LOCUS       sequence-49971-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N WU ER
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattagat ctgccaccat ggactacaag gatgacgatg
       61 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      121 caagtttgta caaaaaagca ggcttcatgg ataaaacttt gtccagaaat gaagcaaaag
      181 aacttatgca gctgctgggt cttgatatga cctgctgggg aaatttacca ctaatgagaa
      241 caaaatacct tagcaaatgc aaagaatttc atcctgacaa agggggaaat gaggaaaaaa
      301 tgaaaaagct taattcttta tatttaaaac tgcaagagtg tgttagtaca gtgcaccaac
      361 taaatgaaga agaagatgaa gtgtggagct cttcacaggt agaatgcaca gaattgtgct
      421 gtaactttcc ccctagaaag tacaggcttg ttggagaagt ttatggtgat gtttttgaag
      481 agtatatttt aaaagactgg gacatatgct taaaggggtt ttattatttg tgtaattgtt
      541 tttactgctt tttagacaag cgccacaagc aaaaatataa aatatttaga aaacctccaa
      601 tgtggataga gtgttactgc tacaggtgct atagagagtg gnttggcttt gaaattagtg
      661 cagaaacatt tttttactgg aanaagatta tatttcttac aaccatgcaa ggggttggct
      721 taaccaggta agcatgtttt tattataggg gtacttttat gttttataag taaattaata
      781 actttttttt
//
