LOCUS       sequence-497739-        7676 bp    DNA     circular SYN 16-JAN-2026
DEFINITION  Cre-dependent AAV expressing light-activatable FGFR1 (optoFGFR1-HA) 
            for temporally precise control of neuronal FGFR1 signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Shin J, Oh H, Park JH, Bae H, Yang CM, Lee M, Kim S, Heo WD
  TITLE     Dysregulation of FGFR1 signaling in the hippocampus facilitates 
            depressive disorder.
  JOURNAL   Exp Mol Med. 2025 Aug;57(8):1818-1836. doi: 
            10.1038/s12276-025-01519-9. Epub 2025 Aug 15.
  PUBMED    40813470
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s12276-025-01519-9"; journalName: "Exp Mol Med"; date: 
            "2025-08"; volume: "57"; issue: "8"; pages: "1818-1836"
COMMENT     Sequence Label: AAV-hSyn-DIO-optoFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..7676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        146..593
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(612..645)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(696..729)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(751..777)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3651..3660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3662..3695
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3746..3779
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3804..4392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3857..3877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4275..4286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4424..4900
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4623..4642)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4940..5080
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4940..5069
                     /label=AAV2 ITR
     rep_origin      5155..5610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5242..5261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5452..5473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5627..5646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5746..5768
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5806..5824)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5892..5996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5997..6065,6066..6857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6215..6234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7028..7616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7517..7536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtagtgc aagtgggttt taggaccagg atgaggcggg
      181 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      241 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      301 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      361 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      421 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      481 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      541 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtctagag
      601 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg
      661 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata
      721 cgaagttatg ggcggccgcg gcgcgcctta agcataatct ggaacatcat atggatatac
      781 cggtgggctg ccgctgccgg cagcaccgat cataatctgc gcctcccgag tgcgagagat
      841 cgctttagcc agcagctcgc gtgctgtatc tatatcaact ataggcttag cgtaattggt
      901 gcccagctcc accccgcttg ccttgagcac tgtcagtggt gcgtcccaag gatggtgaat
      961 ccactccgta ggaagtcggg ccagctcggg gagccactgt cgaatatact ctccttcggg
     1021 atcatactta gctccctgca aggccggatt gtccaacctg tccaactcat ggccgtcagg
     1081 aattgacccg ctaatatatt gccatcccag aatgtcacac tccaagtcgg cgtcgagaag
     1141 ggtatcccaa aagtacttca ttccccattt ccaaggcagg agcagaaact tgacagcaaa
     1201 tgaggacacg attacccgga tgcggttgtg catccagcct gtagcccaga gctctctcat
     1261 gccagcatcc acgagtgggt accctgtcct tccctgtctc catgccttga atttgtcgac
     1321 atcggcgtcc caggggaaga acctgaggtg ggacaacaga ctctgctcgt gtgtgaatgg
     1381 aaagttgaaa catatatacc gggagtattc ccgaagtccg atccctcgca ggaacagatc
     1441 ggcgctttcg gcgccttcgc tgtttttatc gcgagcccag ataatctgtt tcatccgagc
     1501 gcactgaaat acatggcgga cagaaatctc gccaaaatgg agatatggag agagcaagct
     1561 agttgagtta cctaccactt tcttggagtt cttcgcgtag tcaatcagtt gtttttcgat
     1621 gaactcgtta agcagcttgt cggcattaga ccagccgggg ctccaggctc tagtgagaag
     1681 tgcattgctt ggcttttctg cttcattttc cagaccgagt tcctcgatgg agcaggccca
     1741 gatggcctct gcagcagcag taatcggcat cagtctccag ggagggggca acataacgga
     1801 ctctattgac atatccaaac atttcttcca gtaactgttg aaggaggtga acggtttccc
     1861 tttctcgcaa tagatctccc atggctcgta cagaaggtcc ccgttgtagc tctgcacact
     1921 gatcccccgt tccaccagct tctccttcac agtgtggtcg cgcaccagac taactggatc
     1981 gtacaggtgg ttaaacacga ctttcgttgc gccggtcacc ctgatgcagt cgaggatggc
     2041 agagatggta ttgtgtgtct tgatcagggt aagatcactg cccagtgctt tcagtgactg
     2101 gctaagatgg gccagacttt gcttcatcca ccacctagag gcccttccgg gataaaactg
     2161 gccttcttct tcaggacacc aaatgaaaac ggggaagacg gaaccctcgt gggccgcggc
     2221 ggcgagcgcg ggattatctt ctattctcaa atctctccga aaccagacga tggtcttttt
     2281 gtccatcttc atgggatccg cgcggcgttt gagtccgcca ttggcaagct gggctgggtg
     2341 tcggggcagg cagggctcct cgggcagcgg ctcatgagag aagacggaat cctcccctga
     2401 ggagcacgta gagctccggg tgtcgggaaa gctgggggag tactggtcca ggggcatgga
     2461 caggtccagg tactcctggt tggaggtcaa ggccacgatg cggtccaggt cttccaccag
     2521 ctgcttgaag gtgggtctct gtgagggcac tgcatgccag cagtcccgca tcatcatgta
     2581 cagctcgttg gtgcagttac tgggcttgtc catgcggtga ccctccttca gcagcttgaa
     2641 aagttcctcc acaggcacac cggggtatgg ggagccgccc agagtgaaga tctcccacag
     2701 gagcaccccg aaagaccaca catcactctg gtgggtgtag atccggtcaa ataatgcctc
     2761 gggtgccatc cacttcacag gcagtcggcc gttggttgtc tttttatagt agtcgatgtg
     2821 gtgaatgtcc cgtgcgaggc caaagtctgc tatcttcatc acattgtcct ctgtcaccag
     2881 gacattcctg gctgccaggt ctcggtgtat gcacttcttg gaggccagat actccatgcc
     2941 tcgggccacc tggtaggcgc aggacaccag gtccttggag gagagctgct cctctgggtt
     3001 gtggctgggg ttgtagcagt attccagccc tgggggcctc cgggcctgca ggtactcccg
     3061 caggttgccc ttggaggcat actccacgat gacatacaag ggaccatcct gcgtgcaggc
     3121 ccccagcagg ttgatgatat tcttatgctt cccgatcatc ttcatcatct ccatttctga
     3181 gatcaggtct gacaagtctt tctctgttgc gtccgacttc aacatcttca cagccacttt
     3241 ggtcacacgg ttgggtttgt ccttgtccag cccgatagcc tctgccaaca ccacctgccc
     3301 aaagcagccc tctcccaggg gtttgcctaa gaccagtctg tcccgaggca gctcccagcg
     3361 agggtcttcg ggaagctcat actcagagac ccctgctagc atgggagtcc cactggagga
     3421 gagccgtgat ggccgaacca gaagaacccc agagttcatg gatgcactgg agtcagcaga
     3481 cactgttacc tgtctgcgca gagggatgct cttggccagc ttgtgcacag ccatctggct
     3541 gtggaagtca ctcttcttgg taccactctt catcttggaa ttcccagcac taccagcact
     3601 accagcacta tctgctcccg cgctgtcttt cccttttgat tttatacatc ccatggtggc
     3661 cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact
     3721 gttattcttt agaatggtgc aaagaataac ttcgtataat gtatgctata cgaagttatg
     3781 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     3841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4201 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     4261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4381 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     4441 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     4501 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     4561 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     4621 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     4681 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     4741 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     4801 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     4861 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
     4921 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4981 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     5041 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     5101 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     5161 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5221 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5281 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5341 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     5401 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5461 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     5521 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5581 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5881 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5941 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6001 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6061 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6121 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6181 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6241 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6301 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6361 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6421 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6481 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6541 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6601 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6661 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6721 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6781 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6841 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatg
//
