LOCUS       sequence-49887-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pExG
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..162)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(169..198)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(205..234)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(327..350)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(525..544)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     794..812
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagaa
      121 ttctcaggat ctcaggtcct cctcgctgat cagcttctgc tcggagttga gatcctcttc
      181 ggaaatgagt ttctgttcgc tgttcaggtc ctcctcgcta atgagtttct gctctaagga
      241 tctcttgtac agctcgtcca tgccgtacag gaacaggtgg tggcggccct cggagcgctc
      301 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggtgtccac
      361 gtagtagtag ccgggcagtt gcacgggctt cttggccatg tagatggtct tgaactccac
      421 caggtagcgg ccgccgtcct tcagcttcag ggcctggtgg atctcgccct tcagcacgcc
      481 gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcattac
      541 ggggccgtcg ggggggaagt tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc
      601 ctgcagggag gagtcctggg tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg
      661 ctcccacttg aagccctcgg ggaaggacag cttcttgtaa tcggggatgt cggcggggtg
      721 cttcacgtac gccttggagc cgtacatgaa ctggggggac aggatgtccc aggcgaaggg
      781 cagggggccg cccttggtca ccttcagctt ggcggtctgg gtgccctcgt a
//
