LOCUS       sequence-49973-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N HPyV6
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             685..696
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattagat ctgccaccat ggactacaag gatgacgatg
       61 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      121 caagtttgta caaaaaagca ggcatggatc gccttttagc cagagaagaa gttagggagc
      181 taatggatct tataggcctc agcatggcct gttggggtaa tttacccctt atgcaacaga
      241 agataagact agcttgtaaa aaatatcatc ctgataaagg aggggatcct gaaaaaatgc
      301 aacgcctgaa tgttttaaag gagaagttga atgccaccct gagggaccaa atgtcttcat
      361 cacccacctg gtgcttttcc tctgaggtca gtgatgattg gggtattccc ttaactgttg
      421 gggagttctt aggccctgaa tttcataaaa agaaagtttg ggatttccgt ttgtgtgtac
      481 aacaaggaat cagttcctgc aaatgccttc attgccttct caaaaaggag cataaaaagc
      541 aagtggagat taatttagga aagccaacta tttggggaaa gtgctggtgc tacaagtgct
      601 actgtctctg gtttggccta cctgtggaag ctgantcttt tatgtggtgg acccacatta
      661 tctaccaaag ccccctggat tggttgggaa ttacagagaa gctcatctgg tggtaagtgc
      721 aatttttata gatagtccag gtagtgatgc attgaaact
//
