LOCUS       sequence-49935-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N JCV mad1 ER
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactccttc tctggcgcca ggaattagat ctgccaccat ggactacaag gatgacgatg
       61 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      121 caagtttgta caaaaaagca ggcatggaca aagtgctgaa tagggaggaa tccatggagc
      181 ttatggattt attaggcctt gataggtctg catgggggaa cattcctgtc atgagaaaag
      241 cttatctgaa aaaatgcaaa gaactccacc ctgataaagg tggggacgaa gacaagatga
      301 agagaatgaa ttttttatat aaaaaaatgg aacaaggtgt aaaagttgct catcagcctg
      361 attttggtac atggaatagt tcagaggttg gttgtgattt tcctcctaat tctgataccc
      421 tttattgcaa ggaatggcct aactgtgcca ctaatccttc agtgcattgc ccctgtttaa
      481 tgtgcatgct aaaattaagg catagaaaca gaaaattttt aagaagcagc ccacttgtgt
      541 ggatagattg ctattgcttt gattgcttca gacaatggtt tgggtgtgac ttaacccaag
      601 aagctcttca ttgctgggag aaagttcttg gagacacccc ctacagggat ctaaagcttt
      661 aaggtaaacc actatttttc ttttgcaggg cattttgttt tttacaataa acttaattgt
      721 ttttttttag gtgccaacct atggaacaga tgaatgggaa tcctggtgga atacatttaa
      781 tgagaa
//
