LOCUS       sequence-497977-       18062 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  Integration plasmid encoding EBOV essential replication factors (NP,
            VP35, VP30, and L protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18062)
  TITLE     bioRxiv preprint: Genome-wide CRISPR knockout screening with viral 
            replicons for identification of host factors involved in viral 
            replication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EBOV 4cis
FEATURES             Location/Qualifiers
     source          1..18062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             603..1631
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             603..1631
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(1643..1660)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1649..1856
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2062..2124
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2175..2193)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2233..3048)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2233..3048)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2956..2975
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3040..3059
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3973..3992
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3973..3991
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4021..4055
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        4595..4898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5347..6363
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6287..6309
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6371..6390
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7972..7983
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8671..8727
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9799..9855
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10771..10827
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(17578..17597)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    17643..17698
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(17697..17716)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gttacagaga tgttacgaac cactagtgca ctgcagtaca gcggtgggct ctatggggta
       61 cctacgtagg tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatagcca
      601 ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
      661 tcgacagcgt gtccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
      721 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
      781 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
      841 acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
      901 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcaaccggtc gcggaggcca
      961 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     1021 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     1081 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     1141 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     1201 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     1261 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     1321 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     1381 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     1441 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     1501 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     1561 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     1621 caaaggaata actcgagcga cgcctcgact gtgccttcta gttgccagcc atctgttgtt
     1681 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1741 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1801 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1861 gtgggctcta tgagtatcga tactagttaa aagttttgtt actttataga agaaattttg
     1921 agtttttgtt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     1981 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     2041 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     2101 cacaatatga ttatctttct agggttaatc tagtatacgc gtatgcggcc gcttaattaa
     2161 tccagctttt gttcccttta gtgagggtta attccgagcg gccgcgtgtt acaaccaatt
     2221 aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     2281 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     2341 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     2401 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     2461 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     2521 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     2581 cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca
     2641 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     2701 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     2761 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     2821 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     2881 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     2941 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     3001 atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     3061 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     3121 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc
     3181 tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3961 gcggccgcca attaatacga ctcactatag ggcgaattgg ggcgcgccat tctagattaa
     4021 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
     4081 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
     4141 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
     4201 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     4261 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     4321 atagatatca actagaatgc tagcatgggc ccagatctcc atgggccctg aattcgcatc
     4381 tagactgaac tggccgataa ttgcagacga ggagcatcgc ccttccccgg ccctcaggta
     4441 agaggaccaa ataccgtagc cgtttccaat ttcagtcctt tagcgccacc tggtgctaac
     4501 tactctatca cgcttttatc caataactac ctttgtaaat ttcctttcaa aagttctggc
     4561 cgggcgcggt ggcaacgcca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     4621 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     4681 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     4741 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     4801 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     4861 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     4921 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     4981 aattattttg tgcagcgatg ggggcggggg gggggggggg gggcgcgcca gggggggggg
     5041 gggggggggg gggggggggg gggggggggg gggggggggg ggggggggcc aaacaagggg
     5101 ggggggcccc gaagggtctt tttttggggg gggggggggg gggggggggc ctaaaaaaag
     5161 ggaaggcgcg gggggggggg gggggggggg cgcgcgcggg gggggggggg gggggggggg
     5221 gggggggggg ggggggggcg gggaggtgcg gcggcagcca atcagagcgg cgcgctccga
     5281 aagtttcctt ttagggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     5341 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     5401 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     5461 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     5521 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     5581 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     5641 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     5701 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     5761 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     5821 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     5881 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     5941 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagt
     6001 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     6061 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     6121 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     6181 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     6241 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     6301 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     6361 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgcc
     6421 actatggata gccgcccaca gaaaatttgg atggcaccat ccctgaccga aagtgatatg
     6481 gactatcata agattttgac tgccggcttg agtgtacaac aagggattgt aagacagaga
     6541 gttatcccgg tctaccaagt gaacaatttg gaagagatct gtcagctgat tatccaggcg
     6601 tttgaagcgg gcgtagattt ccaagaatca gccgattctt ttctccttat gctgtgcctt
     6661 catcacgcat atcaaggtga ttataaactc ttcttggaat ctggcgcggt caaatacttg
     6721 gaagggcatg gtttcagatt cgaggtgaag aaaagggacg gagtcaagag gctggaggag
     6781 cttctgccgg ccgtttctag tggcaaaaac attaaacgca cactcgccgc gatgcctgaa
     6841 gaggagacta cagaagcgaa tgccggccaa tttctgtcat tcgcgtcttt gttcctgcca
     6901 aagctcgtag taggtgaaaa agcatgcctg gaaaaagtac agcgccaaat tcaggtgcat
     6961 gccgaacaag gtcttatcca gtacccaacg gcttggcagt cagttggcca catgatggtg
     7021 atctttagac ttatgcgaac aaactttctg atcaagttcc tgttgattca ccaaggtatg
     7081 catatggtag ccgggcacga cgccaatgat gctgtcataa gcaatagcgt tgcgcaagcg
     7141 cgattttcag ggcttctgat cgtgaaaaca gttctcgatc atattctgca aaagacagaa
     7201 cgcggcgtcc gattgcatcc tctcgcacgg acggctaagg tgaaaaatga ggtcaactct
     7261 ttcaaggccg cactgagctc tctggcaaaa cacggggaat atgccccatt cgcgagactg
     7321 cttaatttgt ctggagtcaa caatctggag catgggcttt ttccgcaact tagtgcgatt
     7381 gccctggggg tagctacagc acacggtagc actttggccg gcgttaacgt cggtgagcaa
     7441 tatcaacaac tgcgagaagc agcgacggag gcagagaaac aactgcaaca gtatgccgag
     7501 tctagggaac tggatcacct cggtctggat gaccaggaaa agaaaattct catgaacttt
     7561 catcaaaaga agaacgagat atcatttcag cagactaatg cgatggtcac tcttcggaaa
     7621 gagcgacttg ctaaactcac agaagcgata acagcagcat ccttgccgaa gacatcaggc
     7681 cactatgacg acgacgacga tattcccttt ccgggtccga ttaacgatga cgataacccg
     7741 ggtcatcaag acgatgatcc cacagactcc caggacacca ccatccccga tgtagttgtg
     7801 gatcctgatg atggctcata cggcgagtac caaagctata gtgagaatgg catgaacgcc
     7861 ccggacgatt tggtattgtt cgacctcgat gaagatgacg aggatactaa gcctgtaccc
     7921 aaccgaagta ccaagggcgg ccagcaaaaa aacagccaaa agggacaaca tattgaaggg
     7981 aggcagacgc aatccagacc gatacaaaat gtcccgggtc cccaccgcac cattcaccat
     8041 gcttccgctc cactcactga caatgataga cgaaatgaac cttcaggttc caccagtcct
     8101 aggatgctca cccccattaa cgaagaggca gatccgctgg atgacgcgga cgacgagact
     8161 agtagccttc cgccgcttga gtctgacgac gaagagcaag atagggatgg cacatctaac
     8221 cggactccta ccgtcgcgcc tccggccccg gtttaccgcg accactccga aaagaaggag
     8281 ctcccacagg acgagcaaca ggaccaagac catacgcaag aggctagaaa tcaagacagt
     8341 gacaacaccc aatctgagca ctcattcgag gagatgtaca ggcatatatt gaggagccaa
     8401 gggccgttcg atgcggtatt gtactatcac atgatgaagg acgaaccggt cgtcttttca
     8461 acctctgacg gaaaggagta tacataccca gacagcctcg aggaggagta cccaccatgg
     8521 cttacggaaa aggaggcgat gaatgaggaa aacaggtttg ttacgctcga tgggcagcaa
     8581 ttctactggc ctgtaatgaa tcataagaat aaatttatgg cgatactgca acaccaccaa
     8641 ggatccggtg ggctgccaag gggttctgga gctactaatt ttagcctttt gaaacaagct
     8701 ggtgatgttg aagagaatcc tggtcccgga acgggcggag gaggtagtat gaccacgcgg
     8761 acaaaaggga gaggccatac cgcagcgacg acacagaatg acaggatgcc aggtcccgaa
     8821 ttgagcgggt ggatttcaga acagcttatg actggaagaa taccggtcag tgacattttt
     8881 tgtgacattg agaataatcc aggtctttgt tatgccagtc aaatgcagca gaccaaacct
     8941 aacccaaaaa caagaaattc ccaaacacag acggacccaa tttgcaacca ttcttttgaa
     9001 gaagtggttc aaacgctcgc atccctggct accgtagttc aacaacaaac tatcgcgagc
     9061 gagagtctcg aacaaaggat aacatcattg gaaaatgggc tgaagcccgt gtatgatatg
     9121 gccaagacga taagtagcct taatcgagtg tgtgctgaaa tggtcgccaa atatgatttg
     9181 cttgtcatga caactgggag agctacagct acggcagcag ccacggaggc gtactgggcg
     9241 gaacacggac agccccctcc cggcccgtcc ctctacgagg aaagtgcgat tcggggaaag
     9301 atcgagagcc gggacgaaac ggtcccacag agcgtccgag aggcctttaa taacctgaac
     9361 tctacgacta gcctgacaga agaaaacttt ggtaagcccg atataagcgc gaaagacctc
     9421 cggaatataa tgtacgacca ccttccggga ttcgggacag cctttcacca actggtccaa
     9481 gtcatctgca aactggggaa ggactccaat tcactggata taatacatgc cgaatttcag
     9541 gcctcattgg cggaaggaga tagtccacaa tgcgcgttga tccagatcac taagcgggtc
     9601 ccaatcttcc aggatgccgc accacctgtg atacatatca ggagtcgggg agacattccg
     9661 cgagcttgtc aaaagagtct ccgaccagtc cccccctctc ctaagataga ccgcggatgg
     9721 gtttgcgttt ttcaattgca agacggcaaa acgttgggat tgaaaatcgg atccggaggg
     9781 ctggatgggg gttccggggc caccaacttc tccctcctta agcaggccgg cgacgtcgag
     9841 gaaaaccccg gcccaggaac gggtggcgga gggagtatgg aggcttctta tgagcgaggg
     9901 cggcctcgcg cagcacgcca gcattccagg gatgggcatg accaccatgt aagagcccgg
     9961 agtagctcaa gagagaatta tcgaggcgaa tacagacaat ccagatccgc gagccaggtt
    10021 cgagtaccga cggtgttcca caagaagcgc gttgaacccc tcactgtccc gccagcccca
    10081 aaagacattt gtcctacgct gaagaagggc tttctttgtg actctagctt ctgtaaaaag
    10141 gatcatcaac tcgagtctct taccgatcgc gagctcctgt tgcttatagc taggaaaaca
    10201 tgcggttccg tggagcaaca acttaatatt acagccccga aagacagcag gctggcaaat
    10261 ccgacggcag acgatttcca acaggaggaa gggccaaaga ttaccttgct cactctcatc
    10321 aaaacggccg agcactgggc aagacaggat attaggacga tagaggactc caaattgcgg
    10381 gccctcttga cattgtgtgc cgtgatgaca cgcaaatttt ccaagagcca actcagcctg
    10441 ctttgtgaaa ctcacctccg aagagaggga ctcgggcaag atcaggctga gccggttctc
    10501 gaggtctacc aaagattgca ttctgataag gggggttcat ttgaagctgc tctttggcag
    10561 caatgggatc gccagagcct tattatgttt attactgcat ttttgaatat cgccttgcag
    10621 ctcccttgcg aatcctctgc ggtcgtcgta agtgggctta ggactctcgt cccacaaagt
    10681 gataacgaag aggcgagcac caatccgggc acctgctcct ggagtgatga aggcacacca
    10741 ggatccggcg ggctggttcg gggatcaggt gcaacaaatt tctcactact caaacaggca
    10801 ggagatgtag aagaaaatcc aggaccggga acgggaggtg gcagtatggc tacacaacat
    10861 acccaatacc cagacgctag gttatcatca ccaattgtat tggaccaatg tgacctagtc
    10921 actagagctt gcgggttata ttcatcatac tcccttaatc cgcaactacg caactgtaaa
    10981 ctcccgaaac atatctaccg tttgaaatac gatgtaactg ttaccaagtt cttgagtgat
    11041 gtaccagtgg cgacattgcc catagatttc atagtcccag ttcttctcaa ggcactgtca
    11101 ggcaatggat tctgtcctgt tgagccgcgg tgccaacagt tcttagatga aatcattaag
    11161 tacacaatgc aagatgctct cttcttgaaa tattatctca aaaatgtggg tgctcaagaa
    11221 gactgtgttg atgaacactt tcaagagaaa atcttatctt caattcaggg caatgaattt
    11281 ttacatcaaa tgtttttctg gtatgatctg gctattttaa ctcgaagggg tagattaaat
    11341 cgaggaaact ctagatcaac atggtttgtt catgatgatt taatagacat cttaggctat
    11401 ggggactatg ttttttggaa gatcccaatt tcaatgttac cactgaacac acaaggaatc
    11461 ccccatgctg ctatggactg gtatcaggca tcagtattca aagaagcggt tcaagggcat
    11521 acacacattg tttctgtttc tactgccgac gtcttgataa tgtgcaaaga tttaattaca
    11581 tgtcgattca acacaactct aatctcaaaa atagcagaga ttgaggatcc agtttgttct
    11641 gattatccca attttaagat tgtgtctatg ctttaccaga gcggagatta cttactctcc
    11701 atattagggt ctgatgggta taaaattatt aagttcctcg aaccattgtg cttggccaaa
    11761 attcaattat gctcaaagta cactgagagg aagggccgat tcttaacaca aatgcattta
    11821 gctgtaaatc acaccctaga agaaattaca gaaatgcgtg cactaaagcc ttcacaggct
    11881 caaaagatcc gtgaattcca tagaacattg ataaggctgg agatgacgcc acaacaactt
    11941 tgtgagctat tttccattca aaaacactgg gggcatcctg tgctacatag tgaaacagca
    12001 atccaaaaag ttaaaaaaca tgctacggtg ctaaaagcat tacgccctat agtgattttc
    12061 gagacatact gtgtttttaa atatagtatt gccaaacatt attttgatag tcaaggatct
    12121 tggtacagtg ttacttcaga taggaatcta acaccgggtc ttaattctta tatcaaaaga
    12181 aatcaattcc ctccgttgcc aatgattaaa gaactactat gggaatttta ccaccttgac
    12241 caccctccac ttttctcaac caaaattatt agtgacttaa gtatttttat aaaagacaga
    12301 gctaccgcag tagaaaggac atgctgggat gcagtattcg agcctaatgt tctaggatat
    12361 aatccacctc acaaatttag tactaaacgt gtaccggaac aatttttaga gcaagaaaac
    12421 ttttctattg agaatgttct ttcctacgca caaaaactcg agtatctact accacaatat
    12481 cggaactttt ctttctcatt gaaagagaaa gagttgaatg taggtagaac cttcggaaaa
    12541 ttgccttatc cgactcgcaa tgttcaaaca ctttgtgaag ctctgttagc tgatggtctt
    12601 gctaaagcat ttcctagcaa tatgatggta gttacggaac gtgagcaaaa agaaagctta
    12661 ttgcatcaag catcatggca ccacacaagt gatgattttg gtgaacatgc cacagttaga
    12721 gggagtagct ttgtaactga tttagagaaa tacaatcttg catttagata tgagtttaca
    12781 gcacctttta tagaatattg caaccgttgc tatggtgtta agaatgtttt taattggatg
    12841 cattatacaa tcccacagtg ttatatgcat gtcagtgatt attataatcc accacataac
    12901 ctcacactgg agaatcgaga caaccccccc gaagggccta gttcatacag gggtcatatg
    12961 ggagggattg aaggactgca acaaaaactc tggacaagta tttcatgtgc tcaaatttct
    13021 ttagttgaaa ttaagactgg ttttaagtta cgctcagctg tgatgggtga caatcagtgc
    13081 attactgttt tatcagtctt ccccttagag actgacgcag acgagcagga acagagcgcc
    13141 gaagacaatg cagcgagggt ggccgccagc ctagcaaaag ttacaagtgc ctgtggaatc
    13201 tttttaaaac ctgatgaaac atttgtacat tcaggtttta tctattttgg aaaaaaacaa
    13261 tatttgaatg gggtccaatt gcctcagtcc cttaaaacgg ctacaagaat ggcaccattg
    13321 tctgatgcaa tttttgatga tcttcaaggg accctggcta gtataggcac tgcttttgag
    13381 cgatccatct ctgagacacg acatatcttt ccttgcagga taaccgcagc tttccatacg
    13441 tttttttcgg tgagaatctt gcaatatcat catctcgggt tcaataaagg ttttgacctt
    13501 ggacagttaa cactcggcaa acctctggat ttcggaacaa tatcattggc actagcggta
    13561 ccgcaggtgc ttggagggtt atccttcttg aatcctgaga aatgtttcta ccggaatcta
    13621 ggagatccag ttacctcagg cttattccag ttaaaaactt atctccgaat gattgagatg
    13681 gatgatttat tcttaccttt aattgcgaag aaccctggga actgcactgc cattgacttt
    13741 gtgctaaatc ctagcggatt aaatgtccct gggtcgcaag acttaacttc atttctgcgc
    13801 cagattgtac gcaggaccat caccctaagt gcgaaaaaca aacttattaa taccttattt
    13861 catgcgtcag ctgacttcga agacgaaatg gtttgtaaat ggctattatc atcaactcct
    13921 gttatgagtc gttttgcggc cgatatcttt tcacgcacgc cgagcgggaa gcgattgcaa
    13981 attctaggat acctggaagg aacacgcaca ttattagcct ctaagatcat caacaataat
    14041 acagagacac cggttttgga cagactgagg aaaataacat tgcaaaggtg gagcctatgg
    14101 tttagttatc ttgatcattg tgataatatc ctggcggagg ctttaaccca aataacttgc
    14161 acagttgatt tagcacagat tctgagggaa tattcatggg ctcatatttt agagggaaga
    14221 cctcttattg gagccacact cccatgtatg attgagcaat tcaaagtgtt ttggctgaaa
    14281 ccctacgaac aatgtccgca gtgttcaaat gcaaagcaac caggtgggaa accattcgtg
    14341 tcagtggcag tcaagaaaca tattgttagt gcatggccga acgcatcccg aataagctgg
    14401 actatcgggg atggaatccc atacattgga tcaaggacag aagataagat aggacaacct
    14461 gctattaaac caaaatgtcc ttccgcagcc ttaagagagg ccattgaatt ggcgtcccgt
    14521 ttaacatggg taactcaagg cagttcgaac agtgacttgc taataaaacc atttttggaa
    14581 gcacgagtaa atttaagtgt tcaagaaata cttcaaatga ccccttcaca ttactcagga
    14641 aatattgttc acaggtacaa cgatcaatac agtcctcatt ctttcatggc caatcgtatg
    14701 agtaattcag caacgcgatt gattgtttct acaaacactt taggtgagtt ttcaggaggt
    14761 ggccagtctg cacgcgacag caatattatt ttccagaatg ttataaatta tgcagttgca
    14821 ctgttcgata ttaaatttag aaacactgag gctacagata tccaatataa tcgtgctcac
    14881 cttcatctaa ctaagtgttg cacccgggaa gtaccagctc agtatttaac atacacatct
    14941 acattggatt tagatttaac aagataccga gaaaacgaat tgatttatga cagtaatcct
    15001 ctaaaaggag gactcaattg caatatctca ttcgataatc catttttcca aggtaaacgg
    15061 ctgaacatta tagaagatga tcttattcga ctgcctcact tatctggatg ggagctagcc
    15121 aagaccatca tgcaatcaat tatttcagat agcaacaatt catctacaga cccaattagc
    15181 agtggagaaa caagatcatt cactacccat ttcttaactt atcccaagat aggacttctg
    15241 tacagttttg gggcctttgt aagttattat cttggcaata caattcttcg gactaagaaa
    15301 ttaacacttg acaatttttt atattactta actactcaaa ttcataatct accacatcgc
    15361 tcattgcgaa tacttaagcc aacattcaaa catgcaagcg ttatgtcacg gttaatgagt
    15421 attgatcctc atttttctat ttacataggc ggtgctgcag gtgacagagg actctcagat
    15481 gcggccaggt tatttttgag aacgtccatt tcatcttttc ttacatttgt aaaagaatgg
    15541 ataattaatc gcggaacaat tgtcccttta tggatagtat atccgctaga gggtcaaaac
    15601 ccaacacctg tgaataattt tctctatcag atcgtagaac tgctggtgca tgattcatca
    15661 agacaacagg cttttaaaac taccataagt gatcatgtac atcctcacga caatcttgtt
    15721 tacacatgta agagtacagc cagcaatttc ttccatgcat cattggcgta ctggaggagc
    15781 agacacagaa acagcaaccg aaaatacttg gcaagagact cttcaactgg atcaagcaca
    15841 aacaacagtg atggtcatat tgagagaagt caagaacaaa ccaccagaga tccacatgat
    15901 ggcactgaac ggaatctagt cctacaaatg agccatgaaa taaaaagaac gacaattcca
    15961 caagaaaaca cgcaccaggg tccgtcgttc cagtcctttc taagtgactc tgcttgtggt
    16021 acagcaaatc caaaactaaa tttcgatcga tcgagacaca atgtgaaatt tcaggatcat
    16081 aactcggcat ccaagaggga aggtcatcaa ataatctcac accgtctagt cctacctttc
    16141 tttacattat ctcaagggac acgccaatta acgtcatcca atgagtcaca aacccaagac
    16201 gagatatcaa agtacttacg gcaattgaga tccgtcattg ataccacagt ttattgtaga
    16261 tttaccggta tagtctcgtc catgcattac aaacttgatg aggtcctttg ggaaatagag
    16321 agtttcaagt cggctgtgac gctagcagag ggagaaggtg ctggtgcctt actattgatt
    16381 cagaaatacc aagttaagac cttatttttc aacacgctag ctactgagtc cagtatagag
    16441 tcagaaatag tatcaggaat gactactcct aggatgcttc tacctgttat gtcaaaattc
    16501 cataatgacc aaattgagat tattcttaac aactcagcaa gccaaataac agacataaca
    16561 aatcctactt ggtttaaaga ccaaagagca aggctaccta agcaagtcga ggttataacc
    16621 atggatgcag agacaacaga gaatataaac agatcgaaat tgtacgaagc tgtatataaa
    16681 ttgatcttac accatattga tcctagcgta ttgaaagcag tggtccttaa agtctttcta
    16741 agtgatactg agggtatgtt atggctaaat gataatttag ccccgttttt tgccactggt
    16801 tatttaatta agccaataac gtcaagtgct agatctagtg agtggtatct ttgtctgacg
    16861 aacttcttat caactacacg taagatgcca caccaaaacc atctcagttg taaacaggta
    16921 atacttacgg cattgcaact gcaaattcaa cgaagcccat actggctaag tcatttaact
    16981 cagtatgctg actgtgagtt acatttaagt tatatccgcc ttggttttcc atcattagag
    17041 aaagtactat accacaggta taacctcgtc gattcaaaaa gaggtccact agtctctatc
    17101 actcagcact tagcacatct tagagcagag attcgagaat taactaatga ttataatcaa
    17161 cagcgacaaa gtcggactca aacatatcac tttattcgta ctgcaaaagg acgaatcaca
    17221 aaactagtca atgattattt aaaattcttt cttattgtgc aagcattaaa acataatggg
    17281 acatggcaag ctgagtttaa gaaattacca gagttgatta gtgtgtgcaa taggttctac
    17341 catattagag attgcaattg tgaagaacgt ttcttagttc aaaccttata tttacataga
    17401 atgcaggatt ctgaagttaa gcttatcgaa aggctgacag ggcttctgag tttatttccg
    17461 gatggactct acaggtttga tggatcctaa ctcgagattc actcctcagg tgcaggctgc
    17521 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
    17581 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
    17641 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
    17701 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
    17761 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
    17821 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
    17881 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
    17941 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
    18001 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatgctgag
    18061 ca
//
