LOCUS       sequence-49968-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N JCV-cy ER
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattagat ctgccaccat ggactacaag gatgacgatg
       61 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      121 caagtttgta caaaaaagca ggcatggaca aagtgctgaa tagggaggaa tccatggagc
      181 tcatggattt attaggcctt gatagatctg catggggaaa cattcctgtc atgagaaaag
      241 cttatctgaa aaaatgcaaa gaacttcacc ctgataaagg tggggacgaa gacaaaatga
      301 agagaatgaa ctttttatat aaaaaaatgg aacaaggtgt aaaagttgct catcagcctg
      361 attttggtac atggaatagt tcagaggttg gttgtgattt tcctcctaat tctgatactc
      421 tttattgcaa ggaatggcct aattgtgcca ctaatccttc agtgcattgc ccctgtttaa
      481 tgtgcatgct aaaattaagg catagaaaca gaaaattttt aagaagcagc ccacttgtgt
      541 ggatagattg ctattgcttt gattgcttca gacaatggtt tgggtgtgac ttaacccaag
      601 aagctcttca ttgctgggag aaggttcttg gagacacccc ctacagggat ctaaagcttt
      661 aaggtaaacc acaatttttc ttttgcaggg cattttgttt tttacataaa ctgaattttt
      721 tgtttttagg tgccaaccta tggaacagat gagtgggaat cctggtggaa tacatttaat
      781 gagaagtggg atgaaa
//
