LOCUS       sequence-50100-e        1983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00574
FEATURES             Location/Qualifiers
     source          1..1983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             613..630
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atggtcatgt tgcaatcgcc ggcgcaaaag gccagcgata gtgcgtcagc ccaaaacaca
       61 gccgtcggtg gcctgatgag tcccaactcg aacccggact cgcccaagtc caacacctcg
      121 ccggatgtgg cgagtgcgga cagtgtggtg tccggcacag gcggtggcag cactccgcca
      181 gcggccaaga tacccaagtt catcatcagc gccaatggcg cagcggtggc gggaaaacag
      241 gagcaggagc tgaggtactc cctcgaacgg ctcaagcaga tgagcagtga atcgggcagc
      301 ttgctcagcc gcttgagccc cttgcaggag gactcccagg ataaggagaa acccaatcac
      361 aacaacaaca gcagtctaac taatcataat gcaaacagca atacacgtcg ctcgcaatcc
      421 cctccagctt cgatgggatc tgtaagcttc tcctcgccag cccagcagcg aaagctcctg
      481 gaactgaatg ctgtgcgcca cttggcaagg ccggagccac tgcagcatcc tcatgcggcc
      541 ctgctgcagc agcatcccca cttgctgcag aatccgcagt tcctggccgc cgcccagcag
      601 cacatgcacc atcatcagca tcagcatcag catcaccagc atcccgcaca tccgcattcg
      661 catcagcatc cgcatccaca ccctcatccg catccccatc cgcatccgtc ggcggtcttt
      721 catttgaggg ctcccagcag cagcagcact gccccaccct cgccggccac ctcgcccctg
      781 tcgccgccca catccccggc catgcattcc gaccagcaga tgagtccacc catcgctccc
      841 ccccaaaatc cgccacattc ctcgcagcca ccacaacagc agcaggttgc cgcaccatcg
      901 gacatggatc tcgagcggat taagctggtg gctgcggtgg cagcccgcac cactcaagct
      961 tccagcacct ccgccttggc gtccgcctcg aactccgtgt ccaatgccag cacctccatc
     1021 tccaactcca gctccggatc gcccagtgga cgcgacttga gcgactacgg attccgaata
     1081 cagctgggtg gcctggcggc ggcggcagct gctgcggcgg ccacctcgcg tcaaattgca
     1141 gcagccacat acgcgaggag cgacaccagc gaggagctca acgtcgatgg caacgacgag
     1201 gacagcaacg atggatcgca cagcacgccg tcggtctgcc cagtggatct gacgcgatcg
     1261 gtgaacagca gtgctgcggc gaatccgagt tcggcctcca cgagtgcctc cagtgatcga
     1321 gatgcggcca caaagcggct ggccttctcc gtagagaaca tactggatcc gaacaagttc
     1381 acgggcaaca agctgccaag tggtcctttc ggccatccca ggcagtggag ctacgagagg
     1441 gacgaggaaa tgcaggagcg attggatgac gatcagagcg aggatatgtc tgcccaggat
     1501 ttgaatgaca tggatcagga cgatatgtgt gacgatggca gcgatatcga cgatcccagc
     1561 agcgagacgg actccaaaaa gggaggcagt cgtaatgggg atggaaagtc cggaggtggc
     1621 ggcggaggtg gttcaaagcc tcgacgagcc cgcaccgcct tcacgtacga acaactagtt
     1681 tccctggaga acaagttcaa gaccaccaga tatctcagcg tctgcgagcg actgaacttg
     1741 gccctcagct tgagcctgac agagacgcag gttaaaattt ggttccagaa ccgccgcacc
     1801 aagtggaaga agcagaaccc cggcatggat gtcaactccc ccaccatccc cccgcccggc
     1861 ggcggctcct tcggaccggg ggcatatgcc agcggactcc tgtactcgca cgcggtgccc
     1921 tatccgccat acggccccta cttccacccc ctgggcgcac atcacctcag ccactcgcac
     1981 tca
//
