LOCUS       sequence-49787-e       12849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Diao F, White BH
  TITLE     A novel approach for directing transgene expression in Drosophila: 
            T2A-Gal4 in-frame fusion.
  JOURNAL   Genetics. 2012 Mar;190(3):1139-44. Epub 2011 Dec 29.
  PUBMED    22209908
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2012-03"; volume: "190(3)"; pages: "1139-44. Epub 2011 Dec 
            29"
COMMENT     Sequence Label: pC-attB-burs-mCD8-EGFP-T2A-Gal4
FEATURES             Location/Qualifiers
     source          1..12849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..97
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(1238..1240,1241..1243,1244..1954)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1961..2014
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2015..4660
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5153..5169)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5153..5169)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(5186..5387,5388..5418)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(5955..5974)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6074..6096
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6134..6152)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6220..6324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6325..6393,6394..7185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6543..6562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7356..7944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7845..7864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(8179..8209,8210..8764)
                     /label=P element 5' end
     primer_bind     12823..12841
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gaattcgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
       61 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
      121 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
      181 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cggatgcgga
      241 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga caggcctggt
      301 acctgattag ccaataagtt gtgagggaaa taaagagatt gccgtggtcg tgcgccgcgg
      361 gagaaatgcc cttttcgtgt acgcaattac taggcaaaaa ggtttggacg gcccttgctt
      421 tattaggcca taatttacgc tgtcgagcgg ccattagagc ggcatccgct ggccaagttg
      481 gtctataaaa gcccggcggc tgcaagtggc gttttccatt ccacgtgaaa ggacactcgc
      541 agtcgggccg acgagaaaaa tcaaaatggc cagccccctg acccgcttcc tgagcctgaa
      601 cctgctgctg ctgggcgaga gcatcatcct gggcagcggc gaggccaagc cccaggcccc
      661 cgagctgcgc atcttcccca agaagatgga tgccgagctg ggccagaagg tggatctggt
      721 gtgcgaggtg ctgggcagcg tgagccaggg ctgcagctgg ctgttccaga acagcagcag
      781 caagctgccc cagcccacct tcgtggtgta catggccagc agccacaaca agatcacctg
      841 ggatgagaag ctgaacagca gcaagctgtt cagcgccatg cgcgatacca acaacaagta
      901 cgtgctgacc ctgaacaagt tcagcaagga gaacgagggc tactacttct gcagcgtgat
      961 cagcaacagc gtgatgtact tcagcagcgt ggtgcccgtg ctgcagaagg tgaacagcac
     1021 caccaccaag cccgtgctgc gcacccccag ccccgtgcac cccaccggca ccagccagcc
     1081 ccagcgcccc gaggattgcc gcccccgcgg cagcgtgaag ggcaccggcc tggatttcgc
     1141 ctgcgatatc tacatctggg cacccctggc cggcatctgc gtggccctgc tgctgagcct
     1201 gatcatcacc ctgatctgct accacagccg cggcagcatg gtgagcaagg gcgaggagct
     1261 gttcaccggc gtggtgccca tcctggtgga gctggatggc gatgtgaacg gccacaagtt
     1321 cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg gtgaccaccc tgacctacgg
     1441 cgtgcagtgc ttcagccgct accccgatca catgaagcag cacgatttct tcaagagcgc
     1501 catgcccgag ggctacgtgc aggagcgcac catcttcttc aaggatgatg gcaactacaa
     1561 gacccgcgcc gaggtgaagt tcgagggcga taccctggtg aaccgcatcg agctgaaggg
     1621 catcgatttc aaggaggatg gcaacatcct gggccacaag ctggagtaca actacaacag
     1681 ccacaacgtg tacatcatgg ccgataagca gaagaacggc atcaaggtga acttcaagat
     1741 ccgccacaac atcgaggatg gcagcgtgca gctggccgat cactaccagc agaacacccc
     1801 catcggcgat ggccccgtgc tgctgcccga taaccactac ctgagcaccc agagcgccct
     1861 gagcaaggat cccaacgaga agcgcgatca catggtgctg ctggagttcg tgaccgccgc
     1921 cggcatcacc ctgggcatgg atgagctgta caaggggccc gagggccgcg gcagcctgct
     1981 gacctgcggc gatgtggagg agaaccccgg gcccatgaag ctgctgagca gcatcgagca
     2041 ggcctgcgat atctgccgcc tgaagaagct gaagtgcagc aaggagaagc ccaagtgcgc
     2101 caagtgcctg aagaacaact gggagtgccg ctacagcccc aagaccaagc gcagccccct
     2161 gacccgcgcc cacctgaccg aggtggagag ccgcctggag cgcctggagc agctgttcct
     2221 gctgatcttc ccccgcgagg atctggatat gatcctgaag atggatagcc tgcaggatat
     2281 caaggccctg ctgaccggcc tgttcgtgca ggataacgtg aacaaggatg ccgtgaccga
     2341 tcgcctggcc agcgtggaga ccgatatgcc cctgaccctg cgccagcacc gcatcagcgc
     2401 caccagcagc agcgaggaga gcagcaacaa gggccagcgc cagctgaccg tgagcatcga
     2461 tagcgccgcc caccacgata acagcaccat ccccctggat ttcatgcccc gcgatgccct
     2521 gcacggcttc gattggagcg aggaggatga tatgagcgat ggcctgccct tcctgaagac
     2581 cgatcccaac aacaacggct tcttcggcga tggcagcctg ctgtgcatcc tgcgcagcat
     2641 cggcttcaag cccgagaact acaccaacag caacgtgaac cgcctgccca ccatgatcac
     2701 cgatcgctac accctggcca gccgcagcac caccagccgc ctgctgcaga gctacctgaa
     2761 caacttccac ccctactgcc ccatcgtgca cagccccacc ctgatgatgc tgtacaacaa
     2821 ccagatcgag atcgccagca aggatcagtg gcagatcctg ttcaactgca tcctggccat
     2881 cggcgcctgg tgcatcgagg gcgagagcac cgatatcgat gtgttctact accagaacgc
     2941 caagagccac ctgaccagca aggtgttcga gagcggcagc atcatcctgg tgaccgccct
     3001 gcacctgctg agccgctaca cccagtggcg ccagaagacc aacaccagct acaacttcca
     3061 cagcttcagc atccgcatgg ccatcagcct gggcctgaac cgcgatctgc ccagcagctt
     3121 cagcgatagc agcatcctgg agcagcgccg ccgcatctgg tggagcgtgt acagctggga
     3181 gatccagctg agcctgctgt acggccgcag catccagctg agccagaaca ccatcagctt
     3241 ccccagcagc gtggatgatg tgcagcgcac caccaccggc cccaccatct accacggcat
     3301 catcgagacc gcccgcctgc tgcaggtgtt caccaagatc tacgagctgg ataagaccgt
     3361 gaccgccgag aagagcccca tctgcgccaa gaagtgcctg atgatctgca acgagatcga
     3421 ggaggtgagc cgccaggccc ccaagttcct gcagatggat atcagcacca ccgccctgac
     3481 caacctgctg aaggagcacc cctggctgag cttcacccgc ttcgagctga agtggaagca
     3541 gctgagcctg atcatctacg tgctgcgcga tttcttcacc aacttcaccc agaagaagag
     3601 ccagctggag caggatcaga acgatcacca gagctacgag gtgaagcgct gcagcatcat
     3661 gctgagcgat gccgcccagc gcaccgtgat gagcgtgagc agctacatgg ataaccacaa
     3721 cgtgaccccc tacttcgcct ggaactgcag ctactacctg ttcaacgccg tgctggtgcc
     3781 catcaagacc ctgctgagca acagcaagag caacgccgag aacaacgaga ccgcccagct
     3841 gctgcagcag atcaacaccg tgctgatgct gctgaagaag ctggccacct tcaagatcca
     3901 gacctgcgag aagtacatcc aggtgctgga ggaggtgtgc gcccccttcc tgctgagcca
     3961 gtgcgccatc cccctgcccc acatcagcta caacaacagc aacggcagcg ccatcaagaa
     4021 catcgtgggc agcgccacca tcgcccagta ccccaccctg cccgaggaga acgtgaacaa
     4081 catcagcgtg aagtacgtga gccccggcag cgtgggaccc agccccgtgc ccctgaagag
     4141 cggcgccagc ttcagcgatc tggtgaagct gctgagcaac cgccccccca gccgcaacag
     4201 ccccgtgacc atcccccgca gcacccccag ccaccgcagc gtgaccccct tcctgggcca
     4261 gcagcagcag ctgcagagcc tggtgcccct gacccccagc gccctgttcg gcggcgccaa
     4321 cttcaaccag agcggcaaca tcgccgatag cagcctgagc ttcaccttca ccaacagcag
     4381 caacggcccc aacctgatca ccacccagac caacagccag gccctgagcc agcccatcgc
     4441 cagcagcaac gtgcacgata acttcatgaa caacgagatc accgccagca agatcgatga
     4501 tggcaacaac agcaagcccc tgagccccgg ctggaccgat cagaccgcct acaacgcctt
     4561 cggcatcacc accggcatgt tcaacaccac caccatggat gatgtgtaca actacctgtt
     4621 cgatgatgag gatacccccc ccaaccccaa gaaggagtaa aatgaatcgt agatactgaa
     4681 aaaccccgca agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata
     4741 catcgttttg ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa
     4801 cctctgatct atagaatttt ttaaatgact agaattaatg cccatctttt ttttggacct
     4861 aaattcttca tgaaaatata ttacgagggc ttattcagaa gcttatcgat accgtcgact
     4921 aaagccaaat agaaattatt cagttctggc ttaagttttt aaaagtgata ttatttattt
     4981 ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta
     5041 gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata tatttattta
     5101 aagataattc gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact
     5161 agttctagag cggccgccca agctttgcgt actcgcaaat tattaaaaat aaaactttaa
     5221 aaataatttc gtctaattaa tattatgagt taattcaaac cccacggaca tgctaagggt
     5281 taatcaacaa tcatatcgct gtctcactca gactcaatac gacactcaga atactattcc
     5341 tttcactcgc acttattgca agcatacgtt aagtggatgt ctcttgccga cgggaccacc
     5401 ttatgttatt tcatcatggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
     5461 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
     5521 tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     5581 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     5641 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
     5701 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
     5761 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
     5821 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
     5881 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt gcggtatttc acaccgcata
     5941 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6001 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6061 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6121 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6181 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6241 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6301 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6361 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6421 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6481 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6541 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6601 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6661 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6721 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6781 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6841 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6901 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6961 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7021 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7081 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7141 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7201 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7501 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7741 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7801 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7861 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7921 tcaggggggc ggagcctatg gaaaaacgcc ttcttcttga actcgggctc ggtgccagta
     7981 tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt
     8041 aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc
     8101 cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg
     8161 gactacgtgg gtctggccca tgatgaaata acataaggtg gtcccgtcga tagccgaagc
     8221 ttaccgaagt atacacttaa attcagtgca cgtttgcttg ttgagaggaa aggttgtgtg
     8281 cggacgaatt tttttttgaa aacattaacc cttacgtgga ataaaaaaaa atgaaatatt
     8341 gcaaattttg ctgcaaagct gtgactggag taaaattaat tcacgtgccg aagtgtgcta
     8401 ttaagagaaa attgtgggag cagagccttg ggtgcagcct tggtgaaaac tcccaaattt
     8461 gtgataccca ctttaaagat tcgcagtgga aggctgcacc tgcaaaaggt cagacattta
     8521 aaaggaggcg actcaacgca gatgccgtac ctagtaaagt gatagagcct gaaccagaaa
     8581 agataaaaga aggctatacc agtgggagta cacaaacaga gtaagtttga atagtaaaaa
     8641 aaatcattta tgtaaacaat aacgtgactg tgcgttaggt cctgttcatt gtttaatgaa
     8701 aataagagct tgagggaaaa aattcgtact ttggagtacg aaatgcgtcg tttagagcag
     8761 cagccgaatt aattctagtt ccagtgaaat ccaagcattt tctaaattaa atgtattctt
     8821 attattatag ttgttatttt tgatatatat aaacaacact attatgccca ccattttttt
     8881 gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa ttgtaacgct
     8941 aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta aattagaata
     9001 aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg tacatacaaa
     9061 aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc
     9121 ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat
     9181 tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat
     9241 gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag
     9301 acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc
     9361 atatcttgtt tttattggca caaatataat tacattagct ttttgagggg gcaataaaca
     9421 gtaaacacga tggtaataat ggtaaaaaaa aaaacaagca gttatttcgg atatatgtcg
     9481 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
     9541 cgatgagaat ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg
     9601 tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt
     9661 cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc
     9721 atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagc
     9781 cgccaaagag caggaatggt atgataaccg gcggacccac agacagcgcc atcgaggtcg
     9841 aggagctggc gcaggatatt agatatccga aggacgttga cacattggcc accagagtga
     9901 ccagcgccag gcagttgaag aagtgcagca ctccggcccg cagtccgatc atcggatagg
     9961 caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg taattcggca atcgttttgc
    10021 ccagaaagta tgtgtcacag cgataaagtc gacttcgggc ctccctcata aaaactggca
    10081 gctctgaggt gaacacctaa atcgaatcga ttcattagaa agttagtaaa ttattaatat
    10141 gcaaatgtat tctaaacaag acttacattt atcgtggcaa agacgttttg aaaggtcatg
    10201 ttggtcagga agaggaagat ggctccgttg atattcatca cgcccacttg cgtgagttgt
    10261 tggcccaaaa agatgaggcc aatcaagatg gcaaccatct gcaaattaaa atgttactcg
    10321 catctcatta atattcatat cttcaacatg ttcgcgagtt aaatgaaatt tatttatttt
    10381 ctgcaaaact ataaactata catctcattg aaaaaaacta agaagggtgt ggaatcaggc
    10441 aattctaact aaaatctagc gaatttgttt ccaagaattg taagcgttat atcatttgtt
    10501 tccactggaa ccactcaccg ttgtctgaat aagtcgcact tttacgagga gtggttcctt
    10561 gagcaccgac agccaggatc gccacaggac cgcccggaac tgcatgaacc aggtggcctt
    10621 gtaggtgtac ccattctccg gctgctccag tggcttctcc aaatttttgg tggccaacaa
    10681 ctgctccata tcccgggcta ctttgctaat agcaaaattg tcgccatatc ttggcgatcc
    10741 gatcacggga ctcgatctcc cgtccgggca caacggccaa cacctgtacg taaaagtccg
    10801 ccggattgta gttggtagga cactgggcac ccacgctgga taggagttga gatgtaatgt
    10861 aatgctagat acccttaata aacacatcga actcactagg aaaagaagtc gacggcttcg
    10921 ctgggagtgc ccaagaaagc taccctgccc tcggccatca gaaggatctt gtcaaagagc
    10981 tcaaacagct cggaagacgg ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac
    11041 agcttcttca gcacctggac gacgctgtgg gcggtaaatg agtccagtcc ggaggtgggc
    11101 tcatcgcaga tcagaagcgg cggatcggtt agtgcctcgg aggcgaatgc cagacgcttc
    11161 ctttctccgc cggacagacc tttcaccctg ccgggcacac cgatgatcgt gtgctgacat
    11221 ttgctgagcg aaagctcctg gatcacctga tccacgcggg ccactcgctg ccgataggtc
    11281 agatgtcgtg gcatccgcac catggcctgg aaaatcaggt gttccctggc cgttagggag
    11341 ccgataaaga ggtcatcctg ctggacatag gcgcacctgg cctgcatctc cttggcgtcc
    11401 acaggttggc cattgagcag tcgcatcccg gatggcgata cttggatgcc ctgcggcgat
    11461 cgaaaggcaa gggcattcag cagggtcgtc tttccggcac cggaactgcc catcacggcc
    11521 aaaagttcgc ccggataggc cacgccgcaa actgagtttc aaattggtaa ttggaccctt
    11581 tattaagatt tcacacagat cagccgactg cgaatagaaa ctcaccgttc ttgagcaaat
    11641 gtttcctggg cgccggtatg tgtcgctcgt tgcagaatag tccgcgtgtc cggttgacca
    11701 gctgccgcca tccggagccc ggctgattga ccgccccaaa gatgtccata ttgtgccagg
    11761 cataggtgag gttctcggct agttggccgc tccctgaacc ggagtcctcc ggcggactgg
    11821 gtggcaggag cgtgccgtag tttttggcct gcccgaagcc ctggttaatg cagctctgcg
    11881 aagcgtccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac aagcgttatg
    11941 ctcatctaac cccgaacaaa acgaagtatc ctacgaagta ggtttatact tttatttatt
    12001 ttttgtgcat ctaggatcag cttaaaatat ctggttgtta tattttttgt aaaaaagaat
    12061 gtagtcgaaa atgaatgcct ttagatgtct tgatcatgat atgatcttaa aaattgtctt
    12121 atatagcgag cacagctacc agaataatct gtttcgtgtc actatttgtt tgtgcgattg
    12181 cggtttggga tttttgtggg tcgcagttct cacgccgcag acaatttgat gttgcaatcg
    12241 cagttcctat agatcaagtg aacttaagat gtatgcacat gtactactca cattgttcag
    12301 atgctcggca gatgggtgtt tgctgcctcc gcgaattaat agctcctgat cctcttggcc
    12361 cattgccggg atttttcaca ctttcccctg cttacccacc caaaaccaat caccacccca
    12421 atcactcaaa aaacaaacaa aaataagaag cgagaggagt tttggcacag cactttgtgt
    12481 ttaattgatg gcgtaaaccg cttggagctt cgtcacgaaa ccgctgacaa agtgcaactg
    12541 aaggcggaca ttgacgctag gtaacgctac aaacggtggc gaaagagata gcggacgcag
    12601 cggcgaaaga gacggcgata tttctgtgga cagagaagga ggcaaacagc gctgactttg
    12661 agtggaatgt cattttgagt gagaggtaat cgaaagaacc tggtacttca aatacccttg
    12721 gatcgaagta aatttaaaac tgatcagata agttcaatga tatccagtgc agtaaaaaat
    12781 aaaaaaaaaa tatgtttttt taaatctaca ttctccaaaa aagggtttta ttaacttaca
    12841 tacatacta
//
