LOCUS       sequence-49893-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N MCV ER
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcctccttct ctaggcgccg gaattagatc tgccaccatg gactacaagg atgacgatga
       61 caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag gttatcaaac
      121 aagtttgtac aaaaaagcag gcatggattt agtcctaaat aggaaagaaa gagaggctct
      181 ctgcaagctt ttagagattg ctcctaattg ttatggcaac atccctctga tgaaagctgc
      241 tttcaaaaga agctgcttaa agcatcaccc tgataaaggg ggaaatcctg ttataatgat
      301 ggaattgaat accctttgga gcaaattcca gcaaaatatc cacaagctca gaagtgactt
      361 ctctatgttt gatgaggtca gtacaaaatt tccttgggaa gaatatggaa ctttaaagga
      421 ttatatgcaa agtggatata atgctagatt ttgcagaggt cctgggtgca tgcttaagca
      481 acttagagat tctaagtgcg cttgtattag ctgtaagttg tctcgccagc attgtagtct
      541 aaaaacttta aagcaaaaaa actgtctgac gtggggagag tgtttttgct atcagtgctt
      601 tattctttgg tttggatttc ctcctacttg ggaaagtttt gactggtggc aaaaaacttt
      661 agaagaaact gactactgct tactgcatct gcaccttttc tagactccta cttccttcct
      721 ctgtaagtat tagatatgga aaagtctata aggcaaaana tcaaagaaag gttatttatg
      781 acagattttc tgtactttcc catctaggtt gacgaggccc ctatatatgg ga
//
