LOCUS       sequence-49873-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Hsu PP, Kang SA, Rameseder J, Zhang Y, Ottina KA, Lim D, Peterson 
            TR, Choi Y, Gray NS, Yaffe MB, Marto JA, Sabatini DM
  TITLE     The mTOR-regulated phosphoproteome reveals a mechanism of 
            mTORC1-mediated inhibition of growth factor signaling.
  JOURNAL   Science. 2011 Jun 10;332(6035):1317-22.
  PUBMED    21659604
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-06-10"; volume: "332(6035)"; pages: "1317-22"
COMMENT     Sequence Label: pRK5 Flag Grb10 9A long
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..84
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aggtgtcact ccaggtccaa ctgcacctcg gttctatcga ttgaattcac catggaaatg
       61 gactacaagg atgacgatga caagggtggt ggtggtggtg cgtcgacgat ggctttagcc
      121 ggctgcccag attccttttt gcaccatccg tactaccagg acaaggtgga gcagacacct
      181 cgcagtcaac aagacccggc aggaccagga ctccccgcac agtctgaccg acttgcgaat
      241 caccaggagg atgatgtgga cctggaagcc ctggtgaacg atatgaatgc atccctggag
      301 agcctgtact cggcctgcag catgcagtca gacacggtgc ccctcctgca gaatggccag
      361 catgcccgca gccagcctcg ggcttcaggc cctcctcggt ccatccagcc acaggtggcc
      421 ccgaggcaga gggtgcagcg ctcccagcct gtgcacatcc tcgctgtcag gcgccttcag
      481 gaggaagacc agcagtttag aacctcatct ctgccggcca tccccaatcc ttttcctgaa
      541 ctctgtggcc ctggggcccc ccctgtgctc gcgccgggtg ctttacctcc gagccaggcc
      601 gccgcaaagc aggatgttaa agtctttagt gaagatggga caagcaaagt ggtggagatt
      661 ctagcagaca tgacagccag agacctgtgc caattgctgg tttacaaaag tcactgtgtg
      721 gatgacaaca gctggacact agtggagcac cacccgcacc taggattaga gaggtgcttg
      781 gaagaccatg agctggtggt ccaggtggag agtaccatgg ccagtgagag taaatttcta
      841 ttcaggaaga attacgcaaa atacgagttc tttaaaaatc ccatgaattt cttcccagaa
      901 cagatggtta cttggtgcca gcagtcaaat gnatcaccac ttgaaatttg at
//
