LOCUS       sequence-50087-e        1572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00552
FEATURES             Location/Qualifiers
     source          1..1572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgatgcaaa ccctgaaacc accgccaccg ctacatcatc accaccagca tcagcaccag
       61 ctccagcatc cgcctcagca gcagccacat ccgcatcagc agctgcaaca tccgttgcag
      121 cagcaacagc acctgtcagc catggataat caccagcaac atcagccgca tatccatcag
      181 cagcagcagc agcagcagca acaattgcca cccacgccgc aggcatctca cctggtcggt
      241 gggcagcaac agcagcagca acatccgcac cacaatcacc tggctgtcga ccaggatgac
      301 ccaaatcccg aactggtgct cgccttaatt tccaggaacc gggcgctcga ggccacaatc
      361 ccgaaatgcg gcggctgcca cgagctgatc cttgaccgct tcatcctcaa agtgctggag
      421 aggacgtggc acgccaagtg cctgcagtgc agcgagtgcc atggccagct gaacgacaag
      481 tgcttcgcgc ggaatggcca gctcttctgc aaggaggact tcttcaagtc gaacagacgt
      541 tacggtacaa agtgttccgc ttgtgatatg ggcattccgc ccacgcaggt ggtgcgtcgg
      601 gcgcaggaca acgtctacca tctgcagtgc ttcctgtgcg ccatgtgctc ccgcaccctg
      661 aacaccggcg acgagttcta cctgatggag gatcgtaagc tgatctgcaa gcgggactac
      721 gaggaggcca aggccaaggg cctctatctg gatggctcgt tggacggcga tcagccgaat
      781 aagcgtccgc gcaccacgat caccgccaag cagctggaga ccctgaaaac ggcctacaat
      841 aacagcccca aacccgcccg ccatgtacgt gagcagcttt cgcaggacac gggcctggac
      901 atgcgggtgg tgcaggtctg gttccagaac aggcgggcca aggagaagcg tctgaaaaag
      961 gatgcgggcc gcacgcgctg gagccagtac ttccgctcga tgaagggcaa ctgctcgccg
     1021 cgcaccgaca aattcctcga caaggatgag ctgaaggttg actacgacag cttcagtcac
     1081 cacgatctga gcaacgacag ctacagcacc gtcaatttgg gcctggatga gggcgcctcg
     1141 ccgcacagca tccgcggatc ctacatgcac ggcagttcga gtccgtcaca gtatccgccg
     1201 agcagtcgct cgccgccgcc agttggccag ggccacacgt tcggctccta tccggacaac
     1261 atagtgtaca ccaacataga tcaagcggtc gggtctagtt tgcatgccag taaggcgcat
     1321 caccgcctcc acagtagcaa caacgtctcc gatctgagca acgattccag tcccgatcag
     1381 ggataccccg actttccgcc cagccccgac tcctggctgg gcgattcggg cagcaccaac
     1441 acgaccagcg ccaacaacaa tgcgaacaac aacagcagca gcagccacaa caacaacaac
     1501 agtagtggcg ggggaagcgg cggggtgagc gtcagtaccg cccccaatcc gtcggcgccc
     1561 ggcgtacatt ac
//
