LOCUS       sequence-49785-e       12794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Diao F, White BH
  TITLE     A novel approach for directing transgene expression in Drosophila: 
            T2A-Gal4 in-frame fusion.
  JOURNAL   Genetics. 2012 Mar;190(3):1139-44. Epub 2011 Dec 29.
  PUBMED    22209908
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2012-03"; volume: "190(3)"; pages: "1139-44. Epub 2011 Dec 
            29"
COMMENT     Sequence Label: pC-attB-burs-mCD8-EGFP-Gal4
FEATURES             Location/Qualifiers
     source          1..12794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..97
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(1237..1239,1240..1242,1243..1953)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1960..4605
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5098..5114)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5098..5114)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(5131..5332,5333..5363)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(5900..5919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6019..6041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6079..6097)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6165..6269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6270..6338,6339..7130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6488..6507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7301..7889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7790..7809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(8124..8154,8155..8709)
                     /label=P element 5' end
     primer_bind     12768..12786
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gaattcgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
       61 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
      121 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
      181 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cggatgcgga
      241 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg gcatgtcgac aggcctggta
      301 cctgattagc caataagttg tgagggaaat aaagagattg ccgtggtcgt gcgccgcggg
      361 agaaatgccc ttttcgtgta cgcaattact aggcaaaaag gtttggacgg cccttgcttt
      421 attaggccat aatttacgct gtcgagcggc cattagagcg gcatccgctg gccaagttgg
      481 tctataaaag cccggcggct gcaagtggcg ttttccattc cacgtgaaag gacactcgca
      541 gtcgggccga cgagaaaaat caaaatggcc agccccctga cccgcttcct gagcctgaac
      601 ctgctgctgc tgggcgagag catcatcctg ggcagcggcg aggccaagcc ccaggccccc
      661 gagctgcgca tcttccccaa gaagatggat gccgagctgg gccagaaggt ggatctggtg
      721 tgcgaggtgc tgggcagcgt gagccagggc tgcagctggc tgttccagaa cagcagcagc
      781 aagctgcccc agcccacctt cgtggtgtac atggccagca gccacaacaa gatcacctgg
      841 gatgagaagc tgaacagcag caagctgttc agcgccatgc gcgataccaa caacaagtac
      901 gtgctgaccc tgaacaagtt cagcaaggag aacgagggct actacttctg cagcgtgatc
      961 agcaacagcg tgatgtactt cagcagcgtg gtgcccgtgc tgcagaaggt gaacagcacc
     1021 accaccaagc ccgtgctgcg cacccccagc cccgtgcacc ccaccggcac cagccagccc
     1081 cagcgccccg aggattgccg cccccgcggc agcgtgaagg gcaccggcct ggatttcgcc
     1141 tgcgatatct acatctgggc acccctggcc ggcatctgcg tggccctgct gctgagcctg
     1201 atcatcaccc tgatctgcta ccacagccgc ggcagcatgg tgagcaaggg cgaggagctg
     1261 ttcaccggcg tggtgcccat cctggtggag ctggatggcg atgtgaacgg ccacaagttc
     1321 agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tgaccaccct gacctacggc
     1441 gtgcagtgct tcagccgcta ccccgatcac atgaagcagc acgatttctt caagagcgcc
     1501 atgcccgagg gctacgtgca ggagcgcacc atcttcttca aggatgatgg caactacaag
     1561 acccgcgccg aggtgaagtt cgagggcgat accctggtga accgcatcga gctgaagggc
     1621 atcgatttca aggaggatgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
     1681 cacaacgtgt acatcatggc cgataagcag aagaacggca tcaaggtgaa cttcaagatc
     1741 cgccacaaca tcgaggatgg cagcgtgcag ctggccgatc actaccagca gaacaccccc
     1801 atcggcgatg gccccgtgct gctgcccgat aaccactacc tgagcaccca gagcgccctg
     1861 agcaaggatc ccaacgagaa gcgcgatcac atggtgctgc tggagttcgt gaccgccgcc
     1921 ggcatcaccc tgggcatgga tgagctgtac aaggggccca tgaagctgct gagcagcatc
     1981 gagcaggcct gcgatatctg ccgcctgaag aagctgaagt gcagcaagga gaagcccaag
     2041 tgcgccaagt gcctgaagaa caactgggag tgccgctaca gccccaagac caagcgcagc
     2101 cccctgaccc gcgcccacct gaccgaggtg gagagccgcc tggagcgcct ggagcagctg
     2161 ttcctgctga tcttcccccg cgaggatctg gatatgatcc tgaagatgga tagcctgcag
     2221 gatatcaagg ccctgctgac cggcctgttc gtgcaggata acgtgaacaa ggatgccgtg
     2281 accgatcgcc tggccagcgt ggagaccgat atgcccctga ccctgcgcca gcaccgcatc
     2341 agcgccacca gcagcagcga ggagagcagc aacaagggcc agcgccagct gaccgtgagc
     2401 atcgatagcg ccgcccacca cgataacagc accatccccc tggatttcat gccccgcgat
     2461 gccctgcacg gcttcgattg gagcgaggag gatgatatga gcgatggcct gcccttcctg
     2521 aagaccgatc ccaacaacaa cggcttcttc ggcgatggca gcctgctgtg catcctgcgc
     2581 agcatcggct tcaagcccga gaactacacc aacagcaacg tgaaccgcct gcccaccatg
     2641 atcaccgatc gctacaccct ggccagccgc agcaccacca gccgcctgct gcagagctac
     2701 ctgaacaact tccaccccta ctgccccatc gtgcacagcc ccaccctgat gatgctgtac
     2761 aacaaccaga tcgagatcgc cagcaaggat cagtggcaga tcctgttcaa ctgcatcctg
     2821 gccatcggcg cctggtgcat cgagggcgag agcaccgata tcgatgtgtt ctactaccag
     2881 aacgccaaga gccacctgac cagcaaggtg ttcgagagcg gcagcatcat cctggtgacc
     2941 gccctgcacc tgctgagccg ctacacccag tggcgccaga agaccaacac cagctacaac
     3001 ttccacagct tcagcatccg catggccatc agcctgggcc tgaaccgcga tctgcccagc
     3061 agcttcagcg atagcagcat cctggagcag cgccgccgca tctggtggag cgtgtacagc
     3121 tgggagatcc agctgagcct gctgtacggc cgcagcatcc agctgagcca gaacaccatc
     3181 agcttcccca gcagcgtgga tgatgtgcag cgcaccacca ccggccccac catctaccac
     3241 ggcatcatcg agaccgcccg cctgctgcag gtgttcacca agatctacga gctggataag
     3301 accgtgaccg ccgagaagag ccccatctgc gccaagaagt gcctgatgat ctgcaacgag
     3361 atcgaggagg tgagccgcca ggcccccaag ttcctgcaga tggatatcag caccaccgcc
     3421 ctgaccaacc tgctgaagga gcacccctgg ctgagcttca cccgcttcga gctgaagtgg
     3481 aagcagctga gcctgatcat ctacgtgctg cgcgatttct tcaccaactt cacccagaag
     3541 aagagccagc tggagcagga tcagaacgat caccagagct acgaggtgaa gcgctgcagc
     3601 atcatgctga gcgatgccgc ccagcgcacc gtgatgagcg tgagcagcta catggataac
     3661 cacaacgtga ccccctactt cgcctggaac tgcagctact acctgttcaa cgccgtgctg
     3721 gtgcccatca agaccctgct gagcaacagc aagagcaacg ccgagaacaa cgagaccgcc
     3781 cagctgctgc agcagatcaa caccgtgctg atgctgctga agaagctggc caccttcaag
     3841 atccagacct gcgagaagta catccaggtg ctggaggagg tgtgcgcccc cttcctgctg
     3901 agccagtgcg ccatccccct gccccacatc agctacaaca acagcaacgg cagcgccatc
     3961 aagaacatcg tgggcagcgc caccatcgcc cagtacccca ccctgcccga ggagaacgtg
     4021 aacaacatca gcgtgaagta cgtgagcccc ggcagcgtgg gacccagccc cgtgcccctg
     4081 aagagcggcg ccagcttcag cgatctggtg aagctgctga gcaaccgccc ccccagccgc
     4141 aacagccccg tgaccatccc ccgcagcacc cccagccacc gcagcgtgac ccccttcctg
     4201 ggccagcagc agcagctgca gagcctggtg cccctgaccc ccagcgccct gttcggcggc
     4261 gccaacttca accagagcgg caacatcgcc gatagcagcc tgagcttcac cttcaccaac
     4321 agcagcaacg gccccaacct gatcaccacc cagaccaaca gccaggccct gagccagccc
     4381 atcgccagca gcaacgtgca cgataacttc atgaacaacg agatcaccgc cagcaagatc
     4441 gatgatggca acaacagcaa gcccctgagc cccggctgga ccgatcagac cgcctacaac
     4501 gccttcggca tcaccaccgg catgttcaac accaccacca tggatgatgt gtacaactac
     4561 ctgttcgatg atgaggatac cccccccaac cccaagaagg agtaaaatga atcgtagata
     4621 ctgaaaaacc ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat
     4681 ttatacatcg ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca
     4741 tgtaacctct gatctataga attttttaaa tgactagaat taatgcccat cttttttttg
     4801 gacctaaatt cttcatgaaa atatattacg agggcttatt cagaagctta tcgataccgt
     4861 cgactaaagc caaatagaaa ttattcagtt ctggcttaag tttttaaaag tgatattatt
     4921 tatttggttg taaccaacca aaagaatgta aataactaat acataattat gttagtttta
     4981 agttagcaac aaattgattt tagctatatt agctacttgg ttaataaata gaatatattt
     5041 atttaaagat aattcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttagat
     5101 ccactagttc tagagcggcc gcccaagctt tgcgtactcg caaattatta aaaataaaac
     5161 tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac ggacatgcta
     5221 agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac tcagaatact
     5281 attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt gccgacggga
     5341 ccaccttatg ttatttcatc atggtctggc cattctcatc gtgagcttcc gggtgctcgc
     5401 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     5461 ctgcttgttt ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa
     5521 aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta
     5581 tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta
     5641 cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat
     5701 tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca
     5761 cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg
     5821 ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggtgcggt atttcacacc
     5881 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5941 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6001 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6061 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6121 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6241 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6301 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6361 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6421 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6481 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6541 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6601 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6661 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6721 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6781 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6841 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6901 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6961 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7021 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7081 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7141 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7201 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7441 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7681 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7861 gctcgtcagg ggggcggagc ctatggaaaa acgccttctt cttgaactcg ggctcggtgc
     7921 cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc
     7981 tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca
     8041 cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc
     8101 cgctggacta cgtgggtctg gcccatgatg aaataacata aggtggtccc gtcgatagcc
     8161 gaagcttacc gaagtataca cttaaattca gtgcacgttt gcttgttgag aggaaaggtt
     8221 gtgtgcggac gaattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
     8281 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
     8341 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
     8401 aatttgtgat acccacttta aagattcgca gtggaaggct gcacctgcaa aaggtcagac
     8461 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
     8521 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
     8581 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
     8641 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
     8701 agcagcagcc gaattaattc tagttccagt gaaatccaag cattttctaa attaaatgta
     8761 ttcttattat tatagttgtt atttttgata tatataaaca acactattat gcccaccatt
     8821 tttttgagat gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta
     8881 acgctaatca ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta
     8941 gaataaatat agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat
     9001 acaaaaatcg cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt
     9061 gttgcggaga gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg
     9121 tgaattatga agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt
     9181 tttatgtagc gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt
     9241 agaagacaaa tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat
     9301 aggtcatatc ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat
     9361 aaacagtaaa cacgatggta ataatggtaa aaaaaaaaac aagcagttat ttcggatata
     9421 tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa
     9481 gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     9541 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     9601 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     9661 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     9721 gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga
     9781 ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag
     9841 agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg
     9901 ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggtaatt cggcaatcgt
     9961 tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac
    10021 tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt
    10081 aatatgcaaa tgtattctaa acaagactta catttatcgt ggcaaagacg ttttgaaagg
    10141 tcatgttggt caggaagagg aagatggctc cgttgatatt catcacgccc acttgcgtga
    10201 gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt
    10261 actcgcatct cattaatatt catatcttca acatgttcgc gagttaaatg aaatttattt
    10321 attttctgca aaactataaa ctatacatct cattgaaaaa aactaagaag ggtgtggaat
    10381 caggcaattc taactaaaat ctagcgaatt tgtttccaag aattgtaagc gttatatcat
    10441 ttgtttccac tggaaccact caccgttgtc tgaataagtc gcacttttac gaggagtggt
    10501 tccttgagca ccgacagcca ggatcgccac aggaccgccc ggaactgcat gaaccaggtg
    10561 gccttgtagg tgtacccatt ctccggctgc tccagtggct tctccaaatt tttggtggcc
    10621 aacaactgct ccatatcccg ggctactttg ctaatagcaa aattgtcgcc atatcttggc
    10681 gatccgatca cgggactcga tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa
    10741 gtccgccgga ttgtagttgg taggacactg ggcacccacg ctggatagga gttgagatgt
    10801 aatgtaatgc tagataccct taataaacac atcgaactca ctaggaaaag aagtcgacgg
    10861 cttcgctggg agtgcccaag aaagctaccc tgccctcggc catcagaagg atcttgtcaa
    10921 agagctcaaa cagctcggaa gacggctgat gaatggtcag gatgacggtc ttgcccttct
    10981 gcgacagctt cttcagcacc tggacgacgc tgtgggcggt aaatgagtcc agtccggagg
    11041 tgggctcatc gcagatcaga agcggcggat cggttagtgc ctcggaggcg aatgccagac
    11101 gcttcctttc tccgccggac agacctttca ccctgccggg cacaccgatg atcgtgtgct
    11161 gacatttgct gagcgaaagc tcctggatca cctgatccac gcgggccact cgctgccgat
    11221 aggtcagatg tcgtggcatc cgcaccatgg cctggaaaat caggtgttcc ctggccgtta
    11281 gggagccgat aaagaggtca tcctgctgga cataggcgca cctggcctgc atctccttgg
    11341 cgtccacagg ttggccattg agcagtcgca tcccggatgg cgatacttgg atgccctgcg
    11401 gcgatcgaaa ggcaagggca ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca
    11461 cggccaaaag ttcgcccgga taggccacgc cgcaaactga gtttcaaatt ggtaattgga
    11521 ccctttatta agatttcaca cagatcagcc gactgcgaat agaaactcac cgttcttgag
    11581 caaatgtttc ctgggcgccg gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt
    11641 gaccagctgc cgccatccgg agcccggctg attgaccgcc ccaaagatgt ccatattgtg
    11701 ccaggcatag gtgaggttct cggctagttg gccgctccct gaaccggagt cctccggcgg
    11761 actgggtggc aggagcgtgc cgtagttttt ggcctgcccg aagccctggt taatgcagct
    11821 ctgcgaagcg tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg
    11881 ttatgctcat ctaaccccga acaaaacgaa gtatcctacg aagtaggttt atacttttat
    11941 ttattttttg tgcatctagg atcagcttaa aatatctggt tgttatattt tttgtaaaaa
    12001 agaatgtagt cgaaaatgaa tgcctttaga tgtcttgatc atgatatgat cttaaaaatt
    12061 gtcttatata gcgagcacag ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc
    12121 gattgcggtt tgggattttt gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc
    12181 aatcgcagtt cctatagatc aagtgaactt aagatgtatg cacatgtact actcacattg
    12241 ttcagatgct cggcagatgg gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct
    12301 tggcccattg ccgggatttt tcacactttc ccctgcttac ccacccaaaa ccaatcacca
    12361 ccccaatcac tcaaaaaaca aacaaaaata agaagcgaga ggagttttgg cacagcactt
    12421 tgtgtttaat tgatggcgta aaccgcttgg agcttcgtca cgaaaccgct gacaaagtgc
    12481 aactgaaggc ggacattgac gctaggtaac gctacaaacg gtggcgaaag agatagcgga
    12541 cgcagcggcg aaagagacgg cgatatttct gtggacagag aaggaggcaa acagcgctga
    12601 ctttgagtgg aatgtcattt tgagtgagag gtaatcgaaa gaacctggta cttcaaatac
    12661 ccttggatcg aagtaaattt aaaactgatc agataagttc aatgatatcc agtgcagtaa
    12721 aaaataaaaa aaaaatatgt ttttttaaat ctacattctc caaaaaaggg ttttattaac
    12781 ttacatacat acta
//
