LOCUS       sequence-498275-        8530 bp    DNA     circular SYN 26-JAN-2026
DEFINITION  Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10, and thereby produces lycopene in 
            Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Cunningham FX Jr, Lee H, Gantt E
  TITLE     Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon 
            merolae.
  JOURNAL   Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.
  PUBMED    17085635
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2007-03"; volume: "6(3)"; pages: "533-45. Epub 2006 
            Nov 3"
COMMENT     Sequence Label: pAC-LYCipi
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..545
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     446..465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     662..679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1071..1173
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1174..1833
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1240..1259)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2042..2061
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(8391..8396,8397..8413,8414..8419))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gcagggggaa acgcctggta tctttatagt cctgtcgggt
      481 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
      541 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
      601 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
      661 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
      721 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
      781 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac
      841 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag
      901 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
      961 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     1021 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     1081 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1141 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1381 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1681 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1801 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1861 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1921 aaagcaaatt cgaccccaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     1981 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     2041 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     2101 cgatgcgtcc ggcgtagagg atctaccacc tgcgtgcgct gggtgttgat gtgcgcctga
     2161 cccgctccga cgtgacaaag ggccacccgg agtactatca ggactacgcc cgccgcctgg
     2221 cagaggagac gccgggtgct tactatattg atcagttctc taacccggcc aacccgctgg
     2281 cgcacgccac caccaccgca ccggagctgt tcgaccagct ggcaggcagg gttgatgccg
     2341 tggtagtagg cgttggctcc ggcggcacgc tgggtggctt gcaggcatgg tttgccaaac
     2401 actctccgca taccgaattc gtgctggccg atcccgttgg ctcgattctg gcggatcagg
     2461 ttgagaccgg acagcacggc gaggcgggat cccgcgccgg ggctacctga agcggctcta
     2521 cgtcgcccac cgcctgcacc acgcggtgcg cggccgggag ggctgcgtct ccttcggttt
     2581 tatttacgcc cgcaagcctg ccgacctaca ggcgatcctg cgtgaacgtc atggccgccc
     2641 gcctaaacgg gacgctgcca aagaccggcc ggacgcggcg tcaccctcgt cgtcttcgcc
     2701 cgaataacct gccccggtgc cgccatcagc atggcaattt tttcaccttt gctggtgtgc
     2761 tggcggcgat cccaggcgct gcctcccgcc gcttttacct taataccgat ctcccggtag
     2821 acgctgcggg cggtggcgat cgcccaggcg cagcgcggcg gcagatcgtg tagcccggcc
     2881 tgggaggaga tgtagtacgg ctctgcggca tcaataagcc gctccgccac ccgcgccagc
     2941 gcggcccgat tctcccgcgc ggcatagttc tccggggtca gcccggcatc ctgcagccac
     3001 tcggcgggca gatagcagcg gtcaatagcc gcatcgtcaa taatatcccg ggcgatattc
     3061 gtcagctgga aggccagccc cagatcgcag gcgcgatcca gcacccgctc atcccgcacg
     3121 cccatcaccc tggccatcat cagacccacc acgcccgcca cgtgatagca gtagcgcagc
     3181 gtatcctcaa aggtgacata gcgggtctga gccacgtcca tcgcaaagcc gtcgaggtga
     3241 tcgagcgcca tgcggggcgt aataccgtgg gtcagcgcca cctcctgaaa ggcagcgaag
     3301 gccggatcct gcatctcggc cccttcaaac gccgccaggg tcagcgtgcg cagccgggcc
     3361 aggcgctggg tggcctcctc ctccgccgcg gcctcgctgg cgaagccgtg ggtctggtcg
     3421 tcaatgacgt catcgcagtg gcggcaccag gtgtagagca tcagcacgct acggcgggtg
     3481 gccgggtcga acagcttcgc agcggtggca aaacttttcg agccgttggc catggtctgc
     3541 gtggcgtggt caagcagcgg cggttggctc attgcagatc ctcaatcatc aggctggcgg
     3601 tggctttcgc cgaggccact acgccaggaa tgcccgcccc agggtgagta cctgcgccca
     3661 ccaggtagag gttggcaatg tcgctgtcgc ggttgtgcgg gcggaaccag gcgctttggg
     3721 tcagcagcgg ctcgatggag aaggccgatc ccagatgcgc atccagcgtg tcgtggaagt
     3781 ctgccggggt aaagatccgc tgggtcacca gctggctacg caggccgggc atatagcgct
     3841 cttcaaggta gtcaaagatg cggtcgcgca gcttcggccc ctcctgcgcc cagtccagcg
     3901 gcgcgttgcc aagatgcggc accggggcca gcacgtagaa gctggcgcag ccgggaggcg
     3961 cgagcgaggg atcggtcacg cagggcgagt gcaggtagag cgagaagtca tccgccagcg
     4021 cgctgccggt aaagatctcg tcgatcagct cccggtagcg gggaccaaaa cagatggtat
     4081 ggtgcgccag ctgggaatga ggctggttca ggccgaagta gagcacaaac agcgagttgc
     4141 tcatgctctt gcgctccagc gctgccgccc gcttctgccc caccggatgg tggccgagca
     4201 gctttttata ggtgttcacc acgtcagcgt tcgaggctac ggcgtcggtg tcaaagatcc
     4261 gaccatccgc cagccggacc tggcttacgc ggttatcggc caccaccagc tcttcgaccc
     4321 gggcgttgag ttcgatctcc ccgcccagat cggtaaacag cttcaccatg ccgttcacca
     4381 gcgccccggt gccgccctca gggaaccaga ccccccactc ccgctcaagg gcgtggatca
     4441 gggtgtagat ggacgaggtg gtgaaggggt tgccgcctac cagcagggag tggaacgaga
     4501 aggcctgccg cagatgctca tcctcaataa agcgcgaaac cgactggtag acgctctgcc
     4561 acgcctggag cttaagcagc tgcggcccgg cgcgcagcat gtcgcgaaaa gagaggaacg
     4621 gcacgctgcc gaggcgcaaa tatccctcct ggaataccgc ctgggagtaa gccagaaagc
     4681 gccggtagcc ctcgacgtcg cgggggttga actgggtaat ctgcgcctca agctcggcgc
     4741 tgtcgttagc atagtcgagg gtcttcccgg actcccagca gagtcggtag aagggtttta
     4801 ccggcagcag cctgacgtaa tcctccatgc gcctgccggc cagggtgaac agcgcctcaa
     4861 gcgcggtagg atcggtgatc accgtcggcc cggcgtcaaa ggtaaagccc tggtcatgcc
     4921 agacgtaggc ccgaccgccg ggcttgtccc gctgctccag cagtacggtt gggatccctg
     4981 ccgcctgcag gcgaatcgcc agcgccaggc caccaaagcc tgcgccaatc acaacggttt
     5041 ttttcatcct ttatctcgtc tgtcaggaaa atggttcagc gccgcccgcc aggcttcgcc
     5101 cagcggaacc ggtggcttgc ccgtcaaaat gcgggcctta tcaaagagag agagccgacc
     5161 ggcgtaaaag cgctctacgg tgggctccgg cagcccataa aagcgctgca tcacccgcca
     5221 gcggttctcc tcgcgcccgg ccaggaaaag catccggttc agcaggcgga agaatccctg
     5281 cctgcgccag tggcgttcgg caaactgccg ggtgagctga tagagcggaa cgctgcccag
     5341 ccgcgggctg tcggcaatcg cgtcggcaag ggccaccgcc agcggcagcg aatagccagt
     5401 ggtagggtga aatagcccag cccgcattcc cgaggcaata aggggcatct gcggccaggc
     5461 ctcggcaatg tcaaccagcg cctcagcggt ggggatcccc cagtcggcaa agacgttggc
     5521 cacgctgcgc tgctgctcgg tggccgcccg ctcgccttca acggcggccc agctggtgcc
     5581 gcccgcgccc gcgacgtcaa tcacggtaac gccggcatcg atcagctgcc cggccacggt
     5641 tcgggagata ccggctccca cctctttcac caccagcgga acgggcagct cgcggaccag
     5701 agtttcaata gccgccagcc gtccgcgcca gtcgcgatcg ccgccgggct gtagcgcctc
     5761 ctgcagcggg tttaggtgca caatcagcgc atccgcctcg atcatctcca cggcccgtcg
     5821 ggcgtaatca ataccttttc tgccggtcag ctgcgccgcg ccgaggttcg ccagcagcgg
     5881 cacgtccgga gccagctgcc gcagggtttt atccagccct aagcccgcgt cgctctcaat
     5941 ggcgacgcgc tgggagccca cccccatcgc aatttttagc acctgcgccg cctcggcgag
     6001 gtggcggttg atatggcgcg agcgctcaac gccgccggtc atggagctga tcagcagcgg
     6061 agcctgtagc tgccgattca ggaaggtggt ttccagcgtg atgtcgctaa aattcagctc
     6121 tggcagggcg cagtgggtaa agcgccagcg ctcaaaacct gcgctagcct gagttacggc
     6181 gcgacggggg tcgagaacga tgtcggcaaa gacgttggcc acgctgcgct gctgctcggt
     6241 ggccgcccgc tcgccttcaa cggcggccca gctggtgccg cccgcgcccg cgacgtcaat
     6301 cacggtaacg ccggcatcga tcagctgccc ggccacggtt cgggagatac cggctcccac
     6361 ctctttcacc accagcggaa cgggcagctc gcggaccaga gtttcaatag ccgccagccg
     6421 tccgcgccag tcgcgatcgc cgccgggctg tagcgcctcc tgcagcgggt ttaggtgcac
     6481 aatcagcgca tccgcctcga tcatctccac ggcccgtcgg gcgtaatcaa taccttttct
     6541 gccggtcagc tgcgccgcgc cgaggttcgc cagcagcggc acgtccggag ccagctgccg
     6601 cagggtttta tccagcccta agcccgcgtc gctctcaatg gcgacgcgct gggagcccac
     6661 ccccatcgca atttttagca cctgcgccgc ctcggcgagg tggcggttga tatggcgcga
     6721 gcgctcaacg ccgccggtca tggagctgat cagcagcgga gcctgtagct gccgattcag
     6781 gaaggtggtt tccagcgtga tgtcgctaaa attcagctct ggcagggcgc agtgggtaaa
     6841 gcgccagcgc tcaaaacctg cgctagcctg agttacggcg cgacgggggt cgagaacgat
     6901 atccagatga tcgttcttac gctgaacaag gcgctcgtcc ttcattcatc actactcctg
     6961 taatgtggtt ccatcgttat gtactgcttg aacccaaaag ggcggtatca ggcgattttc
     7021 atgaccggtg accagtcggc aaggtgatgg ccaaaccaca gatgcataaa ctgtcggatg
     7081 gcgccgccct gcggacaggc aaaagtgagg tgtttgtcgg cggaatcaat atgctcgcgc
     7141 agcttttgcc gggccgcgtc tgcgcccagc cggttgacca gcgtcgattt tcccgcgtcc
     7201 ttattgcgat ctttaccggt ttccgggtga tcgtcacgca gatcgtccag cagttgaaac
     7261 gcctggccga agtcgagggc gaaggcgtgc agcgtctctc gcgtgctcgg cgacgaggcg
     7321 gaagcaatgg cgacgatctg cagcatcgcg ctgaacagaa tgccggtctt gaggtggttg
     7381 gtgctgagga tagcgtcagg ggtacggtcg agggcggcat cgttaagatc gcgaaactgc
     7441 cccagtacca ggccctgcac gcccacggcg gtagagagct cgttgaccgc ctgggcacgc
     7501 ctctcccccg gcagatcgcc ggtggcggcg atcagaccaa aggctttaga gagcagccca
     7561 acggaggcaa ggatcgccac gctctcacca aattttttgt gggtagtggg ctgaccgcgg
     7621 cgcagctcgg cgttgtccat gcagggcatg tcgtcgagca tcagcgacgc ggtatgggtc
     7681 agttcaacgg cgcaggcgag atcgagcagc gtaggcatac tgccctggta gcggaggtcg
     7741 cgggcggcca gcagcatcag cagcggacgg atccgtttac cgggtgccat gacgccttca
     7801 cgcatcgcaa ggctgacgat atcctggctg tcggtttcag gtaacaggcc agccaggtga
     7861 tcgtcaatgg attgtctcat tacttctatt tcgcgatgag gcgaaacgcc cgctttactg
     7921 ccactcacca ttgcaaggtt acccgctgaa ttgtctttta gtatcagtta accgtagacc
     7981 gcgcggatag ctctcgccag gttctgaagc agttttacat tgtgcgaagc atgagacgct
     8041 gtgcctttag atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
     8101 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
     8161 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
     8221 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
     8281 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
     8341 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
     8401 atcgatgata agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag
     8461 gtgctacatt tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa
     8521 gatgatcttc
//
