LOCUS       sequence-497976-        9392 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  Bidirectional chloroplast promoter (BDP1) enabling co-expression for
            transformation of Chlamydomonas reinhardtii photosynthetically 
            deficient strain CC4388.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9392)
  TITLE     Engineering bidirectional chloroplast promoters for tunable 
            co-expression of multiple genes in microalgae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pABDP1
FEATURES             Location/Qualifiers
     source          1..9392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18..40)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(234..253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     353..375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(413..431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        499..603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(604..672,673..1464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(822..841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2124..2143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2377..2394
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcccct
     3241 tacgggaata taaatattag tggcagtggt accgccactg cctagtatat aaatatcggc
     3301 agttggcagg atatttatat actccgaagg aacttgttag ccgataggcg aggcaactgc
     3361 cactaaaatt tatttgcctc ctaacggagc attaaaatcc ctaagtttac ttgcccgtaa
     3421 ggggaagggg acgtccacta atatttatat taggcagttg gcaggcaaca ataaatacat
     3481 ttgtcccgta aggggacgtc ctgccaactg cctatggtag ctattaagta tatatatatg
     3541 aaaagtgtgt ataaactaaa ataaaccagg tatggttaac cagatttatt ttagtttaaa
     3601 aaaaaattta tttatataat tcatccatac ctaatgtaat accagcagct gttacaaatt
     3661 ctaataaaac catatgatca cgtttttcat ttggatcttt tgataattta gattgataac
     3721 taagataatg attatcaggt aataaaacag gaccatctcc aattggtgta ttttgttgat
     3781 aatgatcagc taattgaaca ccaccatctt caatattatg acgaatttta aaattagctt
     3841 tgataccatt tttctgttta tcagctgtaa tataaacatt atgtgaatta taattatatt
     3901 ctaatttatg acctaaaata ttaccatctt ctttaaaatc aataccttta agttcaatac
     3961 gattaactaa tgtatctcct tcaaatttaa cttcagcacg tgttttataa ttaccatcat
     4021 ctttaaagaa aattgtacgt tcttgtacat aaccttcagg cattgcactt ttaaagaaat
     4081 catgttgttt catgtgatct ggataacgag caaaacattg taaaccataa cctaatgtag
     4141 taactaaagt tggccatgga actggtaatt tacctgttgt acaaattaat tttaaagtta
     4201 atttaccata agttgcgtca ccttcacctt caccactaac tgaaaatttg tgaccattaa
     4261 cgtcaccatc taattcaact aaaattggaa ctacaccagt gaataattct tcaccttttg
     4321 aaaccatttt atataaataa atgtaacttc ttttgacgat cctaaaataa tctgtccgga
     4381 aatataattt aaaaattgtt gaaaaatttt aaatacattt aaatatatta tatatttaga
     4441 aataaaaaat aatgtaaact agcaaattta tgtacttgtc ttatcagtcg agcatagaaa
     4501 gggcctttag gtatatgtcg gtcgtcttac gcagtttact tactaaaacc atgtatattc
     4561 ctgtcagggg ataagcgagg ccactgttca ctcctctcca atatagtaga ctttattaga
     4621 ggcagtgttt atataccata aacgtcaaaa gtcattttta taactggatc tcaaaatacc
     4681 tataaaccca ttgttcttct cttttagctc taagaacaat caatttataa atatatttat
     4741 tattatgcta taatataaat actatataaa tacatttacc tttttataaa tacatttacc
     4801 ttttttttaa tttgcatgat tttaatgctt atgctatctt ttttatttag tccataaaac
     4861 ctttaaagga ccttttctta tgggatattt atattttcct aacaaagcaa tcggcgtcat
     4921 aaactttagt tgcttacgac gcctgtggac gtccccccct tccccttacg ggcaagtaaa
     4981 cttagggatt ttaatgcaat aaataaattt gtcctcttcg ggcaaatgaa ttttagtatt
     5041 taaatatgac aagggtgaac cattactttt gttaacaagt gatcttacca ctcactattt
     5101 ttgttgaatt ttaaacttat ttaaaattct cgagaaagat tttaaaaata aactttttta
     5161 atcttttatt tattttttct tttttaatgg tatctaaagg tgaagaagta attaaggaat
     5221 tcatgcgttt taaagttcgt atggaaggta gtatgaatgg tcatgaattt gaaattgaag
     5281 gtgagggtga aggtcgtcca tatgaaggta cacaaactgc taagttaaaa gtaactaaag
     5341 gtggtccatt accatttgca tgggatattt tatcaccaca attcatgtat ggttcaaaag
     5401 cttatgtaaa acacccagct gatattcctg actataaaaa attatcattt ccagaaggtt
     5461 ttaaatggga acgtgttatg aactttgaag atggtggttt agtaacagta actcaagatt
     5521 ctagtttaca agatggtaca ttaatatata aagttaaaat gcgtggtact aattttccac
     5581 ctgatggtcc agtaatgcag aaaaagacaa tgggttggga agcttcaact gaacgtttat
     5641 atccacgtga tggtgtacta aaaggtgaaa ttcatcaagc acttaaactt aaagatggtg
     5701 gtcattactt agtagaattc aaaacaatct atatggctaa aaaacctgta caattaccag
     5761 gttattatta tgttgatact aaattagata ttactagtca taatgaagat tatacaattg
     5821 tagaacaata tgaacgttca gaaggtcgtc atcatctgtt tttaggtcat ggtactggat
     5881 ctacaggttc aggtagtagt ggtacagctt cttctgaaga taataatatg gctgttatta
     5941 aggaattcat gcgttttaaa gtaagaatgg aaggtagtat gaatggtcat gaatttgaaa
     6001 ttgaaggtga aggtgaaggt agaccatatg aaggtacaca aactgctaag ttaaaagtta
     6061 caaaaggtgg tccattacct tttgcttggg atattttaag tccacaattc atgtatggtt
     6121 ctaaagctta tgttaaacat ccagctgata ttccagatta taaaaaatta agttttcctg
     6181 aaggttttaa atgggaacgt gtaatgaatt ttgaagatgg tggtttagtt acagttacac
     6241 aagacagttc tttacaagat ggtactttaa tctataaagt taaaatgaga ggtactaatt
     6301 ttccacctga tggtccagta atgcaaaaga aaacaatggg ttgggaagca agtacagaac
     6361 gtttatatcc tcgtgatggt gttttaaaag gtgaaattca ccaagcttta aagttaaaag
     6421 atggtggtca ctacttagta gagtttaaaa caatatatat ggctaaaaaa cctgtacaat
     6481 taccaggtta ctattatgtt gatactaaat tagatattac atcacataat gaagattata
     6541 caattgttga acaatatgaa cgtagtgaag gtcgtcatca cttattttta tatggtatgg
     6601 atgaattata taaataagta ctcaagctcg taacgaaggt cgtgaccttg ctcgtgaagg
     6661 tggcgacgta attcgttcag cttgtaaatg gtctccagaa cttgctgctg catgtgaagt
     6721 ttggaaagaa attaaattcg aatttgatac tattgacaaa ctttaatttt tatttttcat
     6781 gatgtttatg tgaatagcat aaacatcgtt tttattttta tggtgtttag gttaaatacc
     6841 taaacatcat tttacatttt taaaattaag ttctaaagtt atcttttgtt taaatttgcc
     6901 tgtctttata aattacgatg tgccagaaaa ataaaatctt agctttttat tatagaattt
     6961 atctttatgt attatatttt ataagttata ataaaagaaa tagtaacata ctaaagcgga
     7021 tgtagcgcgt ttatcttaac ggaaggccag tggcagtggc ggtgccactg ccgaatataa
     7081 atatggttga gttgcttagt ttaccttagc gaaaagaaga cttagcagct agccttaaca
     7141 aacagtttta tattttatgt ttgtgttaaa taaaattaag aaactttagc taaagtttcc
     7201 caactcatag aaacgtcatc taaaattaaa gaactgttgt aaatttctaa aatgattaat
     7261 aagaatgctg caaataaaag gataaataca gccattaaaa cagttgtacc ccagcctggt
     7321 aatactttac ctgcttctga gttaagtgga cgtaataaag tacctaatgg tgtaactaaa
     7381 ccaggttctt ggaagtctga atttactttt gatggtttag ctttagaagt tcctgttgcc
     7441 ataattgatt aaatgaatta agcgttatta gcgctatttt atttactttc tgtaaaaaat
     7501 aaggaaaata ttcttcagtg cattccctct caggattata aatactctga ggataacgtt
     7561 ctctcgtcaa ggggttgctt cttgtgagta tagaaaccta ctagcacaag aaataaattg
     7621 cataaaaatg tatttaccta ggaccgcggt aggcagtccc ttttcccctt cagaactgcc
     7681 tgctttaaaa gaatgaaaaa actgccttgt ctggtaagta aaactcttta attactcact
     7741 aaagacgatc tagaagttct ttgttcattt tttatttaat ataatatttg ttatataaaa
     7801 attaaataat ttttaattaa tgtttaactt tgtaaggaca gtttcaaagt gacatgaatg
     7861 gctactgcaa aaacgaagta agttattctt tctcagggca aaattttgag tagattaatt
     7921 ttgtttaaaa atgtgggaca cagtcgtcaa gtcttttgaa ctatctaaga gatatgttga
     7981 aaagagaata attttattat taaatgagct atggaaagtc cagctttttt ctttaccttt
     8041 tttttatggt ttcttctgtt aagtgtaact ggctattcag tttatgttag ttttggtcca
     8101 ccttcaaaaa aattacgtga tccttttgaa gaacacgaag attaaacaag ttaaaaagta
     8161 ctatttttac aagtgacttc ggtgcctctg agaaccctag ttatagtgat ataaaataag
     8221 tagctaacta ctttatattt ttatgaaagt cattttgtcg agcatataaa caaaaacaaa
     8281 attgctatac taggcagtca cagtgcaact gtctccgtct ccttaaccga gaaagggtaa
     8341 acgtcttcgg taaagtaaca aactttagtt atgttaactc gcttgcgagt taaccatttt
     8401 ttttcctccg aaggacaaca gttggcagtt gccaaacttt agtggtctaa tatttatatt
     8461 aggcagttgg caggcaactg ccactgacgt cccgaagggg aaggggttta cttacctcct
     8521 aacggagtat ataaatagaa taaaatttat ttcctgcgct agcagattta catactagga
     8581 ttttaatact ccgaaggagg cagtggcggt accactgcca ctggcgtcct ccttcccctt
     8641 cgggcaaatg cattttagtg ccacttaagt ttacttgcct aggcagttgg caggacgtca
     8701 gtggcagtgg taccgcgact gcctatattt atatactcct aagtttactt gcctaggcag
     8761 ttggcaggca actgccactg acgtccttcc ccttcccctt cgggacgtcc ccttacggga
     8821 atataaatat tagtggatat ttatatactg cgatgtttac atactccgaa ggaggaagca
     8881 ggcagttgcc tgccaactgc ctaatataaa tattgggcaa gtaaacttag aatgtttaca
     8941 tactccgaag gaggacgtcc ccttacggga atataaatat tagtggcagt ggtaccgcca
     9001 ctgcctcctt cggagtatta aaatcctagt atataaatat accgtaaggg gacgtcctcc
     9061 gacggtggca gtggcggtac cactgccacc ggcgtcctaa tatacatatt gaagtattta
     9121 aacctgttag cgcagcctct aacgagtcag taaacttccc ttttggggct tctaggcagc
     9181 gcataaattt tctaggacga acgtccactg gcgtctcgta aggagcagtg acaggccact
     9241 aatgtcccct taatgggtaa ataaatggct atcgtctatc catgaagaga ccatatattc
     9301 cagtagcacc gttatgatcc tcaaagggta acaccatttg tatagtatta tggtgaaatg
     9361 catccctttc agggtagatt tatatcttac ag
//
