LOCUS       sequence-497740-        7356 bp    DNA     circular SYN 16-JAN-2026
DEFINITION  AAV expressing light-activatable FGFR1 (optoFGFR1-HA) under the 
            CaMKII-alpha promoter for excitatory-neuron-specific activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Shin J, Oh H, Park JH, Bae H, Yang CM, Lee M, Kim S, Heo WD
  TITLE     Dysregulation of FGFR1 signaling in the hippocampus facilitates 
            depressive disorder.
  JOURNAL   Exp Mol Med. 2025 Aug;57(8):1818-1836. doi: 
            10.1038/s12276-025-01519-9. Epub 2025 Aug 15.
  PUBMED    40813470
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s12276-025-01519-9"; journalName: "Exp Mol Med"; date: 
            "2025-08"; volume: "57"; issue: "8"; pages: "1818-1836"
COMMENT     Sequence Label: AAV-CaMKII-alpha-optoFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..7356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      540..549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3423..3449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3485..4073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3538..3558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3956..3967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4076..4092)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4077..4093)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4103..4579
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4302..4321)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4619..4759
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4619..4748
                     /label=AAV2 ITR
     rep_origin      4834..5289
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4921..4940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5131..5152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5306..5325)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5425..5447
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5485..5503)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5571..5675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5676..5744,5745..6536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5894..5913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6707..7295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7196..7215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacttg tggactaagt ttgttcacat ccccttctcc
      181 aaccccctca gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt
      241 cctgcagtat tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca
      301 ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg
      361 ccatggggac ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc
      421 tcagaagccc caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca
      481 ggcgagtggc ccctagttct gggggcagct ctagagccga tccccgggta ccggtgctag
      541 ccaccatggg atgtataaaa tcaaaaggga aagacagcgc gggagcagat agtgctggta
      601 gtgctggtag tgctgggaat tccaagatga agagtggtac caagaagagt gacttccaca
      661 gccagatggc tgtgcacaag ctggccaaga gcatccctct gcgcagacag gtaacagtgt
      721 ctgctgactc cagtgcatcc atgaactctg gggttcttct ggttcggcca tcacggctct
      781 cctccagtgg gactcccatg ctagcagggg tctctgagta tgagcttccc gaagaccctc
      841 gctgggagct gcctcgggac agactggtct taggcaaacc cctgggagag ggctgctttg
      901 ggcaggtggt gttggcagag gctatcgggc tggacaagga caaacccaac cgtgtgacca
      961 aagtggctgt gaagatgttg aagtcggacg caacagagaa agacttgtca gacctgatct
     1021 cagaaatgga gatgatgaag atgatcggga agcataagaa tatcatcaac ctgctggggg
     1081 cctgcacgca ggatggtccc ttgtatgtca tcgtggagta tgcctccaag ggcaacctgc
     1141 gggagtacct gcaggcccgg aggcccccag ggctggaata ctgctacaac cccagccaca
     1201 acccagagga gcagctctcc tccaaggacc tggtgtcctg cgcctaccag gtggcccgag
     1261 gcatggagta tctggcctcc aagaagtgca tacaccgaga cctggcagcc aggaatgtcc
     1321 tggtgacaga ggacaatgtg atgaagatag cagactttgg cctcgcacgg gacattcacc
     1381 acatcgacta ctataaaaag acaaccaacg gccgactgcc tgtgaagtgg atggcacccg
     1441 aggcattatt tgaccggatc tacacccacc agagtgatgt gtggtctttc ggggtgctcc
     1501 tgtgggagat cttcactctg ggcggctccc cataccccgg tgtgcctgtg gaggaacttt
     1561 tcaagctgct gaaggagggt caccgcatgg acaagcccag taactgcacc aacgagctgt
     1621 acatgatgat gcgggactgc tggcatgcag tgccctcaca gagacccacc ttcaagcagc
     1681 tggtggaaga cctggaccgc atcgtggcct tgacctccaa ccaggagtac ctggacctgt
     1741 ccatgcccct ggaccagtac tcccccagct ttcccgacac ccggagctct acgtgctcct
     1801 caggggagga ttccgtcttc tctcatgagc cgctgcccga ggagccctgc ctgccccgac
     1861 acccagccca gcttgccaat ggcggactca aacgccgcgc ggatcccatg aagatggaca
     1921 aaaagaccat cgtctggttt cggagagatt tgagaataga agataatccc gcgctcgccg
     1981 ccgcggccca cgagggttcc gtcttccccg ttttcatttg gtgtcctgaa gaagaaggcc
     2041 agttttatcc cggaagggcc tctaggtggt ggatgaagca aagtctggcc catcttagcc
     2101 agtcactgaa agcactgggc agtgatctta ccctgatcaa gacacacaat accatctctg
     2161 ccatcctcga ctgcatcagg gtgaccggcg caacgaaagt cgtgtttaac cacctgtacg
     2221 atccagttag tctggtgcgc gaccacactg tgaaggagaa gctggtggaa cgggggatca
     2281 gtgtgcagag ctacaacggg gaccttctgt acgagccatg ggagatctat tgcgagaaag
     2341 ggaaaccgtt cacctccttc aacagttact ggaagaaatg tttggatatg tcaatagagt
     2401 ccgttatgtt gccccctccc tggagactga tgccgattac tgctgctgca gaggccatct
     2461 gggcctgctc catcgaggaa ctcggtctgg aaaatgaagc agaaaagcca agcaatgcac
     2521 ttctcactag agcctggagc cccggctggt ctaatgccga caagctgctt aacgagttca
     2581 tcgaaaaaca actgattgac tacgcgaaga actccaagaa agtggtaggt aactcaacta
     2641 gcttgctctc tccatatctc cattttggcg agatttctgt ccgccatgta tttcagtgcg
     2701 ctcggatgaa acagattatc tgggctcgcg ataaaaacag cgaaggcgcc gaaagcgccg
     2761 atctgttcct gcgagggatc ggacttcggg aatactcccg gtatatatgt ttcaactttc
     2821 cattcacaca cgagcagagt ctgttgtccc acctcaggtt cttcccctgg gacgccgatg
     2881 tcgacaaatt caaggcatgg agacagggaa ggacagggta cccactcgtg gatgctggca
     2941 tgagagagct ctgggctaca ggctggatgc acaaccgcat ccgggtaatc gtgtcctcat
     3001 ttgctgtcaa gtttctgctc ctgccttgga aatggggaat gaagtacttt tgggataccc
     3061 ttctcgacgc cgacttggag tgtgacattc tgggatggca atatattagc gggtcaattc
     3121 ctgacggcca tgagttggac aggttggaca atccggcctt gcagggagct aagtatgatc
     3181 ccgaaggaga gtatattcga cagtggctcc ccgagctggc ccgacttcct acggagtgga
     3241 ttcaccatcc ttgggacgca ccactgacag tgctcaaggc aagcggggtg gagctgggca
     3301 ccaattacgc taagcctata gttgatatag atacagcacg cgagctgctg gctaaagcga
     3361 tctctcgcac tcgggaggcg cagattatga tcggtgctgc cggcagcggc agcccaccgg
     3421 tatatccata tgatgttcca gattatgctt aaggcgcgcc gaattcgata tcaagcttat
     3481 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3541 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3601 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3661 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3721 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3781 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3841 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3901 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3961 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4021 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     4081 ccgtcgacct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
     4141 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     4201 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     4261 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     4321 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     4381 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     4441 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     4501 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     4561 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
     4621 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4681 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4741 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4801 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4921 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5041 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5161 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     5221 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5281 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5341 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5401 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5461 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5521 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6841 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7321 ggttcctggc cttttgctgg ccttttgctc acatgt
//
