LOCUS       sequence-49933-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: RAD Module G8-C1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..140)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(317..360)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    363..384
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    385..403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
      121 aacctcttac gtgccgatca acgtctcatt ttcgccagat atcgacgtct aagaaaccat
      181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct
      241 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      301 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      361 tggaattcgc ggccgcttct agagtcccta tcagtgatag agattgacat ccctatcagt
      421 gatagagata ctgagcacaa gcttgcggct ttcacacttg ggggctagcg tgagagccct
      481 ggtagtcatc cgcctgtccc gcgtcaccga tgctacgact tcaccggagc gtcagctgga
      541 gtcttgccag cagctctgcg cccagcgcgg ctgggacgtc gtcggggtag cggaggatct
      601 ggacgtctcc ggggcggtcg atccgttcga ccggaagcgc agaccgaacc tggcccggtg
      661 gctagcgttc gaggagcaac cgtttgacgt gatcgtggcg taccgggtag atcggtt
//
