LOCUS       sequence-49928-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pCA-T-intron(Neo)-G(LNL)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    116..149
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    335..382
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     407..426
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(725..744)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agggtgggaa atagaccaat aggcagagag agtcagtgcc tatcagaaac ccaagagtct
       61 tctctgtctc cacatgccca gtttctattg gtctccttaa acctgtcttg taaccataac
      121 ttcgtataat gtatgctata cgaagttata gatcctgagt aacctgaggc tatggcaggg
      181 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
      241 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
      301 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt
      361 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
      421 gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg
      481 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
      541 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
      601 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
      661 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
      721 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
      781 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
      841 cgccatgggt cacg
//
