LOCUS       sequence-497832-        3480 bp    DNA     circular SYN 21-JAN-2026
DEFINITION  Magnetically modulated luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3480)
  TITLE     magnetogenetic luciferase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: M1_pTwistAmpHigh_T7_MagLuc
FEATURES             Location/Qualifiers
     source          1..3480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     86..105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        86..104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             137..157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             179..202
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1386..1402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1386..1402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1399..1421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1574..2162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1654..1673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2279..2303)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFVHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(3467..3480,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
       61 cgcctgacct actagtatac gaaattaata cgactcacta tagggagacc acaacggttt
      121 ccctctagaa ataattttgt ttaactttaa gaaggaggat atatccatgg gcagcagcca
      181 ccatcaccac catcatcatc acagcagcgg cggagaacaa aaactgatca gcgaagagga
      241 tctgatggtg tttacgcttg aggacttcgt cggtgattgg gaacaaacgg ctgcatacaa
      301 tttagatcaa gtgcttgagc aaggaggagt ctcttccctt ttgcaaaact tagcagtttc
      361 cgtgaccccc attcagcgca tcgtgcgctc aggcgagaat gctttaaaaa ttgatattca
      421 tgtgattatt ccgtacgaag gtcttagtgc agatcagatg gctcaaattg aagaggtgtt
      481 caaggtcgtt tatccagtgg atgaccatca tttcaaagtt atccttccct acgggacgtt
      541 ggtaattgat ggagttacac ctaatatgct gaattacttc ggacgccctt acgaaggaat
      601 cgcggttttc gatggcaaga agattaccgt cactggaacc ttatggaatg gcaataagat
      661 catcgacgag cgcttaatca cgcccgatgg tagcatgctg ttccgtgtta cgattaatag
      721 tggatccatg cttgctacta ctttagaacg tatagagaaa aacttcgtga tcacggaccc
      781 gagactacct gacaacccta taatttttgc aagtgactca ttccttcagt tgactgagta
      841 ttctagggaa gagattctag ggagaaatcc tagattcttg caaggaccag aaactgaccg
      901 tgccactgtg aggaaaatca gggatgcgat cgacaaccaa accgaggtga cagtgcagct
      961 aataaattac actaaatctg gcaagaagtt ctggaaccta tttcatgtgc aacccatgag
     1021 agaccaaaaa ggagacgtac agtacttcat aggggtaaag ttggatggta ctgagcatgt
     1081 tagagacgcg gcagaacgta ttaaggttat gttaataaat aagaccgctg ctaacataga
     1141 cgaagcggca aaggagttgg gggggagtgg aggaggctct ggaggagtaa ctggttatcg
     1201 tctgttcgaa gagattctgg gtaccggagg tcctactctc ctcaaggaag gcaaaaggac
     1261 ggggggcggc gtggaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     1321 tttctcgaga ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
     1381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
     1441 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
     1501 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2161 aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa gccgccgtcc
     2221 cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac caattcggag
     2281 cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgaacaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca tcgtacggcc
     3421 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
//
