LOCUS       sequence-49795-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pTGMP
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(92..116)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(113..151)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(183..201,202..224,225..243,244..266,267..285,286..308,
                     309..327,328..350,351..369,370..392,393..411,412..434,
                     435..453)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(517..533)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(549..571)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctcgccctt gctcaccatg gtggtcgaga ggctggatcg gtcccggtgt cttctatgga
       61 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
      121 ttatataggc ctcccaccgt acacgcctac ctcgacccgg gtaccgagct cgactttcac
      181 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      241 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
      301 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
      361 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
      421 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgatgct ctagaggatc
      481 gatcccccag atctaattcc ggcgcctaga gaaggagtga gggctggata aagggaggat
      541 cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga
      601 ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa agccaaggct
      661 tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg tgaaaagacc
      721 ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg
      781 ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt gacatctacc
      841 gactg
//
