LOCUS       sequence-49931-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pBT259_(pRosa26-G-tTA2)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..622
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          699..764
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cttctcatct ccgggccttt cgacctgcag ggtcctcgcc atggatcctg atgatgttgt
       61 tgattcttct aaatcttttg tgatggaaaa cttttcttcg taccacggga ctaaacctgg
      121 ttatgtagat tccattcaaa aaggtataca aaagccaaaa tctggtacac aaggaaatta
      181 tgacgatgat tggaaagggt tttatagtac cgacaataaa tacgacgctg cgggatactc
      241 tgtagataat gaaaacccgc tctctggaaa agctggaggc gtggtcaaag tgacgtatcc
      301 aggactgacg aaggttctcg cactaaaagt ggataatgcc gaaactatta agaaagagtt
      361 aggtttaagt ctcactgaac cgttgatgga gcaagtcgga acggaagagt ttatcaaaag
      421 gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc ttcgctgagg ggagttctag
      481 cgttgaatat attaataact gggaacaggc gaaagcgtta agcgtagaac ttgagattaa
      541 ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat gagtatatgg ctcaagcctg
      601 tgcaggaaat cgtgtcaggc gatctctttg tgaaggaacc ttacttctgt ggtgtgacat
      661 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
      721 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
      781 ctgatgaatg ggagcagtgg tggaatgcag atcctagagc tcgc
//
