LOCUS       sequence-49852-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Johnson KM, Kines RC, Roberts JN, Lowy DR, Schiller JT, Day PM
  TITLE     Role of heparan sulfate in attachment to and infection of the murine
            female genital tract by human papillomavirus.
  JOURNAL   J Virol. 2009 Mar;83(5):2067-74. Epub 2008 Dec 10.
  PUBMED    19073722
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2009-03"; volume: "83(5)"; pages: "2067-74. Epub 2008 Dec 10"
COMMENT     Sequence Label: pCLucf
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          170..302
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     285..304
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        347..365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      415..424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      121 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      181 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      241 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      301 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
      361 ataggctagc ctcgagaatt ctctagagct tggcaatccg gtactgttgg taaagccacc
      421 atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg
      481 accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc
      541 gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc
      601 gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg
      661 tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg
      721 gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc
      781 agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa
      841 aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc
      901 ttccaaagca tgtacacctt cgtgacttcc catttgccca c
//
