LOCUS       sequence-49892-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N BKV ER
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactccttc tctggcgccg gaattagatc tgccaccatg gactacaagg atgacgatga
       61 caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag gttatcaaac
      121 aagtttgtac aaaaaagcag gcatggataa agttcttaac agggaagaat ccatggagct
      181 catggacctt ttaggccttg aaagagctgc ctggggaaat cttcccttaa tgagaaaagc
      241 ttatttaagg aagtgtaagg aatttcaccc tgacaaaggg ggcgacgagg ataaaatgaa
      301 gagaatgaat actttgtata aaaaaatgga gcaggatgta aaggtagctc atcagcctga
      361 ttttggaacc tggagtagct cagaggtttg tgctgatttt cctctttgcc cagataccct
      421 gtactgcaag gaatggccta tttgttccaa aaagccttct gtgcactgcc cttgcatgct
      481 atgtcagctt agattaaggc atttaaatag aaaattttta agaaaagagc ccttggtttg
      541 gatagattgc tactgcattg actgcttcac acagtggttt ggcttagacc taactgaaga
      601 aactctgcaa tggtgggtcc aaataattgg agaaactccc ttcagagatc taaagcttta
      661 aggtaactaa cttatattta gataaataat aaaatattaa aaggccctaa gtaattattt
      721 tttttatagg tgccaaccta tggaacagaa gagtgggagt cctggtggag ttcctttaat
      781 gaaaaatggg atgaagattt atttt
//
