LOCUS       sequence-497831-        8922 bp    DNA     circular SYN 21-JAN-2026
DEFINITION  Expresses an actuator of Opto-p53 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: pPBbsr2-miRFP703-dSal-p53NT(1-97)-S15D-hCRY2-NLS
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1973..1995
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2057..2076
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2119..3063
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             4870..4890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4986..5535
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5130..5147)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5357..5376
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6006..6023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6012..6236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6283..6299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6307..6323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6331..6337,6338..6355,6356..6361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6376..6397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6631..6693
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6734..6754)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6734..6752)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6773..6789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6797..6813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6821..6827,6828..6845,6846..6851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6866..6887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7004..7021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7175..7763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7255..7274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7934..8725,8726..8794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8557..8576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8795..8899)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggggggcgc cggggggggg gggggggggg gggggggggg gggggggggg
     1561 gggggggggg gggggggggg ggcggggggg gggggggggg gggggggggg gggggggggg
     1621 gggggggggg ggggggggcg gggggggggg gggggggggg ggggggggcg cggcggcccc
     1681 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     1741 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     1801 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     1861 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1921 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1981 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     2041 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     2101 ttgggatccg aattcaccat ggtagcaggt catgcctctg gcagccccgc attcgggacc
     2161 gcctctcatt cgaattgcga acatgaagag atccacctcg ccggctcgat ccagccgcat
     2221 ggcgcgcttc tggtcgtcag cgaacatgat catcgcgtca tccaggccag cgccaacgcc
     2281 gcggaatttc tgaatctcgg aagcgtactc ggcgttccgc tcgccgagat cgacggcgat
     2341 ctgttgatca agatcctgcc gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg
     2401 cgctgccgga tcggcaatcc ctctacggag tactgcggtc tgatgcatcg gcctccggaa
     2461 ggcgggctga tcatcgaact cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg
     2521 gcgccggcgc tggagcggat ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc
     2581 gtgctgctgt ttcagcagtg caccggctac gaccgggtga tggtgtatcg tttcgatgag
     2641 caaggccacg gcctggtatt ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc
     2701 aaccgctatc cgtcgtcgct ggtcccgcag atggcgcggc agctgtacgt gcggcagcgc
     2761 gtccgcgtgc tggtggacgt cacctatcag ccggtgccgc tggagccgcg gctgtcgccg
     2821 ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc gctcgatgtc gccgatccat
     2881 ctgcagttcc tgaaggacat gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc
     2941 ggcaagctgt ggggcctggt tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag
     3001 ctgcgggcga tctgcaaacg gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag
     3061 agcctcgagg ccatggagga gccgcagtca gatcctagcg tcgagccccc tctggatcag
     3121 gaaacatttt cagacctatg gaaactactt cctgaaaaca acgttctgtc ccccttgccg
     3181 tcccaagcaa tggatgattt gatgctgtcc ccggacgata ttgaacaatg gttcactgaa
     3241 gacccaggtc cagatgaagc tcccagaatg ccagaggctg ctccccccgt ggcccctgca
     3301 ccagcagctc ctacaccggc ggcccctgca ccagccccct cctggcccct gtcatcttct
     3361 gtcggcggcc gcaagatgga caagaaaacc atcgtgtggt ttcggagaga tctgaggatt
     3421 gaggacaacc cagccctggc cgctgccgct cacgaaggca gcgtgtttcc agtgttcatc
     3481 tggtgccccg aggaagaggg acagttctac ccaggaaggg ccagccgctg gtggatgaag
     3541 cagtccctgg ctcacctgtc tcagagtctg aaggccctgg ggtcagatct gaccctgatc
     3601 aaaacccata acacaatcag cgccattctg gactgtattc gggtgactgg agccaccaaa
     3661 gtggtgttta atcacctgta tgatcccgtg agcctggtgc gggaccatac agtgaaggag
     3721 aaactggtgg aaagaggcat cagtgtgcag tcatacaacg gggatctgct gtatgagcca
     3781 tgggaaatct actgcgagaa ggggaaaccc tttaccagct tcaattccta ctggaagaaa
     3841 tgtctggaca tgtctatcga gagtgtgatg ctgccccctc catggaggct gatgcctatt
     3901 acagccgctg ccgaagctat ctgggcctgc tccattgaag agctgggact ggaaaacgag
     3961 gccgaaaagc cttctaatgc tctgctgact cgcgcctggt ccccaggatg gtctaacgct
     4021 gataaactgc tgaatgagtt catcgaaaag cagctgattg actatgccaa aaactctaag
     4081 aaagtggtgg gcaacagcac cagcctgctg tctccatacc tgcactttgg agagatcagt
     4141 gtgcggcacg tgttccagtg cgctagaatg aagcagatca tttgggccag ggataaaaac
     4201 agtgagggcg aagagtcagc cgacctgttt ctgaggggaa tcggactgag ggagtactcc
     4261 cgctatattt gtttcaattt tcccttcact cacgaacagt cactgctgag ccatctgcgg
     4321 ttctttcctt gggacgctga tgtggacaag ttcaaagcct ggcggcaggg aagaacaggc
     4381 tatcccctgg tggatgctgg aatgagagag ctgtgggcca ctggctggat gcacaatagg
     4441 atccgcgtga ttgtgagctc ctttgctgtg aagttcctgc tgctgccttg gaagtggggc
     4501 atgaaatatt tttgggatac actgctggat gccgacctgg agtgtgacat cctggggtgg
     4561 cagtacatct ccggatctat tccagatggc catgaactgg ataggctgga caaccccgct
     4621 ctgcagggcg ctaagtatga ccctgaggga gaatacatca ggcagtggct gcctgagctg
     4681 gctaggctgc caactgaatg gattcaccat ccctgggacg ctcctctgac agtgctgaag
     4741 gccagtgggg tggagctggg aactaattac gctaaaccta tcgtggatat tgacaccgcc
     4801 agagagctgc tggctaaggc catctcccgg accagagaag cccagatcat gattggcgct
     4861 gcctctagac ctaagaagaa gcgtaaggtg taaggatccg tcgacgggcc gcggtaacaa
     4921 ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc cgcccccccc
     4981 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5041 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5101 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5161 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5221 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5281 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5341 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5401 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5461 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5521 aaaaacacga taataccatg gtcatgaaaa catttaacat ttctcaacaa gatctagaat
     5581 tagtagaagt agcgacagag aagattacaa tgctttatga ggataataaa catcatgtgg
     5641 gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc agtacatatt gaagcgtata
     5701 taggacgagt aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac
     5761 aaaaggattt tgacacgatt gtagctgtta gacaccctta ttctgacgaa gtagatagaa
     5821 gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt gatttcagac tatgcaccag
     5881 attgttttgt gttaatagaa atgaatggca agttagtcaa aactacgatt gaagaactca
     5941 ttccactcaa atatacccga aattaaaagt cgagatgcat cgatgatcta gagctcgctg
     6001 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6061 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6121 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6181 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6241 tgaggcggaa agaacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     6301 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6361 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6421 tttccagtcg ggaaacctgt cgtgccagcg gatcttaaaa gttttgttac tttatagaag
     6481 aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat tgtttgttga
     6541 atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa
     6601 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat gattatcttt
     6661 aacgtacgtc acaatatgat tatctttcta gggttaatct agtatacgcg tttaattaat
     6721 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6781 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6841 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6901 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6961 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7021 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7081 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7141 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7201 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7261 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7321 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7381 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7441 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7501 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7561 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7621 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7681 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7801 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7861 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7921 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7981 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8041 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8101 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8161 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8221 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8281 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8341 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8401 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8461 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8521 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8581 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8641 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8701 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8761 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8821 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8881 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
