LOCUS       sequence-49884-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pCA-T-intron-G
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(178..199)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     527..546
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    698..731
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(809..828)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      121 gaattcaggg atatccttgt aaagctcgtc catgccgaga gtgatcccgg cggcggtcac
      181 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      241 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      301 ctggtagtgg tcggcgagct gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt
      361 cgccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      421 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      481 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      541 gtcgtccttg aagaagatgg tgcgctcctg gacgtaccct aagggtggga aaatagacca
      601 ataggcagag agagtcagtg cctatcagaa acccaagagt cttctctgtc tccacatgcc
      661 cagtttctat tggtctcctt aaacctgtct tgtaaccata acttcgtata atgtatgcta
      721 tacgaagtta tagatctttg ataccaacca tggtggcgaa cccgggcccg cggtatcaaa
      781 tcaattcttt gccaaaatga tgagacagca caataaccag cacgttgc
//
