LOCUS       sequence-497969-       11549 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  SP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter 
            selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11549)
  TITLE     bioRxiv preprint: Genome-wide CRISPR knockout screening with viral 
            replicons for identification of host factors involved in viral 
            replication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CHIKV eGFP-Zeo replicon
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(6873..6875,6876..6878,6879..7589)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6873..6875,6876..6878,6879..7589)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6918..6939)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7179..7198)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7526..7547
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8640..8657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8750..9338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8830..8849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9509..10300,10301..10369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10132..10151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10370..10474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10775..10797
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10789..10806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10790..10806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10819..10837
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10819..10836
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gttcaacaga cctgacggaa ggtagacgag gcaagttgtc tattatgaga gggaaaaagc
       61 taaaaccgtg cgaccgtgtg ctgttctcag tagggtcaac gctctacccg gaaagccgca
      121 agctacttaa gagctggcac ctgccatcgg tgttccattt aaagggcaaa ctcagcttca
      181 catgccgctg tgatacagtg gtttcgtgtg agggctacgt cgttaagaga ataacgatga
      241 gcccaggcct ttatggaaaa accacagggt atgcggtaac ccaccacgca gacggattcc
      301 tgatgtgcaa gactaccgac acggttgacg gcgaaagaat gtcattctcg gtgtgcacat
      361 acgtgccggc gaccatttgt gatcaaatga ccggcatcct tgctacagaa gtcacgccgg
      421 aggatgcaca gaagctgttg gtggggctga accagagaat agtggttaac ggcagaacgc
      481 aacggaatac gaacaccatg aaaaattatc tgcttcccgt ggtcgcccaa gccctcagta
      541 agtgggcaaa ggagtgccgg aaagacatgg aagatgaaaa actcctgggg gtcagagaaa
      601 gaacactgac ctgctgctgt ctatgggcat tcaagaagca gaaaacacac acggtctaca
      661 agaggcctga tacccagtca attcagaagg ttcaggccga gtttgacagc tttgtggtac
      721 cgagtctgtg gtcgtccggg ttgtcaatcc ctttgaggac tagaatcaaa tggttgttaa
      781 gcaaggtgcc aaaaaccgac ctgatcccat acagcggaga cgcccgagaa gcccgggacg
      841 cagaaaaaga agcagaggaa gaacgagaag cagaactgac tcgcgaagcc ctaccacctc
      901 tacaggcagc acaggaagat gttcaggtcg aaatcgacgt ggaacagctt gaggacagag
      961 cgggcgcagg aataatagag actccgagag gagctatcaa agttactgcc caaccaacag
     1021 accacgtcgt gggagagtac ctggtactct ccccgcagac cgtactacgt agccagaagc
     1081 tcagtctgat tcacgctttg gcggagcaag tgaagacgtg cacgcacaac ggacgagcag
     1141 ggaggtatgc ggtcgaagcg tacgacggcc gagtcctagt gccctcaggc tatgcaatct
     1201 cgcctgaaga cttccagagt ctaagcgaaa gcgcaacgat ggtgtataac gaaagagagt
     1261 tcgtaaacag aaagctacac catattgcga tgcacggacc agccctgaac accgacgaag
     1321 agtcgtatga gctggtgagg gcagagagga cagaacacga gtacgtctac gacgtggatc
     1381 agagaagatg ctgtaagaag gaagaagccg caggactggt actggtgggc gacttgacta
     1441 atccgcccta ccacgaattc gcatatgaag ggctaaaaat ccgccctgcc tgcccataca
     1501 aaattgcagt cataggagtc ttcggagtac cgggatctgg caagtcagct attatcaaga
     1561 acctagttac caggcaggac ctggtgacta gcggaaagaa agaaaactgc caagaaatca
     1621 ccaccgacgt gatgagacag agaggtctag agatatctgc acgtacggtt gactcgctgc
     1681 tcttgaatgg atgcaacaga ccagtcgacg tgttgtacgt agacgaggcg tttgcgtgcc
     1741 actctggaac gctacttgct ttgatcgcct tggtgagacc aaggcagaaa gttgtacttt
     1801 gtggtgaccc gaagcagtgc ggcttcttca atatgatgca gatgaaagtc aactataatc
     1861 acaacatctg cacccaagtg taccacaaaa gtatctccag gcggtgtaca ctgcctgtga
     1921 ccgccattgt gtcatcgttg cattacgaag gcaaaatgcg cactacgaat gagtacaaca
     1981 agccgattgt agtggacact acaggctcaa caaaacctga ccctggagac ctcgtgttaa
     2041 cgtgcttcag agggtgggtt aaacaactgc aaattgacta tcgtggatac gaggtcatga
     2101 cagcagccgc atcccaaggg ttaaccagaa aaggagttta cgcagttaga caaaaagtta
     2161 atgaaaaccc gctctatgca tcaacgtcag agcacgtcaa cgtactccta acgcgtacgg
     2221 aaggtaaact ggtatggaag acactttccg gcgacccgtg gataaagacg ctgcagaacc
     2281 caccgaaagg aaacttcaaa gcaactatta aggagtggga ggtggagcat gcatcaataa
     2341 tggcgggcat ctgcagtcac caaatgacct tcgatacatt ccaaaataaa gccaacgttt
     2401 gttgggctaa gagcttggtc cctatcctcg aaacagcggg gataaaacta aatgataggc
     2461 agtggtctca gataattcaa gccttcaaag aagacaaagc atactcacct gaagtagccc
     2521 tgaatgaaat atgtacgcgc atgtatgggg tggatctaga cagcgggcta ttttctaaac
     2581 cgttggtgtc tgtgtattac gcggataacc actgggataa taggcctgga gggaaaatgt
     2641 tcggatttaa ccccgaggca gcatccattc tagaaagaaa gtatccattc acaaaaggga
     2701 agtggaacat caacaagcag atctgcgtga ctaccaggag gatagaagac tttaacccta
     2761 ccaccaacat cataccggcc aacaggagac taccacactc attagtggcc gaacaccgcc
     2821 cagtaaaagg ggaaagaatg gaatggctgg ttaacaagat aaacggccac cacgtgctcc
     2881 tggtcagtgg ctataacctt gcactgcctg gggaggaagg gagtactaag agagtcactt
     2941 gggtagcgcc gttaggtgtc cgcggagcgg actacacata caacctagag ttgggtctgc
     3001 cagcaacgct tggtaggtat gacctagtgg tcataaacat ccacacacct tttcgcatac
     3061 accattacca acagtgcgtc gaccacgcaa tgaaactgca aatgctcggg ggtgactcat
     3121 tgagactgct caaagggggc ggatccctat tgatcagagc atatggttac gcagatagaa
     3181 ccagtgaacg agtcatctgc gtattgggac gcaagtttag atcgtctaga gcgttgaaac
     3241 caccatgtgt caccagcaac actgagatgt ttttcctatt cagcaacttt gacaatggca
     3301 gaaggaattt cacaactcat gtcatgaaca atcaactgaa tgcagccttc gtaggacagg
     3361 tcacccgagc aggatgtgca ccgtcgtacc gggtaaaacg catggacatc gcgaagaacg
     3421 atgaagagtg cgtagtcaac gccgctaacc ctcgcgggtt accgggtggc ggtgtttgca
     3481 aggcagtata caaaaaatgg ccggagtcct ttaagaacag tgcaacacca gtgggaaccg
     3541 caaaaacagt tatgtgcggt acgtatccag taatccacgc tgttggacca aacttctcta
     3601 attattcgga gtctgaaggg gaccgggaat tggcagctgc ctatcgagaa gtcgcaaagg
     3661 aagtaactag gctgggagta aatagtgtag ctatacctct cctctccaca ggtgtatact
     3721 caggagggaa agacaggctg acccagtcac tgaaccacct ctttacagcc atggactcga
     3781 cggatgcaga cgtggtcatc tactgccgcg acaaagaatg ggagaagaaa atatctgagg
     3841 ccatacagat gcggacccaa gtagagctgc tggatgagca catctccata gactgcgatc
     3901 ttgttcgcgt gcaccctgac agcagcttgg caggcagaaa aggatacagc accacggaag
     3961 gcgcactgta ctcatatcta gaagggaccc gttttcatca gacggctgtg gatatggcgg
     4021 agatacatac tatgtggcca aagcaaacag aggccaatga gcaagtctgc ctatatgccc
     4081 tgggggaaag tattgaatcg atcaggcaga aatgcccggt ggatgatgca gacgcatcat
     4141 ctccccccaa aactgtccca tgcctttgcc gttacgctat gactccagaa cgcgtcaccc
     4201 ggcttcgcat gaaccacgtc acaagcataa ttgtgtgttc ttcgtttccc ctcccaaagt
     4261 acaaaataga aggagtgcaa aaagtcaaat gctctaaggt aatgctattt gaccacaacg
     4321 tgccatcgcg cgtaagtcca agggaatata gatcttccca ggagtctgca caggaggcga
     4381 gtacaatcac gtcactgacg catagtcaat tcgacctaag cgttgatggc gagatactgc
     4441 ccgtcccgtc agacctggat gctgacgccc cagccctaga accagcacta gacgacgggg
     4501 cgacacacac gctgccatcc acaaccggaa accttgcggc cgtgtctgat tgggtaatga
     4561 gcaccgtacc tgtcgcgccg cccagaagaa ggcgagggag aaacctgact gtgacatgtg
     4621 acgagagaga agggaatata acacccatgg ctagcgtccg attctttagg gcagagctgt
     4681 gtccggtcgt acaagaaaca gcggagacgc gtgacacagc aatgtctctt caggcaccac
     4741 cgagtaccgc cacggaaccg aatcatccgc cgatctcctt cggagcatca agcgagacgt
     4801 tccccattac atttggggac ttcaacgaag gagaaatcga aagcttgtct tctgagctac
     4861 taactttcgg agacttctta ccaggagaag tggatgactt gacagacagc gactggtcca
     4921 cgtgctcaga cacggacgac gagttaagac tagacagggc aggtgggtat atattctcgt
     4981 cggacaccgg tccaggtcat ttacaacaga agtcagtacg ccagtcagtg ctgccggtga
     5041 acaccctgga ggaagtccac gaggagaagt gttacccacc taagctggat gaagcaaagg
     5101 agcaactatt acttaagaaa ctccaggaga gtgcatccat ggccaacaga agcaggtatc
     5161 agtcgcgcaa agtagaaaac atgaaagcag caatcatcca gagactaaag agaggctgta
     5221 gactatactt aatgtcagag accccaaaag tccctactta ccggactaca tatccggcgc
     5281 ctgtgtactc gcctccgatc aacgtccgat tgtccaatcc cgagtccgca gtggcagcat
     5341 gcaatgagtt cttagctaga aactatccaa ctgtctcatc ataccaaatt accgacgagt
     5401 atgatgcata tctagacatg gtggacgggt cggagagttg cctggaccga gcgacattca
     5461 atccgtcaaa actcaggagc tacccgaaac agcacgctta ccacgcgccc tccatcagaa
     5521 gcgctgtacc gtccccattc cagaacacac tacagaatgt actggcagca gccacgaaaa
     5581 gaaactgcaa cgtcacacag atgagggaat tacccacttt ggactcagca gtattcaacg
     5641 tggagtgttt caaaaaattc gcatgcaacc aagaatactg ggaagaattt gctgccagcc
     5701 ctattaggat aacaactgag aatttagcaa cctatgttac taaactaaaa gggccaaaag
     5761 cagcagcgct attcgcaaaa acccataatc tactgccact acaggaagta ccaatggata
     5821 ggttcacagt agatatgaaa agggacgtaa aggtgactcc tggtacaaag catacagagg
     5881 aaagacctaa ggtgcaggtt atacaggcgg ctgaaccctt ggcgacagca tacctatgtg
     5941 ggattcacag agagctggtt aggaggctga acgccgtcct cctacccaat gtacatacac
     6001 tatttgacat gtctgccgag gatttcgatg ccatcatagc cgcacacttt aagccaggag
     6061 acactgtttt ggaaacggac atagcctcct ttgataagag ccaagatgat tcacttgcgc
     6121 ttactgcttt gatgctgtta gaggatttag gggtggatca ctccctgctg gacttgatag
     6181 aggctgcttt cggagagatt tccagctgtc acctaccgac aggtacgcgc ttcaagttcg
     6241 gcgccatgat gaaatcaggt atgttcctaa ctctgttcgt caacacattg ttaaacatca
     6301 ccatcgccag ccgagtgctg gaagatcgtc tgacaaaatc cgcgtgcgcg gccttcatcg
     6361 gcgacgacaa cataatacat ggagtcgtct ccgatgaatt gatggcagcc agatgtgcca
     6421 cttggatgaa catggaagtg aagatcatag atgcagttgt atccttgaaa gccccttact
     6481 tttgtggagg gtttatactg cacgatactg tgacaggaac agcttgcaga gtggcagacc
     6541 cgctaaaaag gctttttaaa ctgggcaaac cgctagcggc aggtgacgaa caagatgaag
     6601 atagaagacg agcgctggct gacgaagtga tcagatggca acgaacaggg ctaattgatg
     6661 agctggagaa agcggtatac tctaggtacg aagtgcaggg tatatcagtt gtggtaatgt
     6721 ccatggccac ctttgcaagc tccagatcca acttcgagaa gctcagagga cccgtcataa
     6781 ctttgtacgg cggtcctaaa taggtacgca ctacagctac ctattttgca gaagccgaca
     6841 gcaagtatct aaacactaat cagctaccta ggatggtgag caagggcgag gagctgttca
     6901 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6961 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     7021 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     7081 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     7141 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     7201 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     7261 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     7321 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     7381 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     7441 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     7501 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     7561 tcactctcgg catggacgag ctgtacaagg gtagcggcag cggtagcggt agcggcagca
     7621 tggctaaatt aacatctgcc gttcctgttt taacagctag ggatgttgca ggtgctgtag
     7681 agttttggac agataggtta ggattctcaa gagactttgt tgaggacgat tttgctggtg
     7741 ttgtcaggga tgacgttact ttatttatct cagcagtcca agatcaagtt gtccctgata
     7801 atacattggc ttgggtctgg gtcaggggtt tagatgaatt atatgctgaa tggtcagaag
     7861 ttgtatctac aaacttcaga gatgcttctg gtccagctat gaccgagatt ggtgaacagc
     7921 catggggtag agaatttgct ttgagagatc cagctggaaa ttgtgttcat tttgttgctg
     7981 aagaacaaga ttaagtttaa actaacttga caattaagta tgaaggtata tgtgtcccct
     8041 aagagacaca ctgtacatag caaataatct atagatcaaa gggctacgca acccctgaat
     8101 agtaacaaaa tacaaaatca ctaaaaatta taaaaacaga aaaatacata aataggtata
     8161 cgtgtcccct aagagacaca ttgtatgtag gtgataagta tagatcaaag ggccgaataa
     8221 cccctgaata gtaacaaaat atgaaaatca ataaaaatca taaaatagaa aaaccataaa
     8281 cagaagtagt tcaaagggct ataaaacccc tgaatagtaa caaaacataa aattaataaa
     8341 aatcaaatga ataccataat tggcaaacgg aagagatgta ggtacttaag cttcctaaaa
     8401 gcagccgaac tcactttgag aagtaggcat agcataccga actcttccac gattctccga
     8461 acccacaggg acgtaggaga tgttattttg tttttaatat ttcaaaaaaa aaaaaaaaaa
     8521 aaaaaaaaaa aaagcggccg cttccgctca ctgcccgctt tccagtcggg aaacctgtcg
     8581 tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc
     8641 tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa
     8701 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8761 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8821 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8881 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8941 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9001 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9061 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9121 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9181 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9241 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9301 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9361 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9421 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9481 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct
     9661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9721 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9781 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10141 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10381 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10441 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
    10501 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
    10561 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
    10621 agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc aactgttggg
    10681 aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10741 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10801 gccagtgatt taaatacgat ttaggtgaca ctatagatgg ctgcgtgaga cacacgtagc
    10861 ctaccagttt cttactgctc tactctgcaa agcaagagat taataaccca tcatggatcc
    10921 tgtgtacgtg gacatagacg ctgacagcgc ctttttgaag gccctgcaac gtgcgtaccc
    10981 catgtttgag gtggaaccaa ggcaggtcac accgaatgac catgctaatg ctagagcgtt
    11041 ctcgcatcta gctataaaac taatagagca ggaaattgac cccgactcaa ccatcctgga
    11101 tatcggcagt gcgccagcaa ggaggatgat gtcggacagg aagtaccact gcgtctgccc
    11161 gatgcgcagt gcggaagatc ccgagagact cgccaattat gcgagaaagc tagcatctgc
    11221 cgcaggaaaa gtcctggaca gaaacatctc tggaaagatc ggggacttac aagcagtaat
    11281 ggccgtgcca gacacggaga cgccaacatt ctgcttacac acagacgtct catgtagaca
    11341 gagagcagac gtcgctatat accaagacgt ctatgctgta cacgcaccca cgtcgctata
    11401 ccaccaggcg attaaagggg tccgagtggc gtactgggtt gggttcgaca caaccccgtt
    11461 catgtacaat gccatggcgg gtgcctaccc ctcatactcg acaaactggg cagatgagca
    11521 ggtactgaag gctaagaaca taggattat
//
