LOCUS       sequence-49822-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Yamamoto Y, Takeshita H, Sawa H
  TITLE     Multiple Wnts redundantly control polarity orientation in 
            Caenorhabditis elegans epithelial stem cells.
  JOURNAL   PLoS Genet. 2011 Oct;7(10):e1002308. Epub 2011 Oct 13.
  PUBMED    22022276
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2011-10"; volume: "7(10)"; pages: "e1002308. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pPD95.75-wVenus
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    345..366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(381..386,387..404,405..411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     424..446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     443..459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     443..459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      301 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      361 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      421 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa
      481 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
      541 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc
      601 ctgcaggtcg actctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga
      661 atgatactaa cataacatag aacattttca ggaggaccct tggagggtac cggtagaaaa
      721 aatgtccaaa ggtgaggagc ttttcacag
//
