6061 cctggcgcta tccagcctca cggactgctg cttgctctgg ccgccgatat gacaatcgtg
     6121 gccggcagcg ataacctgcc tgagctgaca ggactggcca tcggagccct gattggaaga
     6181 tcagccgccg acgtgttcga cagcgagaca cacaacagac tgacaatcgc cctggccgaa
     6241 cctggtgctg ctgtgggagc acctatcacc gtgggcttca ccatgagaaa ggacgccggc
     6301 tttatcggca gctggcacag acacgaccag ctgatctttc tggaactgga acctcctcag
     6361 agagatgtgg ccgagcctca ggccttcttc agaaggacca acagcgccat cagacggctc
     6421 caggccgctg agacactgga atctgcttgt gccgccgctg ctcaagaagt gcggaagatc
     6481 accggcttcg accgcgtgat gatctacaga ttcgccagcg acttcagcgg cgaagtgatc
     6541 gccgaggata gatgcgccga ggtggaatct aagctgggcc tgcattaccc cgccagcttc
     6601 attcctgctc aggccagacg gctgtatacc atcaatcccg tgcggatcat ccccgacatc
     6661 aactacagac ccgtgcctgt gactcccgat ctgaaccctg ttaccggcag acccatcgac
     6721 ctgagcttcg ccatcctgag atccgtgtct cccgtgcatc ttgagttcat gcggaacatc
     6781 ggaatgcacg gcaccatgag catctctatc ctgcggggcg aaagactgtg gggcctgatc
     6841 gtgtgtcacc acagaacccc ttactacgtg gacctggatg gcagacaggc ctgcaaaaga
     6901 gtggccgaga gactggccac acagatcggc gtgatggaag aaggtagtgg agcaactaac
     6961 ttttccctgc tcaaacaagc cggggatgta gaggagaacc ccggccccgc cccccttaag
     7021 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     7081 aacagcatcc ccatctctga ggactacagc gtcgccagcg cagctctctc tagcgacggc
     7141 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     7201 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     7261 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     7321 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     7381 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaat gtacaagtag gtcgacaatc
     7441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7801 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc
     8041 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8101 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     8161 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8221 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8281 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8341 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8401 atcagagagt gagaggcctt gacattgttt aaacccgctg atcagcctcg actgtgcctt
     8461 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8521 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8581 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8641 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8701 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8761 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8821 tcttcccttc ctttctcgtc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8881 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8941 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9001 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9061 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9121 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9181 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9241 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9301 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9361 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9421 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9481 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     9541 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     9601 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     9661 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     9721 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     9781 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     9841 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     9901 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     9961 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    10021 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    10081 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    10141 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    10201 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    10261 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
    10321 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    10381 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    10441 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    10501 ctgtgtagaa gtacacgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    10561 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
    10621 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    10681 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10801 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
    10861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    11041 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11701 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    12001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    12061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    12121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    12181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    12241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    12301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    12361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    12421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    13021 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    13081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13141 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    13201 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    13261 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    13321 tatgtactac aaacttagta gt
//
