LOCUS       sequence-497771-       10976 bp    DNA     circular SYN 20-JAN-2026
DEFINITION  Empty vector (GFP only) control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 20, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2730 - pHR-EF1A-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2222..3399
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2453..3391
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3348..3368
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(3447..3471)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3503..4069
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3519..4071
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3663..3680)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3890..3909
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4083..4085,4086..4088,4089..4802)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4128..4149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4389..4408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4736..4757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4840..5428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4893..4913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5311..5322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5431..5447)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5432..5448)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5538..5771
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5796..5814)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5797..5814)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6104..6123)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6337..6356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6456..6478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6516..6534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6602..6706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6707..6775,6776..7567)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6925..6944)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7738..8326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8227..8246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8480..8497
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8567..8587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8572..8901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8752..8887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10035..10100
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10230..10250
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10675..10809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10712..10731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10766..10785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattctgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2281 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2341 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2401 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2461 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2521 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2581 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2641 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2701 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2761 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2821 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2881 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     2941 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3001 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3061 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3121 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3181 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3241 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3301 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3361 agtggttcaa agtttttttc ttccatttca ggtgtcgtgg aattctcacg cgtggatccg
     3421 cggccgcccc gggttaatta atctagaccc agctttcttg tacaaagtgg tgacgacggt
     3481 atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc gaagccgctt
     3541 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3601 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3661 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3721 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3781 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3841 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3901 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3961 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4021 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4141 acggcgacgt aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct
     4201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4261 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4561 acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4741 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4801 aacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     4861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5221 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     5281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5401 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     5461 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5521 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5581 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5641 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5701 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5761 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     5821 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     5881 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     5941 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     6001 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     6061 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     6121 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     6181 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     6241 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     6301 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     6361 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6421 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6481 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6541 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6781 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6841 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6901 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6961 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7021 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7081 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7141 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7201 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7261 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7321 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7381 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7441 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7501 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7561 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7621 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7681 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7741 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7801 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7861 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7921 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7981 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8101 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8281 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8341 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8401 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8461 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8521 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     8581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8881 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     8941 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     9001 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     9061 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     9121 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     9181 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     9241 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     9301 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     9361 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     9421 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     9481 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     9541 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     9601 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     9661 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     9721 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     9781 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     9841 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     9901 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     9961 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    10021 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    10081 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    10141 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    10201 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    10261 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    10321 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    10381 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    10441 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    10501 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    10561 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    10621 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    10681 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10741 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10801 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10861 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10921 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
