LOCUS       sequence-49919-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Krummel MF, Sjaastad MD, Wulfing C, Davis MM
  TITLE     Differential clustering of CD4 and CD3zeta during T cell 
            recognition.
  JOURNAL   Science. 2000 Aug 25;289(5483):1349-52.
  PUBMED    10958781
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-08-25"; volume: "289(5483)"; pages: "1349-52"
COMMENT     Sequence Label: CD3Zeta-GFP
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(582..681)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(611..631)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctacca tggtggcgac cggtgggcga ggggccaggg tctgcatatg
       61 cagggcatca taggtgtcct tggtggcagt gctgagaccc tggtaaaggc catcgtgccc
      121 cttgcctctc cgcctctcgc cttttgtgcc gatctcactg taggcttctg ccatcttgtc
      181 tttctgcagt gcattgtata cgccttcctg ggggttcctc ctcctctgct gtttgcctcc
      241 catctctgga tcccgagccc gcttcttctc caagacgtca tattcctctc ttcgccctag
      301 attgagctca ttgtagagct ggttggggtc ctgcaggttg gcagcagtct ctgcactcct
      361 gctgaatttt gctctcaggt acagggctgt gatgatgact ccgtagatga agaggattcc
      421 atctagcaag tagcagagtt tgggatccag cagaccaaag ctctgtgcct ctgctcctgg
      481 gaaccgcacg tggaggatgc aggcgagaac agacactttc cacttcatct tctcgagatc
      541 tgagtccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc
      601 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      661 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      721 cttggaaatc cccgtgagtc aaaccgctat ccacgccc
//
