LOCUS       sequence-50176-e        3798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00699
FEATURES             Location/Qualifiers
     source          1..3798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             215..232
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgtccacag ataatcccac acagttgctg accacccgtg atttgagcca actgcgtgga
       61 catctgcaat ccggaacagt agcggtgccc tccacgacca cttctgtggt ggcagcagca
      121 gcagcaacag tggctgccgc ccatggatac cgcaccattg caccacccca acaacccgcc
      181 aacaacagca gcacaacaac atctgcaaca gcagcatcag catcagcatc agctcaacca
      241 cctgtaatac caccagttat atccacaagc aatagcaaca tgccggcaag tgcctcaaaa
      301 tatgtatttc tgcaacacaa tcacaatggt ggttttacgg catacgatgg ctctgcagcc
      361 acaacagcgg cgggcagcac cggaactgta ggtgcaacca gtggcaatat agtactactg
      421 gcagccgcac cgctggaagc cagttccggc gaggctttgg tcaatgtgcg aacaactgaa
      481 gatgatggcg agttgcgttc actgtcctgg ctcagcgata ccaatttaat taagggcatt
      541 gtgaccacaa cggccggaca ggcaggaaaa cgtgctgcaa cggtggccat caagtcggcg
      601 actggggcag caggcacagt aggatccggt gctcccaatg gcaatctgtg tatagtcagc
      661 gatttaatag aagagctacc cactaatctg agtgaaacca gtgaacagga gtcgaccgga
      721 ggagcagcaa gtggatcggc accagcagca tcaactgtgt acagcaccac aacggtgcac
      781 aaaggagcca gtggcaccac caccgtggtg gagtacaagg ccagcaaagc caatcagcag
      841 caacaaaatt cgccgcaaca gcagcagcag cagcagcagc agcaacaaca acaacaacaa
      901 caacaacaac aacaacaaca acaacaacaa caacaacaac aacagacgac ctatttgcca
      961 acgccgacaa gaaccaccat ggtgggtcac ggctatatgc cggtagtggt gagcagtgcc
     1021 agccaaccca tattatcgcc cgccaaacag cagctaccgc aacagattta cgttaccagc
     1081 aatggcagtt cgccagccgg cggaggaacc atctacaagg cagccagcca ggcacttagc
     1141 tcgccaacca ccagcagcag cagcagtagc aacaccacca cccaccatcc gcacaagaaa
     1201 tatctgcgcg aaaagatgca cgtccagatg gatcacagca gtcatgtggc ctcaaccgta
     1261 acggtggtca gttcgccagc atcctcgaac aattcctcga ttagcagctc caacacaggc
     1321 tcggtatccg gttcgggttc ctcgacagcg gctgtgaatg gtggcaatag cccagtgccc
     1381 aagaccagta gctttagcac cgtattcgag gcggtcaact atgccacatc ggcggccagc
     1441 aatagcagca gcagcagcaa cagtacaccc aacagtcaag ccatactcca tggccaagag
     1501 tcgccggctg tggtgaccac aacgacattg atcagtgccg gtaccctgcc gccgggctat
     1561 agcttcgtca atcaagtggc cagcacgccg catgtgcttt ctacaccagc gcatgttgca
     1621 tctccggaga cgccacccgg agcggctttg ttgccgggaa cttattatgc cacaagcacc
     1681 acatcagcgg gcacaaccac aaccactttg caggcgcgta gtctgcagtt atccgtctcg
     1741 ccaccgccgc caaatatgac gcccacgagt caagtgcaca atggatcact aggaggagga
     1801 ggaggagggg gaggaggatc tggaggagga ggaggaggag gaggagccaa cctgcggagt
     1861 cctaatagct atgccagcta cgataacgag gattccctca aggaattcga tctggtggtc
     1921 agctcacgct tgcacacaag cacgccgcaa tattcagtgt ccaaaaatgg agccaacggc
     1981 tatggtaacg gcaacagcaa tgtgaatggc agcggagctg gttccaatac gccgcaaaag
     2041 caaaagcatc ccaataacgt gccatacgat ccgttagtgc ataccaataa caagccgcca
     2101 tatagcttta gttccttgat cttcatggcc attgagggtt cgaatgagaa ggcactgccc
     2161 gttaaagaga tctatgcgtg gattgtccag cactttccgt acttcaagac ggctcccaat
     2221 ggctggaaaa atagcgtgcg ccacaatctc tcgctgaaca agagctttgt caaggtggag
     2281 aaggctccaa acatgggcaa gggatcgttg tggcgtgtgg agccacagca gcgccagaat
     2341 ctcatccagg cgctgaaccg ttcgccattc tttcccaact cggcggtgga taaaattagt
     2401 ccatcgctta agagtcccag tggtggatcc gcatacgata gcctcgatgg cggtggcagt
     2461 ggttccgttt cttcggctca gccagtggct ggtggtgctg gagttccggc tgccgcagcc
     2521 gtggcactgt ccacgcccac caaaagcaat ggtctggcgc tggccaatgg tgctagtcag
     2581 gccacaaacg cggccaggcc gcatagtccc aatggtggtg gcagtggcag tcatgcccgt
     2641 ttcgatccct acctattccc caatctgtcc aaagcttttc gcaatatacg cgaagatacg
     2701 gtcgggcagc caatgctgca agatgccctc gatgacgagc tgtccggcga ttatcacaat
     2761 aataataaca ataatagtgg cagcaagtac aattacatgg gtgcgaatgg gtcaggtgga
     2821 gctggcagcg gtggtgttgg gtccaacgca aatggagctt ccgacggcat caatttcgcg
     2881 cgactggccc gcgattgcgg agcggacagc attgatgatg tgcatgcggc ggcggcgatg
     2941 ctctacctca aacacggacc aaagatctat agtgagccct tccagaatgg tagtggcccg
     3001 gtgataacga gctcgccaag cgaggatcac acctactcgg cgggtggcaa cagcaatgcg
     3061 gacagcggct cctccacgcc actgaccaat ggcaatgctc tggccagcgt ggcgcaagca
     3121 gtggcccagg gtcagaataa ccagggagga gctggatccg atagcaattg cgccagctcg
     3181 gatgctgcct acgatagcag tgaggagaat cacaacatca cgcccgagga gatggccgat
     3241 cggcagcgac atcgcgacgg cgtggacgcc ctgctctcgc tatccggctc ctcgattgtg
     3301 gagtgcggct cagtggccac cacacattat cacagcaacg gcagcagcgg cagcagccat
     3361 ggatccccgc acaagcgagc ctcgagtcac agtttggagg aggagcacct gcagcagcac
     3421 agggaacagc agttgcagca acagcaacag cagcagcaac atcatcacca gcaatttggc
     3481 agcagccaag cggtctacac caatggcagt ggcagcaaga tggctttatt gagcagtgct
     3541 gccgccaatg tggcactcgc tcaacagcag cagcagcaga ttcaccagga catgtactcc
     3601 tcgacggctg gacacaatgc cagtatgtat ctgggcgggc tgaacagtca ctgcctgtcc
     3661 ggtggcggcg gtgtgggcgt ggcggcgggc atgttgccac agcatttgag tgctgcaacg
     3721 ggggcaagta ataaaaataa ggtgaaacca ttgcgaggat tacgcaccaa gttcaagcga
     3781 aagtccgcct ggatgcgg
//
