LOCUS       sequence-49926-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Kim KH, An DR, Song J, Yoon JY, Kim HS, Yoon HJ, Im HN, Kim J, Kim 
            do J, Lee SJ, Kim KH, Lee HM, Kim HJ, Jo EK, Lee JY, Suh SW
  TITLE     Mycobacterium tuberculosis Eis protein initiates suppression of host
            immune responses by acetylation of DUSP16/MKP-7.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr
            30.
  PUBMED    22547814
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-05-15"; volume: "109(20)"; pages: 
            "7729-34. Epub 2012 Apr 30"
COMMENT     Sequence Label: pET28b-Rv2416c_wild
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..203
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             207..239
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctggt gccgcgcggc agccatatgg ctagcatgac tggtggacag caaatgggtc
      241 gcggatccat gccacagtcg gattctgtga ctgtgaccct gtgtagcccg accgaggacg
      301 actggccggg gatgttccta ctggccgcgg ccagtttcac cgatttcatc ggccctgaat
      361 cagcgaccgc ctggcggacc ctggtgccca ccgacggagc ggtggtggtc cgcgatggtg
      421 ccggcccggg ttctgaggtg gtcgggatgg cgctgtacat ggatctgcgg ttgacggtgc
      481 ctggtgaagt ggtgctcccg accgccggtc tcagtttcgt cgcggtggcg ccgacgcatc
      541 gccggcgcgg cttgctgcgc gcgatgtgcg ccgaactgca ccgccgcata gccgattccg
      601 gctatccggt cgcggcactg catgctagcg agggcggcat ctacggccgg ttcggctacg
      661 ggcccgctac caccttgcat gagctgacgg tcgaccgacg cttcgcgcgc
//
