LOCUS       sequence-497878-        7755 bp    DNA     circular SYN 21-JAN-2026
DEFINITION  Expresses tdTomato – Monodirectional chloroplast expression vector 
            (psbH Rescue) in Chlamydomonas Reinhardtii photosynthetically 
            deficient strain CC4388.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7755)
  TITLE     Engineering bidirectional chloroplast promoters for tunable 
            co-expression of multiple genes in microalgae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pATDt
FEATURES             Location/Qualifiers
     source          1..7755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18..40)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(234..253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     353..375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(413..431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        499..603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(604..672,673..1464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(822..841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2124..2143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2377..2394
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattat attagttgta aacttgacta acattttaaa ttttaaattt
     3241 tttcctaatt atatatttta cttgcaaaat ttataaaaat tttatgcatt tttatatcat
     3301 aataataaaa cctttattca tggtttataa tataataatt gtgatgacta tgcacaaagc
     3361 agttctagtc ccatatatat aactatatat aacccgttta aagatttatt taaaaatatg
     3421 tgtgtaaaaa atgcttattt ttaattttat tttatataag ttataatatt aaatacacaa
     3481 tgattaaaat taaataataa taaatttaac gtaacgatga gttgtttttt tattttggag
     3541 atacacgcaa tggtatctaa aggtgaagaa gtaattaagg aattcatgcg ttttaaagtt
     3601 cgtatggaag gtagtatgaa tggtcatgaa tttgaaattg aaggtgaggg tgaaggtcgt
     3661 ccatatgaag gtacacaaac tgctaagtta aaagtaacta aaggtggtcc attaccattt
     3721 gcatgggata ttttatcacc acaattcatg tatggttcaa aagcttatgt aaaacaccca
     3781 gctgatattc ctgactataa aaaattatca tttccagaag gttttaaatg ggaacgtgtt
     3841 atgaactttg aagatggtgg tttagtaaca gtaactcaag attctagttt acaagatggt
     3901 acattaatat ataaagttaa aatgcgtggt actaattttc cacctgatgg tccagtaatg
     3961 cagaaaaaga caatgggttg ggaagcttca actgaacgtt tatatccacg tgatggtgta
     4021 ctaaaaggtg aaattcatca agcacttaaa cttaaagatg gtggtcatta cttagtagaa
     4081 ttcaaaacaa tctatatggc taaaaaacct gtacaattac caggttatta ttatgttgat
     4141 actaaattag atattactag tcataatgaa gattatacaa ttgtagaaca atatgaacgt
     4201 tcagaaggtc gtcatcatct gtttttaggt catggtactg gatctacagg ttcaggtagt
     4261 agtggtacag cttcttctga agataataat atggctgtta ttaaggaatt catgcgtttt
     4321 aaagtaagaa tggaaggtag tatgaatggt catgaatttg aaattgaagg tgaaggtgaa
     4381 ggtagaccat atgaaggtac acaaactgct aagttaaaag ttacaaaagg tggtccatta
     4441 ccttttgctt gggatatttt aagtccacaa ttcatgtatg gttctaaagc ttatgttaaa
     4501 catccagctg atattccaga ttataaaaaa ttaagttttc ctgaaggttt taaatgggaa
     4561 cgtgtaatga attttgaaga tggtggttta gttacagtta cacaagacag ttctttacaa
     4621 gatggtactt taatctataa agttaaaatg agaggtacta attttccacc tgatggtcca
     4681 gtaatgcaaa agaaaacaat gggttgggaa gcaagtacag aacgtttata tcctcgtgat
     4741 ggtgttttaa aaggtgaaat tcaccaagct ttaaagttaa aagatggtgg tcactactta
     4801 gtagagttta aaacaatata tatggctaaa aaacctgtac aattaccagg ttactattat
     4861 gttgatacta aattagatat tacatcacat aatgaagatt atacaattgt tgaacaatat
     4921 gaacgtagtg aaggtcgtca tcacttattt ttatatggta tggatgaatt atataaataa
     4981 gtactcaagc tcgtaacgaa ggtcgtgacc ttgctcgtga aggtggcgac gtaattcgtt
     5041 cagcttgtaa atggtctcca gaacttgctg ctgcatgtga agtttggaaa gaaattaaat
     5101 tcgaatttga tactattgac aaactttaat ttttattttt catgatgttt atgtgaatag
     5161 cataaacatc gtttttattt ttatggtgtt taggttaaat acctaaacat cattttacat
     5221 ttttaaaatt aagttctaaa gttatctttt gtttaaattt gcctgtcttt ataaattacg
     5281 atgtgccaga aaaataaaat cttagctttt tattatagaa tttatcttta tgtattatat
     5341 tttataagtt ataataaaag aaatagtaac atactaaagc ggatgtagcg cgtttatctt
     5401 aacggaaggc cagtggcagt ggcggtgcca ctgccgaata taaatatggt tgagttgctt
     5461 agtttacctt agcgaaaaga agacttagca gctagcctta acaaacagtt ttatatttta
     5521 tgtttgtgtt aaataaaatt aagaaacttt agctaaagtt tcccaactca tagaaacgtc
     5581 atctaaaatt aaagaactgt tgtaaatttc taaaatgatt aataagaatg ctgcaaataa
     5641 aaggataaat acagccatta aaacagttgt accccagcct ggtaatactt tacctgcttc
     5701 tgagttaagt ggacgtaata aagtacctaa tggtgtaact aaaccaggtt cttggaagtc
     5761 tgaatttact tttgatggtt tagctttaga agttcctgtt gccataattg attaaatgaa
     5821 ttaagcgtta ttagcgctat tttatttact ttctgtaaaa aataaggaaa atattcttca
     5881 gtgcattccc tctcaggatt ataaatactc tgaggataac gttctctcgt caaggggttg
     5941 cttcttgtga gtatagaaac ctactagcac aagaaataaa ttgcataaaa atgtatttac
     6001 ctaggaccgc agtaggcagt cccttttccc cttcagaact gcctgcttta aaagaatgaa
     6061 aaaactgcct tgtctggtaa gtaaaactct ttaattactc actaaagacg atctagaagt
     6121 tctttgttca ttttttattt aatataatat ttgttatata aaaattaaat aatttttaat
     6181 taatgtttaa ctttgtaagg acagtttcaa agtgacatga atggctactg caaaaacgaa
     6241 gtaagttatt ctttctcagg gcaaaatttt gagtagatta attttgttta aaaatgtggg
     6301 acacagtcgt caagtctttt gaactatcta agagatatgt tgaaaagaga ataattttat
     6361 tattaaatga gctatggaaa gtccagcttt tttctttacc ttttttttat ggtttcttct
     6421 gttaagtgta actggctatt cagtttatgt tagttttggt ccaccttcaa aaaaattacg
     6481 tgatcctttt gaagaacacg aagattaaac aagttaaaaa gtactatttt tacaagtgac
     6541 ttcggtgcct ctgagaaccc tagttatagt gatataaaat aagtagctaa ctactttata
     6601 tttttatgaa agtcattttg tcgagcatat aaacaaaaac aaaattgcta tactaggcag
     6661 tcacagtgca actgtctccg tctccttaac cgagaaaggg taaacgtctt cggtaaagta
     6721 acaaacttta gttatgttaa ctcgcttgcg agttaaccat tttttttcct ccgaaggaca
     6781 acagttggca gttgccaaac tttagtggtc taatatttat attaggcagt tggcaggcaa
     6841 ctgccactga cgtcccgaag gggaaggggt ttacttacct cctaacggag tatataaata
     6901 gaataaaatt tatttcctgc gctagcagat ttacatacta ggattttaat actccgaagg
     6961 aggcagtggc ggtaccactg ccactggcgt cctccttccc cttcgggcaa atgcatttta
     7021 gtgccactta agtttacttg cctaggcagt tggcaggacg tcagtggcag tggtaccgcg
     7081 actgcctata tttatatact cctaagttta cttgcctagg cagttggcag gcaactgcca
     7141 ctgacgtcct tccccttccc cttcgggacg tccccttacg ggaatataaa tattagtgga
     7201 tatttatata ctgcgatgtt tacatactcc gaaggaggaa gcaggcagtt gcctgccaac
     7261 tgcctaatat aaatattggg caagtaaact tagaatgttt acatactccg aaggaggacg
     7321 tccccttacg ggaatataaa tattagtggc agtggtaccg ccactgcctc cttcggagta
     7381 ttaaaatcct agtatataaa tataccgtaa ggggacgtcc tccgacggtg gcagtggcgg
     7441 taccactgcc accggcgtcc taatatacat attgaagtat ttaaacctgt tagcgcagcc
     7501 tctaacgagt cagtaaactt cccttttggg gcttctaggc agcgcataaa ttttctagga
     7561 cgaacgtcca ctggcgtctc gtaaggagca gtgacaggcc actaatgtcc ccttaatggg
     7621 taaataaatg gctatcgtct atccatgaag agaccatata ttccagtagc accgttatga
     7681 tcctcaaagg gtaacaccat ttgtatagta ttatggtgaa atgcatccct ttcagggtag
     7741 atttatatct tacag
//
