LOCUS       sequence-49912-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Tooley AJ, Gilden J, Jacobelli J, Beemiller P, Trimble WS, Kinoshita
            M, Krummel MF
  TITLE     Amoeboid T lymphocytes require the septin cytoskeleton for cortical 
            integrity and persistent motility.
  JOURNAL   Nat Cell Biol. 2009 Jan;11(1):17-26. Epub 2008 Nov 30.
  PUBMED    19043408
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2009-01"; volume: "11(1)"; pages: "17-26. Epub 2008 
            Nov 30"
COMMENT     Sequence Label: pAT206/Sept7shRNA
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(270..287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(270..286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(495..514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     614..636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(674..692)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg gtttatatat
      181 cttgtggaaa ggacgcggga tccggatccg tttgaccaat ttttcaagag aaaattggtc
      241 aaacggatcc ttttttggaa aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
      301 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      361 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      421 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      481 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      541 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      601 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      661 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      721 g
//
