LOCUS       sequence-497979-        9225 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  Bidirectional chloroplast promoter (BDP2) enabling co-expression for
            transformation of Chlamydomonas reinhardtii photosynthetically 
            deficient strain CC4388.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  TITLE     Engineering bidirectional chloroplast promoters for tunable 
            co-expression of multiple genes in microalgae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pABDP2
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9..25)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18..40)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(234..253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     353..375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(413..431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        499..603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(604..672,673..1464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(822..841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2124..2143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2377..2394
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(3611..3634)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcccct
     3241 tacgggaata taaatattag tggcagtggt accgccactg cctagtatat aaatatcggc
     3301 agttggcagg atatttatat actccgaagg aacttgttag ccgataggcg aggcaactgc
     3361 cactaaaatt tatttgcctc ctaacggagc attaaaatcc ctaagtttac ttgcccgtaa
     3421 ggggaagggg acgtccacta atatttatat taggcagttg gcaggcaaca ataaatacat
     3481 ttgtcccgta aggggacgtc ctgccaactg cctatggtag ctattaagta tatatatatg
     3541 aaaagtgtgt ataaactaaa ataaaccagg tatggttaac cagatttatt ttagtttaaa
     3601 aaaaaattta cttctcaaac tgaggatgcg accagaactc gatgtatttt agtttaaaaa
     3661 aaaatttatt tatataattc atccatacct aatgtaatac cagcagctgt tacaaattct
     3721 aataaaacca tatgatcacg tttttcattt ggatcttttg ataatttaga ttgataacta
     3781 agataatgat tatcaggtaa taaaacagga ccatctccaa ttggtgtatt ttgttgataa
     3841 tgatcagcta attgaacacc accatcttca atattatgac gaattttaaa attagctttg
     3901 ataccatttt tctgtttatc agctgtaata taaacattat gtgaattata attatattct
     3961 aatttatgac ctaaaatatt accatcttct ttaaaatcaa tacctttaag ttcaatacga
     4021 ttaactaatg tatctccttc aaatttaact tcagcacgtg ttttataatt accatcatct
     4081 ttaaagaaaa ttgtacgttc ttgtacataa ccttcaggca ttgcactttt aaagaaatca
     4141 tgttgtttca tgtgatctgg ataacgagca aaacattgta aaccataacc taatgtagta
     4201 actaaagttg gccatggaac tggtaattta cctgttgtac aaattaattt taaagttaat
     4261 ttaccataag ttgcgtcacc ttcaccttca ccactaactg aaaatttgtg accattaacg
     4321 tcaccatcta attcaactaa aattggaact acaccagtga ataattcttc accttttgaa
     4381 accattataa aatttttata agattgtgac atgaccatta ggctttctta agactaaaaa
     4441 aatgtgtagc ttaattattt aaagtttaaa ttaaagttat aatatatatt atatataaaa
     4501 taaaaaaaac gttagtaatt caaaagtttt aatattatac aattgaacta ttatgtatta
     4561 aatataagaa tgtcacctct taccatattt ctatactcca aagtaacttt ttacataaat
     4621 gtcccctctg gggctgcctc cttccccttc cccttcgggt atataaatat agggcaagta
     4681 aacttagcat aaactttagt tgcccgaagg ggtttacata ctccgaagga ggacaaattt
     4741 atttattgtg gtacaataaa taaattgtat gtaaacccct ttcgggtaac taaagtttat
     4801 cacggcaata agtttctgct tacgcagtat tatatctgac gcagtattat ataagaagtt
     4861 ggcaggataa aaatgtgtaa gtatggcaat cttttaaaat agtgttcaat tcatttaagg
     4921 cagataaaaa gaaaaaagtc cacaggattt aattttgaat agttctctat caaaaaaagg
     4981 tttgccgaac aatgttttta ttcctggagt ttgattttaa tggtatctaa aggtgaagaa
     5041 gtaattaagg aattcatgcg ttttaaagtt cgtatggaag gtagtatgaa tggtcatgaa
     5101 tttgaaattg aaggtgaggg tgaaggtcgt ccatatgaag gtacacaaac tgctaagtta
     5161 aaagtaacta aaggtggtcc attaccattt gcatgggata ttttatcacc acaattcatg
     5221 tatggttcaa aagcttatgt aaaacaccca gctgatattc ctgactataa aaaattatca
     5281 tttccagaag gttttaaatg ggaacgtgtt atgaactttg aagatggtgg tttagtaaca
     5341 gtaactcaag attctagttt acaagatggt acattaatat ataaagttaa aatgcgtggt
     5401 actaattttc cacctgatgg tccagtaatg cagaaaaaga caatgggttg ggaagcttca
     5461 actgaacgtt tatatccacg tgatggtgta ctaaaaggtg aaattcatca agcacttaaa
     5521 cttaaagatg gtggtcatta cttagtagaa ttcaaaacaa tctatatggc taaaaaacct
     5581 gtacaattac caggttatta ttatgttgat actaaattag atattactag tcataatgaa
     5641 gattatacaa ttgtagaaca atatgaacgt tcagaaggtc gtcatcatct gtttttaggt
     5701 catggtactg gatctacagg ttcaggtagt agtggtacag cttcttctga agataataat
     5761 atggctgtta ttaaggaatt catgcgtttt aaagtaagaa tggaaggtag tatgaatggt
     5821 catgaatttg aaattgaagg tgaaggtgaa ggtagaccat atgaaggtac acaaactgct
     5881 aagttaaaag ttacaaaagg tggtccatta ccttttgctt gggatatttt aagtccacaa
     5941 ttcatgtatg gttctaaagc ttatgttaaa catccagctg atattccaga ttataaaaaa
     6001 ttaagttttc ctgaaggttt taaatgggaa cgtgtaatga attttgaaga tggtggttta
     6061 gttacagtta cacaagacag ttctttacaa gatggtactt taatctataa agttaaaatg
     6121 agaggtacta attttccacc tgatggtcca gtaatgcaaa agaaaacaat gggttgggaa
     6181 gcaagtacag aacgtttata tcctcgtgat ggtgttttaa aaggtgaaat tcaccaagct
     6241 ttaaagttaa aaaatggtgg tcactactta gtagagttta aaacaatata tatggctaaa
     6301 aaacctgtac aattaccagg ttactattat gttgatacta aattagatat tacatcacat
     6361 aatgaagatt atacaattgt tgaacaatat gaacgtagtg aaggtcgtca tcacttattt
     6421 ttatatggta tggatgaatt atataaataa gtactcaagc tcgtaacgaa ggtcgtgacc
     6481 ttgctcgtga aggtggcgac gtaattcgtt cagcttgtaa atggtctcca gaacttgctg
     6541 ctgcatgtga agtttggaaa gaaattaaat tcgaatttga tactattgac aaactttaat
     6601 ttttattttt catgatgttt atgtgaatag cataaacatc gtttttattt ttatggtgtt
     6661 taggttaaat acctaaacat cattttacat ttttaaaatt aagttctaaa gttatctttt
     6721 gtttaaattt gcctgtcttt ataaattacg atgtgccaga aaaataaaat cttagctttt
     6781 tattatagaa tttatcttta tgtattatat tttataagtt ataataaaag aaatagtaac
     6841 atactaaagc ggatgtagcg cgtttatctt aacggaaggc cagtggcagt ggcggtgcca
     6901 ctgccgaata taaatatggt tgagttgctt agtttacctt agcgaaaaga agacttagca
     6961 gctagcctta acaaacagtt ttatatttta tgtttgtgtt aaataaaatt aagaaacttt
     7021 agctaaagtt tcccaactca tagaaacgtc atctaaaatt aaagaactgt tgtaaatttc
     7081 taaaatgatt aataagaatg ctgcaaataa aaggataaat acagccatta aaacagttgt
     7141 accccagcct ggtaatactt tacctgcttc tgagttaagt ggacgtaata aagtacctaa
     7201 tggtgtaact aaaccaggtt cttggaagtc tgaatttact tttgatggtt tagctttaga
     7261 agttcctgtt gccataattg attaaatgaa ttaagcgtta ttagcgctat tttatttact
     7321 ttctgtaaaa aataaggaaa atattcttca gtgcattccc tctcaggatt ataaatactc
     7381 tgaggataac gttctctcgt caaggggttg cttcttgtga gtatagaaac ctactagcac
     7441 aagaaataaa ttgcataaaa atgtatttac ctaggaccgc agtaggcagt cccttttccc
     7501 cttcagaact gcctgcttta aaagaatgaa aaaactgcct tgtctggtaa gtaaaactct
     7561 ttaattactc actaaagacg atctagaagt tctttgttca ttttttattt aatataatat
     7621 ttgttatata aaaattaaat aatttttaat taatgtttaa ctttgtaagg acagtttcaa
     7681 agtgacatga atggctactg caaaaacgaa gtaagttatt ctttctcagg gcaaaatttt
     7741 gagtagatta attttgttta aaaatgtggg acacagtcgt caagtctttt gaactatcta
     7801 agagatatgt tgaaaagaga ataattttat tattaaatga gctatggaaa gtccagcttt
     7861 tttctttacc ttttttttat ggtttcttct gttaagtgta actggctatt cagtttatgt
     7921 tagttttggt ccaccttcaa aaaaattacg tgatcctttt gaagaacacg aagattaaac
     7981 aagttaaaaa gtactatttt tacaagtgac ttcggtgcct ctgagaaccc tagttatagt
     8041 gatataaaat aagtagctaa ctactttata tttttatgaa agtcattttg tcgagcatat
     8101 aaacaaaaac aaaattgcta tactaggcag tcacagtgca actgtctccg tctccttaac
     8161 cgagaaaggg taaacgtctt cggtaaagta acaaacttta gttatgttaa ctcgcttgcg
     8221 agttaaccat tttttttcct ccgaaggaca acagttggca gttgccaaac tttagtggtc
     8281 taatatttat attaggcagt tggcaggcaa ctgccactga cgtcccgaag gggaaggggt
     8341 ttacttacct cctaacggag tatataaata gaataaaatt tatttcctgc gctagcagat
     8401 ttacatacta ggattttaat actccgaagg aggcagtggc ggtaccactg ccactggcgt
     8461 cctccttccc cttcgggcaa atgcatttta gtgccactta agtttacttg cctaggcagt
     8521 tggcaggacg tcagtggcag tggtaccgcg actgcctata tttatatact cctaagttta
     8581 cttgcctagg cagttggcag gcaactgcca ctgacgtcct tccccttccc cttcgggacg
     8641 tccccttacg ggaatataaa tattagtgga tatttatata ctgcgatgtt tacatactcc
     8701 gaaggaggaa gcaggcagtt gcctgccaac tgcctaatat aaatattggg caagtaaact
     8761 tagaatgttt acatactccg aaggaggacg tccccttacg ggaatataaa tattagtggc
     8821 agtggtaccg ccactgcctc cttcggagta ttaaaatcct agtatataaa tataccgtaa
     8881 ggggacgtcc tccgacggtg gcagtggcgg taccactgcc accggcgtcc taatatacat
     8941 attgaagtat ttaaacctgt tagcgcagcc tctaacgagt cagtaaactt cccttttggg
     9001 gcttctaggc agcgcataaa ttttctagga cgaacgtcca ctggcgtctc gtaaggagca
     9061 gtgacaggcc actaatgtcc ccttaatggg taaataaatg gctatcgtct atccatgaag
     9121 agaccatata ttccagtagc accgttatga tcctcaaagg gtaacaccat ttgtatagta
     9181 ttatggtgaa atgcatccct ttcagggtag atttatatct tacag
//
