LOCUS       sequence-497975-        9103 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  Bidirectional chloroplast promoter (BDP3) enabling co-expression for
            transformation of Chlamydomonas reinhardtii photosynthetically 
            deficient strain CC4388.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  TITLE     Engineering bidirectional chloroplast promoters for tunable 
            co-expression of multiple genes in microalgae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pABDP3
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18..40)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(234..253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     353..375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(413..431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        499..603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(604..672,673..1464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(822..841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2124..2143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2377..2394
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcccct
     3241 tacgggaata taaatattag tggcagtggt accgccactg cctagtatat aaatatcggc
     3301 agttggcagg atatttatat actccgaagg aacttgttag ccgataggcg aggcaactgc
     3361 cactaaaatt tatttgcctc ctaacggagc attaaaatcc ctaagtttac ttgcccgtaa
     3421 ggggaagggg acgtccacta atatttatat taggcagttg gcaggcaaca ataaatacat
     3481 ttgtcccgta aggggacgtc ctgccaactg cctatggtag ctattaagta tatatatatg
     3541 aaaagtgtgt ataaactaaa ataaaccagg tatggttaac cagatttatt ttagtttaaa
     3601 aaaaaattta tttatataat tcatccatac ctaatgtaat accagcagct gttacaaatt
     3661 ctaataaaac catatgatca cgtttttcat ttggatcttt tgataattta gattgataac
     3721 taagataatg attatcaggt aataaaacag gaccatctcc aattggtgta ttttgttgat
     3781 aatgatcagc taattgaaca ccaccatctt caatattatg acgaatttta aaattagctt
     3841 tgataccatt tttctgttta tcagctgtaa tataaacatt atgtgaatta taattatatt
     3901 ctaatttatg acctaaaata ttaccatctt ctttaaaatc aataccttta agttcaatac
     3961 gattaactaa tgtatctcct tcaaatttaa cttcagcacg tgttttataa ttaccatcat
     4021 ctttaaagaa aattgtacgt tcttgtacat aaccttcagg cattgcactt ttaaagaaat
     4081 catgttgttt catgtgatct ggataacgag caaaacattg taaaccataa cctaatgtag
     4141 taactaaagt tggccatgga actggtaatt tacctgttgt acaaattaat tttaaagtta
     4201 atttaccata agttgcgtca ccttcacctt caccactaac tgaaaatttg tgaccattaa
     4261 cgtcaccatc taattcaact aaaattggaa ctacaccagt gaataattct tcaccttttg
     4321 aaaccattaa ttgattaaat gaattaagcg ttattagcgc tattttattt actttctgta
     4381 aaaaataagg aaaatattct tcagtgcatt ccctctcagg attataaata ctctgaggat
     4441 aacgttctct cgtcaagggg ttgcttcttg tgagtataga aacctactag cacaagaaat
     4501 aaattgcata aaaatgtatt tacctaggac cgcagtaggc agtccctttt ccccttcaga
     4561 actgcctgct ttaaaagaat gaaaaaactg ccttgtctgg taagtaaaac tctttaatta
     4621 ctcactaaag acgatctaga agttctttgt tcatttttta tttaatataa tatttgttat
     4681 ataaaaatta aataattttt aattaatgtt taactttgta aggacagttt caaagtgaca
     4741 tgaatggcta ctgcaaaaac gaagtaagtt attctttctc agggcaaaat tttgagtaga
     4801 ttaattttgt ttaaaaatgt gggacacagt cgtcaagtct tttgaactat ctaagagata
     4861 tgttgaaaag agaataattt tattattaaa tgagctaatg gtatctaaag gtgaagaagt
     4921 aattaaggaa ttcatgcgtt ttaaagttcg tatggaaggt agtatgaatg gtcatgaatt
     4981 tgaaattgaa ggtgagggtg aaggtcgtcc atatgaaggt acacaaactg ctaagttaaa
     5041 agtaactaaa ggtggtccat taccatttgc atgggatatt ttatcaccac aattcatgta
     5101 tggttcaaaa gcttatgtaa aacacccagc tgatattcct gactataaaa aattatcatt
     5161 tccagaaggt tttaaatggg aacgtgttat gaactttgaa gatggtggtt tagtaacagt
     5221 aactcaagat tctagtttac aagatggtac attaatatat aaagttaaaa tgcgtggtac
     5281 taattttcca cctgatggtc cagtaatgca gaaaaagaca atgggttggg aagcttcaac
     5341 tgaacgttta tatccacgtg atggtgtact aaaaggtgaa attcatcaag cacttaaact
     5401 taaagatggt ggtcattact tagtagaatt caaaacaatc tatatggcta aaaaacctgt
     5461 acaattacca ggttattatt atgttgatac taaattagat attactagtc ataatgaaga
     5521 ttatacaatt gtagaacaat atgaacgttc agaaggtcgt catcatctgt ttttaggtca
     5581 tggtactgga tctacaggtt caggtagtag tggtacagct tcttctgaag ataataatat
     5641 ggctgttatt aaggaattca tgcgttttaa agtaagaatg gaaggtagta tgaatggtca
     5701 tgaatttgaa attgaaggtg aaggtgaagg tagaccatat gaaggtacac aaactgctaa
     5761 gttaaaagtt acaaaaggtg gtccattacc ttttgcttgg gatattttaa gtccacaatt
     5821 catgtatggt tctaaagctt atgttaaaca tccagctgat attccagatt ataaaaaatt
     5881 aagttttcct gaaggtttta aatgggaacg tgtaatgaat tttgaagatg gtggtttagt
     5941 tacagttaca caagacagtt ctttacaaga tggtacttta atctataaag ttaaaatgag
     6001 aggtactaat tttccacctg atggtccagt aatgcaaaag aaaacaatgg gttgggaagc
     6061 aagtacagaa cgtttatatc ctcgtgatgg tgttttaaaa ggtgaaattc accaagcttt
     6121 aaagttaaaa gatggtggtc actacttagt agagtttaaa acaatatata tggctaaaaa
     6181 acctgtacaa ttaccaggtt actattatgt tgatactaaa ttagatatta catcacataa
     6241 tgaagattat acaattgttg aacaatatga acgtagtgaa ggtcgtcatc acttattttt
     6301 atatggtatg gatgaattat ataaataagt actcaagctc gtaacgaagg tcgtgacctt
     6361 gctcgtgaag gtggcgacgt aattcgttca gcttgtaaat ggtctccaga acttgctgct
     6421 gcatgtgaag tttggaaaga aattaaattc gaatttgata ctattgacaa actttaattt
     6481 ttatttttca tgatgtttat gtgaatagca taaacatcgt ttttattttt atggtgttta
     6541 ggttaaatac ctaaacatca ttttacattt ttaaaattaa gttctaaagt tatcttttgt
     6601 ttaaatttgc ctgtctttat aaattacgat gtgccagaaa aataaaatct tagcttttta
     6661 ttatagaatt tatctttatg tattatattt tataagttat aataaaagaa atagtaacat
     6721 actaaagcgg atgtagcgcg tttatcttaa cggaaggcca gtggcagtgg cggtgccact
     6781 gccgaatata aatatggttg agttgcttag tttaccttag cgaaaagaag acttagcagc
     6841 tagccttaac aaacagtttt atattttatg tttgtgttaa ataaaattaa gaaactttag
     6901 ctaaagtttc ccaactcata gaaacgtcat ctaaaattaa agaactgttg taaatttcta
     6961 aaatgattaa taagaatgct gcaaataaaa ggataaatac agccattaaa acagttgtac
     7021 cccagcctgg taatacttta cctgcttctg agttaagtgg acgtaataaa gtacctaatg
     7081 gtgtaactaa accaggttct tggaagtctg aatttacttt tgatggttta gctttagaag
     7141 ttcctgttgc cataattgat taaatgaatt aagcgttatt agcgctattt tatttacttt
     7201 ctgtaaaaaa taaggaaaat attcttcagt gcattccctc tcaggattat aaatactctg
     7261 aggataacgt tctctcgtca aggggttgct tcttgtgagt atagaaacct actagcacaa
     7321 gaaataaatt gcataaaaat gtatttacct aggaccgcag taggcagtcc cttttcccct
     7381 tcagaactgc ctgctttaaa agaatgaaaa aactgccttg tctggtaagt aaaactcttt
     7441 aattactcac taaagacgat ctagaagttc tttgttcatt ttttatttaa tataatattt
     7501 gttatataaa aattaaataa tttttaatta atgtttaact ttgtaaggac agtttcaaag
     7561 tgacatgaat ggctactgca aaaacgaagt aagttattct ttctcagggc aaaattttga
     7621 gtagattaat tttgtttaaa aatgtgggac acagtcgtca agtcttttga actatctaag
     7681 agatatgttg aaaagagaat aattttatta ttaaatgagc tatggaaagt ccagcttttt
     7741 tctttacctt ttttttatgg tttcttctgt taagtgtaac tggctattca gtttatgtta
     7801 gttttggtcc accttcaaaa aaattacgtg atccttttga agaacacgaa gattaaacaa
     7861 gttaaaaagt actattttta caagtgactt cggtgcctct gagaacccta gttatagtga
     7921 tataaaataa gtagctaact actttatatt tttatgaaag tcattttgtc gagcatataa
     7981 acaaaaacaa aattgctata ctaggcagtc acagtgcaac tgtctccgtc tccttaaccg
     8041 agaaagggta aacgtcttcg gtaaagtaac aaactttagt tatgttaact cgcttgcgag
     8101 ttaaccattt tttttcctcc gaaggacaac agttggcagt tgccaaactt tagtggtcta
     8161 atatttatat taggcagttg gcaggcaact gccactgacg tcccgaaggg gaaggggttt
     8221 acttacctcc taacggagta tataaataga ataaaattta tttcctgcgc tagcagattt
     8281 acatactagg attttaatac tccgaaggag gcagtggcgg taccactgcc actggcgtcc
     8341 tccttcccct tcgggcaaat gcattttagt gccacttaag tttacttgcc taggcagttg
     8401 gcaggacgtc agtggcagtg gtaccgcgac tgcctatatt tatatactcc taagtttact
     8461 tgcctaggca gttggcaggc aactgccact gacgtccttc cccttcccct tcgggacgtc
     8521 cccttacggg aatataaata ttagtggata tttatatact gcgatgttta catactccga
     8581 aggaggaagc aggcagttgc ctgccaactg cctaatataa atattgggca agtaaactta
     8641 gaatgtttac atactccgaa ggaggacgtc cccttacggg aatataaata ttagtggcag
     8701 tggtaccgcc actgcctcct tcggagtatt aaaatcctag tatataaata taccgtaagg
     8761 ggacgtcctc cgacggtggc agtggcggta ccactgccac cggcgtccta atatacatat
     8821 tgaagtattt aaacctgtta gcgcagcctc taacgagtca gtaaacttcc cttttggggc
     8881 ttctaggcag cgcataaatt ttctaggacg aacgtccact ggcgtctcgt aaggagcagt
     8941 gacaggccac taatgtcccc ttaatgggta aataaatggc tatcgtctat ccatgaagag
     9001 accatatatt ccagtagcac cgttatgatc ctcaaagggt aacaccattt gtatagtatt
     9061 atggtgaaat gcatcccttt cagggtagat ttatatctta cag
//
