LOCUS       sequence-49966-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Voronina E, Seydoux G
  TITLE     The C. elegans homolog of nucleoporin Nup98 is required for the 
            integrity and function of germline P granules.
  JOURNAL   Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24.
  PUBMED    20335358
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2010-05"; volume: "137(9)"; pages: "1441-50. 
            Epub 2010 Mar 24"
COMMENT     Sequence Label: pEV9.03
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..82
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     66..82
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(663..687)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(722..738)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(723..739)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(763..782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(764..782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(792..809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(792..808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(801..823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 actataggga gaccggcaga tctgatatca tcgatgaatt cgagctccac cgcggtggcg
       61 gccgctctag aactagtgga tccaccggtt ccatggctag ccacgtgacg cgtggatccc
      121 catcgaacca ctttgtacaa gaaagctggg tgtgagcctg aattactcgg agccgccggc
      181 gcatcagact ttttcgtcca gaaatccttg gtgggctccg gaaatttgat cgtcgtagcc
      241 ggaatcttca cactcgacgt ggcggcttgt ccgtcttcgc ccgatttgaa aaccactgat
      301 tcagctccac cgagaggtcc tccgccacga atcgcgcgtt ttcgggcggt cgctggagct
      361 gctggagcaa cgagtggctc tgcggttttt gcgagtggag ctggcgtttc cttggttatc
      421 actgaaatgt cgccaaactt gggcacagac gcgactgtcg ccgccgggct cgaggcagcg
      481 aaaactgtag aattcagcgg attttttggc gttccattct tccgcacagc tttggctatt
      541 ttccgcggag caactgtttc tccagaactt tcagacgagt tttcggttcg tctgggcact
      601 gtcatatcgg ctgcaacgtc atcatcgacc ttataaatct gcttgagctg tgccacatga
      661 tcagcctgct tttttgtaca aacttgtttg atgggctgca ggaattcgat atcaagctta
      721 tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat
      781 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      841 caaacttaat cgccttgcag cacatccccc ttt
//
