LOCUS       sequence-497893-       11152 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  Expression of human mutant ETFDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Papadopoli D, Palia R, Jovanovic P, Tabaries S, Ciccolini E, 
            Sabourin V, Igelmann S, McLaughlan S, Zhan L, Kim H, Chekkal N, 
            Szkop KJ, Bertomeu T, Zeng J, Vassalakis J, Afzali F, Mzoughi S, 
            Guccione E, Tyers M, Avizonis D, Larsson O, Postovit L-M, Djuranovic
            S, Ursini-Siegel J, Siegel PM, Pollak M, Topisirovic I
  TITLE     Mitochondrial ETF insufficiency drives neoplastic growth by 
            selectively optimizing cancer bioenergetics
  JOURNAL   eLife, 2025 [Reviewed Preprint] 14:RP106587
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife, 2025
            [Reviewed Preprint] 14:RP106587"
COMMENT     Sequence Label: pLenti-hETFDH-Y304A_G306E
FEATURES             Location/Qualifiers
     source          1..11152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1116..1419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1420..1623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1574..1594
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1637..1817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1864..1989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2482..2715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2900..2944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3207..3322
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     protein_bind    3370..3390
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3391..4569
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3622..4560
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4517..4537
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4569..4593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6454..6478)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6496..7084
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6549..6569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6967..6978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7115..7614
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7150..7173)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7547..7566
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7631..8656
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7631..8656
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             8728..8961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9033..9154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9070..9089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9124..9143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9194..9329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9349..9368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9350..9368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9378..9395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9378..9394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9387..9409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9536..9991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9623..9642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9833..9854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10017..10121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10122..10190,10191..10982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10340..10359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agctttagtt attaatagta atcaattacg
     1081 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1141 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1201 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1261 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1321 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1381 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1441 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1501 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1561 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1621 gctggtttag tgaaccgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1681 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1741 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1801 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1861 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1921 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1981 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     2041 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     2101 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     2161 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     2221 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     2281 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     2341 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     2401 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     2461 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     2521 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     2581 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     2641 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     2701 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     2761 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2821 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2881 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2941 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     3001 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     3061 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     3121 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     3181 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     3241 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3301 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     3361 gaggctagcc aactttgtat agaaaagttg ggctccggtg cccgtcagtg ggcagagcgc
     3421 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     3481 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     3541 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     3601 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     3661 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     3721 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     3781 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     3841 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     3901 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     3961 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     4021 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     4081 tctcaagctg gccggcctgc tctggtgcct ggtctcgcgc cgccgtgtat cgccccgccc
     4141 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     4201 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     4261 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     4321 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     4381 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     4441 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     4501 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     4561 gtgtcgtgac aagtttgtac aaaaaagcag gctgccacca tgctggtgcc gctagccaag
     4621 ctgtcctgcc tggcatatca gtgctttcat gccttaaaaa ttaagaaaaa ttatctacct
     4681 ctatgtgcta caagatggtc ttcaacttct actgtgcctc gaattactac ccattatact
     4741 atttatcccc gggataagga caagagatgg gaaggagtga acatggaaag gtttgcagaa
     4801 gaagcagatg ttgtaatagt tggtgcaggc cctgcagggc tctctgcagc tgttcgtcta
     4861 aaacagttgg ctgtggcaca tgaaaaggac atccgtgtgt gtctagtgga gaaagctgcc
     4921 cagataggag ctcatactct ctcaggggct tgccttgatc caggtgcttt taaagaactc
     4981 ttcccagact ggaaagagaa gggggctcca cttaacactc ctgtaacaga agacagattt
     5041 ggaattttaa cagagaaata cagaattcct gtgccaattc ttccagggct tccaatgaat
     5101 aatcatggca attacattgt acgcttggga catttagtga gctggatggg cgaacaagca
     5161 gaagcccttg gtgttgaagt ataccctggt tatgcagctg ctgaggtcct ttttcatgat
     5221 gatggtagtg taaaaggaat tgccactaac gatgtaggga tacaaaagga tggtgcacca
     5281 aaggcaacat ttgagagagg actggaacta catgctaaag tcacaatttt tgcagaaggt
     5341 tgccatggac atctagccaa gcaactatat aagaagtttg atttgagagc aaattgtgaa
     5401 cctcaaacct acgggattgg actgaaggag ttatgggtta ttgatgaaaa gaactggaaa
     5461 cctgggagag tagatcacac tgttggttgg cccttggaca gacataccgc tggagaatct
     5521 ttcctctatc atttgaatga aggtgaaccc ctagtagctc ttggtcttgt ggttggtcta
     5581 gactatcaga atccatacct gagtccattt agagagttcc aaaggtggaa acaccatcct
     5641 agcattcggc caaccttgga aggtggaaaa aggattgcat acggagccag agctctcaat
     5701 gaaggtggct ttcagtctat accaaaactc acctttcctg gtggtttact aattggttgt
     5761 agtcctggtt ttatgaatgt tcccaagatc aaaggtactc acacagcaat gaaaagtgga
     5821 attttagcag cagaatctat ttttaatcaa ctaactagtg aaaatctcca atcaaagaca
     5881 ataggactcc atgtaactga atatgaggac aatttgaaga actcatgggt atggaaagag
     5941 ctatattctg ttagaaatat aagaccgtcc tgccacggag tactgggtgt atatggaggg
     6001 atgatttaca ctggaatctt ttactggata ttgagaggaa tggagccgtg gactctgaaa
     6061 cataaaggtt ctgactttga acggctcaag ccagccaagg attgcacacc tattgagtat
     6121 ccaaaacccg atggacagat cagttttgac ctcttgtcat ctgtggctct gagtggtact
     6181 aatcatgaac atgaccagcc ggcacactta accttaaggg atgacagtat acctgtaaat
     6241 agaaatctgt cgatatatga tgggcccgag cagcgattct gtcctgcagg agtttatgaa
     6301 tttgtacctg tggaacaagg tgatggattt cggttacaga taaatgctca gaactgtgta
     6361 cattgtaaaa catgtgatat taaagatcca agtcagaata ttaactgggt ggtacctgaa
     6421 ggtggaggag gacctgctta caatggaatg taaacccagc tttcttgtac aaagtggtga
     6481 taatcgaatt ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6541 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6601 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6661 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6721 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6781 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6841 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     6901 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6961 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7021 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7081 ccgcatcggg aattcccgcg gttcgaattc taccgggtag gggaggcgct tttcccaagg
     7141 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     7201 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     7261 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     7321 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     7381 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     7441 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     7501 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     7561 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctca
     7621 cgtggccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     7681 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     7741 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7801 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7861 gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7921 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7981 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     8041 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     8101 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     8161 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     8221 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     8281 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     8341 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     8401 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     8461 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     8521 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     8581 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     8641 gagggcaaag gaatagggta cctttaagac caatgactta caaggcagct gtagatctta
     8701 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8941 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     9001 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9301 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9361 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9601 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9661 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9721 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9781 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9841 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9901 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9961 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10021 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10081 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10141 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10201 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10261 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10321 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10381 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10441 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10501 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10561 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10621 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10681 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10741 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10801 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10861 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10921 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10981 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
